MGISEQ-200

MGISEQ-200 is a sequencer designed for lower throughput and faster turnaround times.  The  MGISEQ-200 adopts two colour chemistry and DNBseq to support rapid sequencing in various different read modes.

  • Zero accumulation of error in amplification, with high-precision base read-through
  • 4 types of reads are available. Running the PE100 process at full load takes only 48 hours
  • Two flow cell options of 100M reads or 500M reads
  • Flexible data yield from 10Gb to 150Gb
  • Sequencing time of 10 hours to 64 hours

Performance Parameters

Chip typesFCAverage Effective Signal Point300MAverage Output15-60Gb/run
Chip lanes number1 laneRead length *SE50 SE100 PE50 PE100Run time **48 hours or less

** Running at full load PE150 takes 64 hours per run

Non-accumulative amplification errors

High precision base reading

By employing DNA Nanoballs {DNB) technology and its special linear amplification method, MGISEQ-200 effectively carries out high-rate amplification, while ensuring a reduced error rate.

Moreover, its nanochip spot design allows for signal enhancement while ensuring sequencing accuracy without signal interference. MGISEQ-200 uses improved combinatorial Probe-Anchor Synthesis (cPAS) technology to incorporate a fluorescent probe to a DNA anchor on the DNB. A high-resolution imaging system is then used to collect, read and identify optical signals.

This sequencing system not only avoids highly repetitive single-base reading errors caused by common physical signal errors, but it also produces high quality and high accuracy sample sequencing information.

Flexible selection of read lengths

Rapid sequencing

MGISEQ-200 has been specifically designed for its temperature regulation system and sequencing enzyme. It has been possible to increase the number of types of supported read lengths without affecting the size of the device.

In addition, in order to meet the requirements for flexibility of throughput and time in different application scenarios, MGISEQ-200 has undergone improvements in the time required to carry out sequencing in different rend length modes. For example, a PE100 run can be completed using the FCS 100M read flow cell in 28 hours, and the FCL-500M flow cell will be completed within 45 hours.

The full list of read lengths are SE50, SE100, PE100, and PE150.

Flow cell Read length Data output Q30% TAT
FCS-100M SE100 10G >80% ~10h
PE100 20G >80% ~28h
PE150 30G >75% ~41h
FCL-500M SE50 25G >80% ~11h
SE100 50G >80% ~18h
PE100 100G >80% ~45h
PE150 150G >75% ~64h

FASTQ data output

MGISEQ-200 uses the universal FastQ data format, supporting local analysis, cloud analysis, and many other analytical methods. With such breakthroughs to operating conditions. MGISEQ-200 is aimed at bringing gene sequencing to more regions and being accessible to more people.