How CRISPRclean Works
CRISPRclean mediated depletion is integrated into a stranded total RNA sequencing library prep protocol. After the adapter ligation step, CRISPR-Cas9 complexes are formed with a pool of designed guide RNAs. After targeted sequences are cut, they cannot be substrates for PCR amplification and subsequent sequencing. The refined libraries (non-targeted sequences) is size-selected using magnetic beads. The output is a refined, sequence-ready NGS library.
Benefits
- Highly programmable
- Achieves efficient depletion
- Increased sensitivity
- Simple automatable workflow
Get started with CRISPRclean
Patent Number
US Patent – US 10,604,802 – Genome Fractioning
EP Patent – EP3102722 – Genome Fractioning
CRISPRclean