Spatial Omics Analysis Suite Overview: ImageStudio, SAW, and StereoMap

ImageStudio:

1. ImageStudio is a SpatioTemporal omics offline image processing software.
2. It assesses image quality, focusing on sharpness and track lines, determining suitability for downstream data analysis.
3. The manual adjustment module addresses scenarios beyond the software’s capabilities.

SAW (Stereo-seq Analysis Workflow):

1. SAW integrates Stereo-seq spatial group gene expression analysis tools.
2. It reduces and visualizes spatial expression information from sequencing data on microarrays.
3. Spatial expression matrices generated by SAW are suitable for downstream analysis.

StereoMap:

1. StereoMap is a high-definition visual desktop software designed for Stereo-seq data analysis results.
2. It visualizes STOmics’ SpatioTemporal omics technologies, allowing further exploration through various tools.
3. GEF matrix, image RPI and IPR data, and clustering results from SAW can be displayed in StereoMap.

STOmics Cloud is a spatial-temporal data analysis platform for managing and analyzing multi-omics data. The platform is centered around projects, enabling users to seamlessly integrate data and tools into their projects, ensuring traceable analysis processes, reproducible results, knowledge sharing, and project collaboration. Additionally, STOmics Cloud offers a user-friendly portal interface, providing code-free data analysis, high-resolution visualization, and personalized analysis services.

STOmics Stereo-seq Transcriptomics for Large Chip Designs (LCD) pioneers the whole transcriptome study for entire tissue sections. Stereo-seq for Large Chip Designs is firstly offered to the market – 1cm x 2cm, 2cm x 2cm, 2cm x 3cm. Compatible for all species (FF samples), it enables a “tissue-to-data” solution through in situ capture of the whole transcriptome, at nanoscale resolution and centimeter-sized field of view.

Unlock the potential of integrating tissue phenotyping and spatial heterogeneity profiling of molecular information. By incorporating H&E-stained tissue morphological information, Stereo-seq Transcriptomics H&E solution will better assist with tissue type identification, profiling of specific tissue regions of interests (ROIs), and conducting downstream differential analysis between selected ROIs.

STOmics Stereo-seq Transcriptomics Set for Chip-on-a-slide enables a “tissue-to-data” solution through in situ capture of the whole transcriptome, at nanoscale resolution and centimeter-sized field of view. Stereo-seq Transcriptomics Solution utilizes DNB patterned array chips loaded with spatially-barcoded probes that capture and prime poly-adenylated mRNA from tissue sections in situ. Each cDNA synthesized from mRNA captured on a particular spot is linked to its spatially-barcoded probe, allowing subsequent gene expression mapping of a tissue section following sequencing and visualization analysis using the StereoMap visualization platform.

STOmics Stereo-seq Transcriptomics Set for Chip-on-a-slide enables a “tissue-to-data” solution through in situ capture of the whole transcriptome, at nanoscale resolution and centimeter-sized field of view. Stereo-seq Transcriptomics Solution utilizes DNB patterned array chips loaded with spatially-barcoded probes that capture and prime poly-adenylated mRNA from tissue sections in situ. Each cDNA synthesized from mRNA captured on a particular spot is linked to its spatially-barcoded probe, allowing subsequent gene expression mapping of a tissue section following sequencing and visualization analysis using the StereoMap visualization platform.