Parallel isolation and downstream analysis of translated RNAs (by RNA-seq or qPCR) and newly synthesized proteins.
AHARIBO is a unique modular system for fast and reproducible isolation of active ribosomes enabling scientists to study the complexity of the cellular translational machinery at multiple layers providing comprehensive data and insight in its regulation at RNA and protein levels.
Unique workflow based upon AHA (L-azidohomoalanine) incorporation and magnetic beads separation makes the system suitable for HT applications
The three compatible modules include Module 1 for RNA analysis, Module 2 for proteome analysis and Module 3 for western blot preparation.
- Translatome analysis
- De novo proteome analysis
- Ribosome heterogeneity studies
- Characterization of lncRNA