Parse Biosciences

Parse Biosciences is a Seattle-based company with the mission of accelerating progress in human health and scientific research.

At the core of their company is their pioneering approach for single cell sequencing. Single-cell sequencing has already enabled groundbreaking discoveries which have led to new understandings of cancer treatment, tissue repair, stem cell therapy, kidney and liver disease, brain development, and the immune system. Parse Biosciences provides researchers with the ability to perform single cell sequencing with unprecedented scale and ease.

Technology

Parse Biosciences’ pioneering technology uses combinatorial cDNA barcoding within cells themselves, and thus does not require complex cell partitioning instruments.

Individual transcriptomes are uniquely labeled by passing fixed cells or nuclei through four rounds of barcoding. In each round, pooled cells are randomly distributed into different wells, and transcripts are labeled with well-specific barcodes. Using next-generation sequencing, each transcriptome is assembled by combining reads containing the same four-barcode combination. Four rounds of barcoding can yield 3,538,944 possible barcode combinations (three rounds of barcoding in 48x96x96 wells followed by a fourth round with 8 PCR reactions), enough to uniquely label up to one hundred thousand cells while avoiding doublets.

Products

Introducing the Parse Biosciences Single Cell Whole Transcriptome Kit

The Parse Single Cell Whole Transcriptome Kit is the most scalable single cell RNA-seq solution on the market, allowing you to profile 100,000 cells and 48 samples together in one run.

A pioneering technology used in 50+ labs.

Parse Single Cell Whole Transcriptome Solution image

SCALABLE

Profile up to 100,000 cells with a single kit, which can accommodate up to 48 different biological samples or experimental conditions.

FLEXIBLE

Fix and store samples on your schedule. Run fixed samples collected on different dates together in a single experiment.

NO CUSTOM INSTRUMENT

No need to pay for an expensive instrument and maintenance fees. Kits use only basic laboratory equipment.

Parse Biosciences Software

The Parse Biosciences computational pipeline is an out-of-the-box software tool that you can run locally to convert fastq files straight to processed data (including gene-cell count matrices). Customers purchasing the Whole Transcriptome Kit will receive access to the Parse computational pipeline. 

Summary statistics provide information necessary to diagnose sequencing runs. Interactive plots include a cell cutoff graph, sequencing saturation curves, and visual summaries of transcript and cell distribution across wells during barcoding.  Statistics for each cluster marker are summarized in the differential expression table along with links to the NCBI database, and the interactive UMAP makes it possible to quickly interrogate genes of interest. 

Parse Technology datasets screenshot