Dovetail™ Hi-C Kit

The Dovetail™ Hi-C Kit uses a single restriction enzyme (DpnII) for chromatin fragmentation prior to proximity ligation. The kit provides all the reagents necessary for performing the proximity ligation steps on animal, plant and prokaryotic samples prior to NGS library generation. The kit is compatible with the Dovetail™ Library Module for Illumina (Cat # 25004) and 3rd party standard paired-end library preparation kits for Illumina sequencing.

Specifications

Reactions8
Validated SamplesMammalian cells, Mammalian tissues, nucleated blood, non-nucleated blood, young plant leaves, insects
TechniqueHi-C
LabellingResearch Use Only
Application(s)Genome Assembly, chromatin conformation analysis, and structural variation detection

Key Benefits

The Dovetail™ Hi-C Kit offers the following key benefits:

  • Proven Hi-C chemistry well accepted in the genome assembly and chromatin conformation research fields
  • 6 hour protocol with less than 3 hours hands-on time easily adopted by molecular biology and genomics labs
  • Single source kit containing specialized reagents necessary to perform the proximity ligation portion of the protocol (customer provides formaldehyde, EtOH, RNase/DNase free water and SPRIselect beads).

Documents

Contents & Storage

Module 1 of 2 (Shipped with cold packs; store 2 to 8 degrees Celsius)

  •  TE Buffer pH 8.0
  •  Wash Buffer
  • TWB Solution
  • 2x NTB Solution
  • LWB Solution
  • NWB Solution
  • Chromatin Capture Beads
  • Crosslink Reversal Buffer
  • Steptavidin Beads
  • 10x RBC Lysis Buffer
  • 20% SDS
  • 40 mM Calcium Chloride

Module 2 of 2 (Shipped with cold packs; store -30 to -10 degrees Celsius)

  •  Restriction Digest Buffer
  • Restriction Digest Enzyme Mix
  • End Fill-in Buffer
  • End Fill-in Mix
  • Intra-Aggregate Ligation Buffer
  • Intra-Aggregate Ligation Enzyme Mix
  • Proteinase K
  • Collagenase
  • HotStart PCR Ready Mix
  • 250 mM DTT