Dovetail™ Omni-C™ Kit

The Dovetail™ Omni-C™ Kit uses a sequence independent endonuclease for chromatin digestion prior to proximity ligation. The Omni-C™ Kit provides all the reagents necessary for performing the proximity ligation steps on plant and animal samples prior to NGS library generation. The kit is compatible with the Dovetail™ Library Module for Illumina (Cat # 25004) and 3rd party standard paired-end library preparation kits for Illumina sequencing.


Validated SamplesPlant and Animal cells and tissues
LabelingResearch Use Only
Application(s)Genome Assembly, Chromatin conformation analysis, SNP and structural variant detection

Key Benefits

The Dovetail™ Omni-C™ Kit offers the following key benefits:

  • Sequence-independent chromatin fragmentation enables genome-wide detection of chromatin contacts (up to 20% of the genome lacks coverage using restriction enzyme based Hi-C approaches)
  • Even genome-wide coverage, similar to shotgun sequencing, enables SNP calling, chromosome phasing, genome assembly, and structural variant detection
  • Lower sequencing burden to reach desired depth of coverage saves time and cost

Chromatin Interactions

Omni-C Libraries Enable Genome Wide Resolution of Chromatin Interactions

Due to the use of a sequence-independent endonuclease for chromatin digestion, the Omni-C Kit offers all the characteristics of a Hi-C approach without the sequence bias inherent to restriction enzyme based Hi-C approaches. Compared to restriction enzyme-based Hi-C approaches, Omni-C provides the following benefits:

  • Significant overlap with data generated using restriction enzyme based approaches but enriched in long-range cis reads
  • Improved resolution when viewing chromatin conformation and looping interactions
  • Most complete view of genome-wide chromatin conformation through dramatically increased resolution of topological interactions that occur in regions with low restriction enzyme density

SNPs & Phasing

SNPs & Chromosome Phasing

The even sequence coverage associated with Omni-C libraries enables genome-wide SNP calling and downstream applications based on SNP information. For example:

  • Improved coverage of Omni-C libraries enables chromosome phasing with low switch error rates
  • Best possible approach for whole-genome physical phasing using Illumina short reads

Large SVs

Large SVs Are Captured In Omni-C Data

The proximity ligation data can be used to detect and confirm chromosomal rearrangements in cancer samples at a high resolution. Using open-source software tools such as HiGlass, contact matrices enable the quick visualization of such large structural variants


Contents & Storage

Module 1 of 2 (Shipped with cold packs; store 2 to 8 degrees Celsius)

  • TE Buffer pH 8.0
  • 10x Wash Buffer
  • TWB Solution
  • 2x NTB Solution
  • LWB Solution
  • NWB Solution
  • Chromatin Capture Beads
  • 10x Crosslink Reversal Buffer
  • Strepavidin Beads
  • 10x RBC Lysis Buffer
  • 20% SDS
  • 10% Triton X-100
  • 100 mM MnCl2
  • 0.5 M EDTA

Module 2 of 2 (Shipped with cold packs; store -30 to -10 degrees Celsius)

  • Nuclease Enzyme Mix
  • 10x Nuclease Digest Buffer
  • End Polishing Enzyme Buffer
  • End Polishing Enzyme Mix
  • Intra-Aggregate Ligation Buffer
  • Intra-Aggregate Ligation Enzyme Mix
  • T4 DNA Ligase
  • Bridge
  • 5x Bridge Ligation Buffer
  • 250 mM DTT
  • HotStart PCR Ready Mix
  • Proteinase K