DNBSEQ-G50 is a sequencer designed for lower throughput and faster turnaround times. The DNBSEQ-G50 adopts two colour chemistry and DNBseq to support rapid sequencing in various different read modes.
- Zero accumulation of error in amplification, with high-precision base read-through
- 4 types of reads are available. Running the PE100 process at full load takes only 48 hours
- Two flow cell options of 100M reads or 500M reads
- Flexible data yield from 10Gb to 150Gb
- Sequencing time of 10 hours to 64 hours
| Total Reads | FC 300 |
|---|---|
| Read Lengths | SE 50 |
| Read Lengths | SE 100 |
| Read Lengths | PE 50 |
| Read Lengths | PE 100 |
| Turnaround Time | SE 100: 300M 26 HRS |
| Turnaround Time | PE 100: 300M 48 HRS |
| Turnaround Time | SE 100: 100M 10 HRS |
| Turnaround Time | PE 100: 100M 48HRS |
Performance Parameters
| Chip types | FC | Average Effective Signal Point | 300M | Average Output | 15-60Gb/run |
|---|---|---|---|---|---|
| Chip lanes number | 1 lane | Read length * | SE50 SE100 PE50 PE100 | Run time ** | 48 hours or less |
** Running at full load PE150 takes 64 hours per run
Non-accumulative amplification errors
High precision base reading
By employing DNA Nanoballs {DNB) technology and its special linear amplification method, DNBSEQ-G50 effectively carries out high-rate amplification, while ensuring a reduced error rate.
Moreover, its nanochip spot design allows for signal enhancement while ensuring sequencing accuracy without signal interference. MGISEQ-200 uses improved combinatorial Probe-Anchor Synthesis (cPAS) technology to incorporate a fluorescent probe to a DNA anchor on the DNB. A high-resolution imaging system is then used to collect, read and identify optical signals.
This sequencing system not only avoids highly repetitive single-base reading errors caused by common physical signal errors, but it also produces high quality and high accuracy sample sequencing information.
Flexible selection of read lengths
Rapid sequencing
DNBSEQ-G50 has been specifically designed for its temperature regulation system and sequencing enzyme. It has been possible to increase the number of types of supported read lengths without affecting the size of the device.
In addition, in order to meet the requirements for flexibility of throughput and time in different application scenarios, DNBSEQ-G50 has undergone improvements in the time required to carry out sequencing in different rend length modes. For example, a PE100 run can be completed using the FCS 100M read flow cell in 28 hours, and the FCL-500M flow cell will be completed within 45 hours.
The full list of read lengths are SE50, SE100, PE100, and PE150.
| Flow cell | Read length | Data output | Q30% | TAT |
| FCS-100M | SE100 | 10G | >80% | ~10h |
| PE100 | 20G | >80% | ~28h | |
| PE150 | 30G | >75% | ~41h | |
| FCL-500M | SE50 | 25G | >80% | ~11h |
| SE100 | 50G | >80% | ~18h | |
| PE100 | 100G | >80% | ~45h | |
| PE150 | 150G | >75% | ~64h |
FASTQ data output
DNBSEQ-G50 uses the universal FastQ data format, supporting local analysis, cloud analysis, and many other analytical methods. With such breakthroughs to operating conditions. DNBSEQ-G50 is aimed at bringing gene sequencing to more regions and being accessible to more people.