The Twist Alliance Clinical Research Exome, developed in collaboration with the Broad Institute Genomics Platform, is a versatile tool designed for comprehensive exome sequencing. Utilizing validated data from clinical patient samples, this panel is constructed on Twist’s flexible Next-Generation Sequencing (NGS) platform, allowing easy and rapid customization of content. It provides a thorough examination of the exome, with additional enrichment focusing on clinically relevant areas associated with cancer, rare diseases, and inherited conditions. Leveraging the exceptional uniformity of Twist NGS probes, the assay achieves cost and throughput efficiency. The Broad Institute Genomics Platform has already processed over 250,000 samples, showcasing the panel’s effectiveness and keeping the institute at the forefront of exome sequencing. The panel’s design encompasses the Twist Core exome, the mitochondrial genome, and validated coding and non-exonic regions of interest, including ACMG73 genes, supplemental coverage of OMIM and COSMIC regions, and specific targets defined by Broad. It offers a comprehensive solution for a range of research applications. Important to note, the content of the Twist Alliance Clinical Research Exome – 34.9 MB has not been fully validated through the complete Twist workflow. For guidance on assay set-up, users are advised to contact Twist Customer Support. Additionally, this panel is not ISO-13485 certified.