TrueAmp Polymerase Mix

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Twist TrueAmp Polymerase Mix

The Twist TrueAmp Polymerase Mix is an engineered amplification reagent purpose-built for NGS library workflows — not adapted from qPCR or cloning applications, but designed from the ground up for the demands of complex library amplification. At its core is a proofreading, high-processivity DNA polymerase formulated to deliver uniform coverage across the full GC spectrum, from AT-rich to GC-rich targets, while minimising the amplification-induced errors and homopolymer slippage that compromise variant calling accuracy. Whether you’re amplifying pre-capture or post-capture libraries, TrueAmp Polymerase Mix keeps bias low and your data trustworthy.

Delivered as a single-tube 2× mastermix with aptamer-enabled hot start, TrueAmp Polymerase Mix is stable at room temperature and ready for automated liquid handling — reducing setup complexity, minimising handling errors, and supporting same-day or overnight hybridisation workflows alongside Twist target enrichment kits. It is validated end-to-end with Twist library preparation and enrichment reagents, making it a natural fit for laboratories already running Twist workflows who want consistent amplification performance without introducing an unvalidated variable. As your authorised Australian distributor, Decode Science can advise on integration into your current library prep and enrichment protocols.

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But... Why Choose TrueAmp Polymerase Mix?

Sustaining coverage uniformly across Full GC Spectrum (5 – 95% )

High Sensitivity & Consistent Yields From Low-Input Libraries

Reduced Homopolymer Slippage and Deamination Errors

Automation-Ready Hot Start in a Simplified Single-Tube Format

Product Data: Performance Across the Metrics That Define Library Amplification Quality

Low-Bias Amplification Across GC Extremes

TrueAmp Polymerase Mix sustains normalised coverage across the full GC content range — including AT-rich organisms such as C. difficile and GC-rich organisms such as B. pertussis — where other polymerases show progressive coverage dropout. This performance is maintained as PCR cycle number increases, making it reliable for both low-cycle pre-capture amplification and higher-cycle post-capture workflows.

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Figure 1. GC-normalized coverage; AT-rich C. difficile and GC-rich B. pertussis.

Efficient Yields at Low Input in Fewer Cycles

Improved amplification efficiency means TrueAmp reaches yield targets in fewer PCR cycles than standard NGS polymerases. Fewer cycles directly reduces the accumulation of duplicate reads and cycle-induced artefacts — preserving library complexity and sequencing efficiency, particularly from low-yield post-capture libraries where every cycle counts.

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Figure 2. Serial dilutions from 1 ng to 100 fg, Qubit and TapeStation QC.

Reduced C→T Misincorporation and Homopolymer Slippage

Engineered proofreading activity measurably reduces C→T misincorporations introduced by cytosine deamination — one of the most common sources of false positive variant calls in NGS data, and particularly prevalent in FFPE-derived libraries. Improved homopolymer fidelity further reduces a known source of indel artefacts in repeat-containing regions, supporting cleaner variant calls across the full spectrum of library types.

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Figure 3. Substitution rates after >7M base incorporations; homopolymer readout using Twist clonal plasmids.

Bead-Tolerant PCR for Reliable Post-Purification Amplification

Magnetic beads used for library purification and size selection can carry over into PCR reactions and inhibit amplification, reducing yields and introducing variability. TrueAmp Polymerase Mix is formulated to maintain robust performance in the presence of magnetic bead carryover — removing a common source of batch-to-batch inconsistency in post-purification amplification steps.

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Figure 4. Tolerance to Paramagnetic Beads During PCR. PCR reactions were spiked with 6.25 μl, 12.5 μl, 25 μl, and 50 μl of MyOne T1 Beads, M270 Beads (Invitrogen), and Twist DNA Purification Beads. Reactions were purified post bead-removal and quantified on Qubit dsDNA Broad Range Assay.

Why It Matters to You?

Because Amplification Bias Is One of the Last Variables Most Labs Think to Interrogate

When sequencing results are inconsistent or coverage is uneven, the first instinct is usually to look at sample quality, capture chemistry, or bioinformatics. Amplification polymerase is rarely the first variable examined — but for libraries with challenging GC content or low input, it is often where the problem originates.

TrueAmp Polymerase Mix is most relevant in these contexts:

Target enrichment and exome sequencing —

GC-extreme regions are routinely underrepresented in enriched libraries. TrueAmp’s uniform coverage across 5–95% GC content ensures those targets are captured and sequenced with the same confidence as GC-neutral regions.

Post-capture PCR from low-yield libraries —

After hybridisation capture, the library is often at its most precious and most limited in quantity. An amplification reagent that achieves yield targets in fewer cycles with less bias preserves the complexity and integrity of that material.

Somatic variant detection and low VAF calling —

Reduced C→T misincorporation and homopolymer slippage directly improves the signal-to-noise ratio for low-frequency variant detection — critical for tumour heterogeneity studies, liquid biopsy, and minimal residual disease monitoring.

FFPE-derived libraries —

Deamination damage in FFPE DNA generates artefactual C→T changes that a standard polymerase will faithfully amplify. TrueAmp’s engineered proofreading reduces this signal, preserving the accuracy of variant calls from archival material.

Chris Wicky

Clinical Genomics Manager - ANZ & Country Manager - NZ

Running Twist library prep or enrichment workflows and want to know if TrueAmp Polymerase Mix fits?
 
Our team can assess compatibility with your current protocol and advise on integration.
Get In Touch Today

Related Products

Twist PCR-Free WGS Library Preparation Kit

Bias-free whole genome libraries from high-quality input DNA, no amplification required

View to Know More >

Twist Custom NGS Panels

Design and order target enrichment panels tailored to your gene list or genomic region of interest

View to Know More >

Twist Exome 2.0

Comprehensive exome capture panel with proven uniformity across canonical and difficult targets

View to Know More >

Resources

Twist TrueAmp Polymerase Mix Protocol

Download Twist TrueAmp Polymerase Mix Product Sheet

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    Download Product Sheet

    FAQs

    TrueAmp Polymerase Mix is an engineered proofreading, high-processivity DNA polymerase formulated specifically for NGS library amplification. Unlike standard PCR enzymes optimised for single-amplicon applications, TrueAmp is designed to amplify complex NGS libraries with uniform GC coverage, high fidelity, and consistent yields — minimising the bias and artefacts that standard enzymes introduce when applied to library amplification.

    TrueAmp Polymerase Mix generates consistent, high-yield libraries down to 100 pg input or below. Contact Decode Science for guidance on cycle number optimisation at very low inputs.

    TrueAmp Polymerase Mix is validated end-to-end with Twist library preparation and enrichment reagents. It is compatible with pre-capture and post-capture amplification steps and with same-day and overnight hybridisation workflows alongside Twist target enrichment kits. Contact Decode Science to confirm compatibility with your specific protocol configuration.

    TrueAmp Polymerase Mix has been validated with Twist library prep and enrichment reagents. For use with other library prep systems, contact Decode Science to discuss compatibility and any protocol adjustments that may be required.

    No. The aptamer-enabled hot start provides room temperature stability for setup without compromising PCR performance. It eliminates the need for ice or cold block handling during reaction assembly, simplifying manual workflows and supporting automated liquid handling integration.

    Yes. TrueAmp’s engineered proofreading activity reduces C→T misincorporations caused by cytosine deamination — a common artefact in FFPE-derived DNA — making it particularly well-suited to post-capture amplification from FFPE libraries where deamination damage is elevated.

    TrueAmp Polymerase Mix produces libraries compatible with Illumina sequencing platforms. Contact Decode Science for platform-specific guidance.

    Talk to Us About TrueAmp Polymerase Mix

    We only need these information to serve you better. Decode Science respects your privacy and will never spam you with unrelated content.



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      Twist PCR-Free WGS Library Preparation Kit

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      Twist PCR-Free WGS Library Preparation Kit

      The Twist PCR-Free WGS Library Preparation Kit is engineered for researchers who need their sequencing data to reflect the genome as it actually exists — not as amplification has reshaped it. By eliminating the PCR amplification step entirely, this kit preserves native genome representation from the outset, removing the single greatest source of coverage bias in whole genome sequencing workflows. Built on Twist’s optimised enzymatic fragmentation and high-efficiency ligation chemistry, the workflow delivers consistent insert sizes, minimised ligation bias, and uniform genome-wide coverage that supports confident variant characterisation from the first run.

      Designed to scale from individual research projects to population-level sequencing studies, the PCR-Free WGS kit supports multiplexing of up to 1,536 samples per run via Twist’s full-length unique dual index (UDI) adapters — making it equally suited to high-throughput core laboratories and discovery programmes with large cohort requirements. As your authorised Australian distributor, Decode Science can support you with kit configurations, protocol guidance, and compatibility assessment for your sequencing platform and informatics pipeline.

      Exclusive Offer for now!!

      50% off 16 sample workflow kits & 50% off 96 sample kits. 

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      Product 

      Data

      Why

      It Matters

      Resources

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      FAQs

      Know in Detail

      But... Why Choose PCR-Free WGS Library Prep?

      Amplification-Free Prep for Native Genome

      Minimised Ligation Bias for Maximum Conversion

      Consistent Insert Size Control Across a Wide Input Range

      Scale to 1,536-Plex Without Sacrificing Data Quality

      Product Data: Performance Where It Counts

      Robust Library Yield Maintained at Low Input

      The PCR-Free WGS kit delivers strong library yields and consistent library size distribution even as DNA input is reduced, outperforming competitor PCR-free workflows at equivalent input levels. Efficient ligation chemistry drives conversion across input amounts — producing sequencer-ready libraries that don’t require amplification rescue when input is reduced.

      Download Product Sheet

      Figure 1. PCR-free library yield comparison across inputs. Final library concentration (nM) was measured following PCR-free library preparation using the indicated DNA inputs. Twist PCR-Free WGS Library Preparation generated higher library yields than competitor workflows at equivalent inputs and maintained strong performance at lower inputs.

      Reproducible Insert Size Control From 300 ng to 37.5 ng

      Median insert sizes remain consistent across the full supported input range, from 300 ng down to 37.5 ng. Minimal read overlap across samples in this range confirms that fragment size control is maintained regardless of input quantity — supporting dependable genome-wide coverage without depth fluctuation between samples.

      Download Product Sheet

      Figure 2. The kit produces consistently large inserts across a wide range of DNA samples. (A)Median Insert Size is represented for 300 ng, 75 ng, and 37.5 ng of NA12878 DNA sample input. (B) Percent Overlap (which measures how much paired-end sequencing reads redundantly cover the same DNA bases) is represented for 300 ng, 75 ng, and 37.5 ng of NA12878 sample input.

      Tunable Fragment Lengths for Workflow Flexibility

      Enzymatic fragmentation parameters can be adjusted to produce insert size distributions matched to the requirements of your sequencing platform and read length. This gives laboratories flexibility to optimise the prep for their specific instruments and analysis pipelines without sacrificing inter-batch reproducibility.

      Download Product Sheet

      Figure 3. Tunability of Twist PCR-Free WGS Library Prep Kit.

      A: Five electropherograms of NGS libraries generated using differing fragmentation times. 50 ng of high-quality gDNA was fragmented for various times at 32°C.

      Consistent GC Coverage Across All Input Levels

      One of the most persistent sources of WGS data quality problems is differential coverage across GC content — PCR amplification exacerbates this by further enriching already-accessible fragments. By removing amplification, the PCR-Free WGS kit maintains even coverage across GC content categories regardless of input, yielding cleaner, more interpretable genome-wide data.

      Download Product Sheet

      Figure 4. The Twist PCR-Free WGS Library Preparation Kit provides more consistent coverage distribution across varying GC content. The kit shows minimized GC bias even at low inputs, thereby reducing the need for additional sequencing to achieve uniform coverage and better detection of variants in the high- and low-GC content regions.

      Watch How It Works

      Two steps to sequencer-ready whole genome libraries.

      The PCR-Free WGS workflow is deliberately streamlined — complexity is removed at the chemistry level, not pushed onto the operator.

      Step 1 — Enzymatic Fragmentation: Input DNA is enzymatically fragmented to produce consistent, tunable insert sizes across all samples in a batch. No sonication, no shear-related variability — just controlled, reproducible fragmentation that sets the foundation for uniform downstream coverage.

      Step 2 — Adapter Ligation: Twist’s optimised ligase chemistry maximises adapter conversion efficiency while minimising ligation bias. The result is a sequencer-ready library that accurately represents the molecular diversity of your input, with no amplification step introducing artificial enrichment of any genomic region.

      Why It Matters to You?

      Because Whole Genome Sequencing Is Only as Good as What It Captures

      Whole genome sequencing is increasingly the method of choice for variant discovery, structural analysis, and population-scale genomics — but the value of WGS data depends entirely on whether the library faithfully represents the genome that went into it. Amplification-based workflows introduce systematic biases that are difficult to distinguish from true biological signal, particularly in AT-rich regions, repetitive elements, and low-complexity sequences.

      The PCR-Free WGS kit addresses this directly. Its impact is most relevant for:

      Population and cohort genomics

       Large-scale studies demand per-sample consistency, reproducible coverage distributions, and the ability to scale indexing without index hopping or sample cross-contamination. UDI adapter compatibility and 1,536-plex capacity make this feasible at production scale.

      Structural variant and CNV detection

      Accurate copy number and structural variant calls depend on even baseline coverage across the genome. Amplification-induced regional bias creates false signals that complicate interpretation; PCR-free prep removes this confounder at source.

      Germline variant discovery

      Comprehensive, unbiased genome representation is the baseline requirement for germline variant calling, particularly in regions with extreme GC content or repetitive architecture that amplification-based methods handle poorly.

      High-throughput sequencing cores

      Consistent insert sizes, robust yields across a range of inputs, and multiplexing capacity that scales to 1,536 samples translate directly to higher instrument utilisation and lower per-sample sequencing cost.

      Chris Wicky

      Clinical Genomics Manager - ANZ & Country Manager - NZ

      Planning a whole genome sequencing study or scaling an existing one?
       
      Our team can help you assess input requirements, multiplexing strategy, and expected data output for your specific application. 
      Get In Touch Today

      Related Products

      Twist TrueAmp Library Prep Kit

      High-fidelity amplification-based library prep for target enrichment and challenging low-input or FFPE samples

      View to Know More >

      Twist Custom NGS Panels

      Design and order target enrichment panels tailored to your gene list or genomic region of interest

      View to Know More >

      Twist Exome 2.0

      Comprehensive exome capture panel with proven uniformity across canonical and difficult targets

      View to Know More >

      Resources

      Download PCR-Free WGS Library Prep Product Sheet

      Unlock with quick sign up!

        PCR-Free WGS Library Prep Product Sheet

        Twist PCR-Free WGS Library Preparation Kit Protocol

        FAQs

         Amplification-free workflows are more sensitive to input DNA quality than PCR-based methods, as there is no amplification step to recover yield from degraded material. High molecular weight, intact genomic DNA is recommended. If your samples are degraded or of variable quality, the Twist TrueAmp Library Preparation Kit may be a better fit — contact Decode Science to discuss.

         The PCR-Free WGS kit maintains robust performance from 300 ng down to 37.5 ng DNA input, with consistent insert sizes and sequencing-ready yields across this range.

        The kit is compatible with Twist’s full-length UDI adapter system, supporting multiplexing of up to 1,536 uniquely indexed samples per sequencing run.

        In direct comparisons, the PCR-Free WGS kit delivers higher library yields than competitor PCR-free workflows at equivalent inputs. The absence of amplification removes duplicate reads from the sequencing output, meaning a greater proportion of reads generated are informative — improving effective sequencing depth per run.

         The kit is compatible with Illumina sequencing platforms. Contact Decode Science for guidance on read length optimisation and coverage depth requirements for your specific platform and study design.

         Yes. Amplification-free library prep is particularly well-suited to structural variant and copy number variant analysis, where even baseline coverage is essential. Removing PCR-induced regional enrichment reduces false positive signals in copy number calling.

        The Twist PCR-Free WGS Library Preparation Kit protocol is available from Decode Science on request.

        Talk to Us About PCR-Free WGS

        We only need these information to serve you better. Decode Science respects your privacy and will never spam you with unrelated content.



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          Twist TrueAmp Library Preparation Kit

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          Twist TrueAmp Library Preparation Kit

          The Twist TrueAmp Library Preparation Kit is a precision-engineered solution for next-generation sequencing target enrichment workflows, purpose-built for laboratories that cannot afford to lose data from difficult samples. At its core is the Twist TrueAmp polymerase — a high-fidelity amplification enzyme optimised to suppress amplification-induced errors and GC-related bias, delivering the coverage uniformity your downstream variant analysis demands. Whether you’re working with pristine input or fighting the constraints of degraded FFPE material, TrueAmp is designed to keep your libraries representative and your results reproducible.

          Paired with optimised enzymatic fragmentation and a high-efficiency ligation step, the TrueAmp workflow delivers tunable, consistent insert sizes across a wide range of input quantities — making it well-suited to mixed-input workflows and scaled sequencing operations. From pre-capture yield to on-target coverage, every metric reflects the same design intent: higher library complexity, fewer reruns, and greater confidence in variant calls from the first pass. As your authorised Australian distributor, Decode Science can advise on kit configurations, protocol optimisation, and compatibility with your current target enrichment panels.

          Exclusive Offer for now!!

          50% off 16 sample workflow kits & 50% off 96 sample kits. 

          Submit Your Interest

          Product 

          Data

          Why

          It Matters

          Resources

          Download Instantly

          FAQs

          Know in Detail

          But... Why Choose TrueAmp Library Prep?

          Delivers Reliable Coverage Across the Hardest Genomic Regions

          High Conversion Efficiency From Low-Input and FFPE Samples

          High-Fidelity Amplification for Low VAF Variant Detection

          Consistent Fragment Sizes Across Inputs for Scalable Workflows

          Product Data: Performance Where It Counts

          Higher Yield and Coverage From Your Most Challenging Inputs

          When working with low-input or degraded FFPE DNA, pre-capture library yield is the first indicator of whether a prep has succeeded or failed. TrueAmp consistently delivers higher pre-capture yields alongside stronger mean target coverage — without sacrificing library complexity. The result is more sequenceable molecules from the same difficult starting material, fewer failed runs, and less pressure on irreplaceable samples.

          Download Product Sheet

          Figure 1. Performance comparison of enriched libraries with low-input FFPE degraded samples (DIN <2.2) between TrueAmp Library Preparation Kit and competitor K kit, demonstrating the optimal solution for challenging sample applications. (A) The Twist TrueAmp Library Preparation Kit generates superior pre-capture library yield, indicative of high library construction and amplification efficiency. (B) The Twist TrueAmp Library Preparation Kit shows higher library complexity when compared with the competitor’s kits. This allows for more unique DNA molecules that are sequenceable in the library, reducing sequencing costs. (C) Achieves higher coverage.

          Uniform Coverage With Fewer Zero-Coverage Targets

          Gaps in coverage are not just an inconvenience — in target enrichment workflows, they represent missed variants and incomplete answers. TrueAmp reduces the proportion of zero-coverage targets across captured regions, improving coverage uniformity and giving you greater confidence that every target in your panel has been adequately interrogated. What you sequence is what you intended to sequence.

          Download Product Sheet

          Figure 2. Performance comparison of enriched libraries with low-input FFPE degraded samples (DIN <2.2) between TrueAmp Library Preparation Kit and competitor K kit, demonstrating the optimal solution for challenging sample applications. (A) Delivers excellent coverage uniformity, measured by a lower fold-80 base penalty. (B) Reduced regions with no coverage, measured by Percentage of Zero Coverage Targets.

          Consistent Performance Across GC-Extreme Regions

          GC content remains one of the most persistent sources of coverage bias in NGS library preparation. The TrueAmp Polymerase Mix is specifically formulated to maintain uniform amplification across both high- and low-GC regions — and critically, this performance holds even as PCR cycle number increases. For panels that include regulatory elements, repetitive regions, or GC-skewed targets, this translates directly to more complete and trustworthy data.

          Download Product Sheet

          Figure 3. Normalized GC bias trace showing improved coverage of the Twist TrueAmp polymerase. Libraries were prepared with Twist TrueAmp Library Preparation Kit and amplified with different polymerases and cycles.

          Upper panel: Normalized coverage against GC window plots comparing polymerases at 3 cycles of PCR.
          Lower panel: Normalized coverage against GC window plots comparing polymerases at 16 cycles of PCR.

          Reproducible Fragment Sizes Across a Wide Input Range

          Fragment size consistency underpins downstream QC, sequencing performance, and batch-to-batch reproducibility. TrueAmp delivers tightly controlled insert size distributions across a broad range of input DNA quantities, making it well-suited to mixed-input batching strategies and high-throughput workflows where uniformity at scale is non-negotiable.

          Download Product Sheet

          Figure 4. Reliable library size with Twist TrueAmp Library Prep Kit, even from ultra-low inputs.

          500 ng, 100 ng, 50 ng, 10 ng and 1 ng (gDNA) were fragmented at 32°C. 3, 5, 6, 8, 10, and 14 cycles of PCR were utilized for amplification, respectively. Samples have been performed in duplicates.

          A: Electropherograms of NGS libraries generated with the Twist TrueAmp Library Preparation Kit.

          B: Concentration of libraries after amplification for various DNA inputs.

          Tunable Insert Sizes for Application-Specific Optimisation

          Not every workflow demands the same library architecture. TrueAmp’s enzymatic fragmentation step produces repeatable, tunable insert sizes that can be adjusted to match your sequencing platform requirements and downstream analysis needs — providing flexibility without sacrificing the reproducibility your pipeline depends on.

          Download Product Sheet

          Figure 5. Tunability of Twist TrueAmp Library Prep Kit.

          A: Five electropherograms of NGS libraries generated using differing fragmentation times. 50 ng of high-quality gDNA was fragmented for various times at 32°C. 6 cycles of PCR were utilized for amplification.
          B: Median insert size vs time. 50 ng of high-quality gDNA was fragmented for various times at 32°C. Amplification was performed using 6 cycles of PCR. Samples were captured using the Twist Exome 2.0 panel.

          Watch How TrueAmp Works

          Three steps. Consistent results.

          The TrueAmp workflow is built around three precision-optimised steps that together deliver libraries you can sequence with confidence:

          Step 1 — Enzymatic Fragmentation: Extracted DNA is fragmented enzymatically to produce consistent, tunable insert sizes — no sonication required, no shear-related variability.

          Step 2 — Adapter Ligation: An optimised Twist ligase formulation maximises adapter conversion efficiency while minimising ligation bias, preserving molecular diversity from the very first step.

          Step 3 — Amplification via TrueAmp Polymerase: High-fidelity amplification boosts yield from challenging templates, maintains coverage uniformity across GC extremes, and supports sensitive downstream variant detection.

          Why It Matters to You?

          Because the Hardest Samples Carry the Most Important Questions

          In translational research and clinical genomics, the samples most critical to a study are often the ones that are hardest to sequence. Archival FFPE blocks, fine-needle aspirates, liquid biopsy specimens, and low-cellularity tumour sections are routinely degraded, limited in quantity, or variable in quality — and standard library prep kits frequently fail them.

          TrueAmp was engineered specifically for this challenge. Its impact is most pronounced where it matters most:

          Oncology and somatic variant detection

          Low VAF variants in heterogeneous tumour samples require error suppression and high complexity to call reliably. TrueAmp’s fidelity advantage directly supports this.

          FFPE-derived biobanked samples

          Legacy tissue samples carry irreplaceable longitudinal or retrospective data. Higher pre-capture yields from degraded input means more of that data becomes sequenceable.

          Target enrichment workflows

          Coverage uniformity across all captured targets — including GC-extreme regions — is the difference between a complete and an incomplete picture of your panel of interest.

          High-throughput core laboratories

           Reproducible fragment sizes and consistent performance across input ranges simplify batch QC, reduce failed libraries, and increase instrument utilisation.

          Chris Wicky

          Clinical Genomics Manager - ANZ & Country Manager - NZ

          Working with FFPE, low-input, or otherwise challenging samples?
           
          Our team can help you assess whether TrueAmp fits your current workflow and what to expect from your first run.
          Get In Touch Today

          Related Products

          Twist PCR-Free WGS Library Preparation Kit

          Bias-free whole genome libraries from high-quality input DNA, no amplification required

          View to Know More >

          Twist Custom NGS Panels

          Design and order target enrichment panels tailored to your gene list or genomic region of interest

          View to Know More >

          Twist Exome 2.0

          Comprehensive exome capture panel with proven uniformity across canonical and difficult targets

          View to Know More >

          Resources

          Twist TrueAmp Library Preparation Protocol with Twist Universal Adapter System

          Twist TrueAmp Library Preparation Kit With Twist UMI Adapter System Protocol

          Download TrueAmp Library Prep Product Sheet

          Unlock with quick sign up!

            TrueAmp Library Prep Product Sheet

            FAQs

            TrueAmp is specifically validated for challenging input types including FFPE-derived DNA (DIN <2.2), low-input samples, and variable-quality clinical specimens. It also performs well with high-quality genomic DNA across a wide input range.

            Input requirements vary by sample type and downstream application. TrueAmp is designed to deliver high conversion efficiency even from low-input and degraded samples — contact Decode Science for guidance specific to your sample type and target enrichment panel.

            Yes. TrueAmp is validated for use with both the Twist Universal Adapter System and the Twist UMI Adapter System, supporting error correction workflows for somatic variant detection and liquid biopsy applications.

            Absolutely. TrueAmp is designed to integrate with the full Twist target enrichment ecosystem, including Twist Custom NGS Panels and standard off-the-shelf panels such as Twist Exome 2.0.

            In head-to-head benchmarking against a leading competitor kit (Competitor K), TrueAmp generated superior pre-capture library yields, higher library complexity, and greater mean target coverage from the same degraded FFPE input — preserving more sequenceable molecules from precious, limited-quantity samples.

            TrueAmp libraries are compatible with Illumina sequencing platforms. For platform-specific guidance, contact our team.

            Protocols for TrueAmp with both the Universal Adapter System and UMI Adapter System are available from Decode Science on request.

            Talk to Us About TrueAmp

            We only need these information to serve you better. Decode Science respects your privacy and will never spam you with unrelated content.



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              CNV Backbone Spike-in Panels

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              Twist CNV Backbone Spike-in Panel with exome for cytogenetic research
              Stronger CNV Detection — Without Changing Your Exome Workflow.

              Large copy number variations can be easy to miss when exome sequencing focuses on exonic regions – leading to blind spots, repeat testing, and extra time interpreting unclear data. By spiking in Twist’s CNV Backbone Panels, you give your exome run the evenly spaced genomic coverage it needs to reliably surface clinically relevant CNVs — especially the ones standard exomes struggle with.

              Available in 100 kb, 50 kb, and 25 kb resolutions, the CNV Backbone Panels strengthen your detection sensitivity while keeping your workflow identical. Blend it into your existing exome panel, follow your standard Twist enrichment protocol, and immediately get more confident CNV calls backed by consistent probe tiling across intergenic regions.

              But... Why These Panels?

              1. Fine-Tune CNV Resolution

              Choose from 100 kb, 50 kb, or 25 kb probe spacing to match your CNV detection needs. Strategically tiled probes in intergenic regions enhance sensitivity for even small CNVs.

              2. Seamless Workflow Integration

              Easily spike into your Twist Exome 2.0 panel and follow standard enrichment protocols—no workflow disruption, no extra training required.

              3. Evidence-Based Performance

              Validated with highly characterized samples, the panels consistently improve CNV calls, including those smaller than 50 kb, boosting confidence in your results.

              4. Flexible Panel Sizes

              Available in 2-reaction (16 samples) and 12-reaction (96 samples) formats to fit both small-scale research and high-throughput lab workflows.

              Download Poster on utilization of these panels with exome for cytogenetic research

              Unlock with quick sign up!

                DOWNLOAD POSTER >
                Why It Matters to You

                Reliable CNV detection isn’t just a technical metric—it directly impacts research outcomes, diagnostic accuracy, and patient care. Standard exome sequencing often misses CNVs due to uneven probe coverage, creating blind spots in your analysis. Twist CNV Backbone Spike-in Panels bridge those gaps, ensuring that even subtle copy number changes are identified, so you can make confident, data-driven decisions.

                For labs and clinicians, this means fewer follow-up tests, reduced time spent troubleshooting ambiguous results, and a smoother workflow. You can trust that your exome sequencing captures the variations that truly matter, whether for rare disease research, clinical diagnostics, or high-throughput screening.

                Moreover, the ability to choose between 25 kb, 50 kb, or 100 kb resolution gives you control over sensitivity and throughput, aligning with your project goals and patient population needs. Evidence-based validation demonstrates improved detection of CNVs—including those smaller than 50 kb—so your results aren’t just comprehensive, they’re actionable.

                By integrating these panels into your existing workflow, you enhance not only the quality of your data but also the efficiency of your lab operations, freeing time and resources for deeper analysis and patient-focused outcomes.

                Select the CNV Resolution You Need

                Select CNV Resolution You Need

                Table 1. Example data of Twist CNV Backbone Spike-in Panels. A highly characterized sample set known to contain CNVs (1) and a baseline set of 12 healthy individuals were sequenced with 2×150 reads on an Illumina NovaSeq 6000. The average number of SNVs, INDELs, and CNVs called and sequencing depth at each probe density was determined for each panel when spiked into Twist Exome 2.0 plus Comprehensive Spike-in. CNV calling was performed with a commercially available software solution (2)
                 

                (1) Coriell Institute’s CNVPANEL01 – Human CNV Reference Panel.
                (2) eVai Platform (secondary workflow), enGenome Software. 

                Related Products

                Twist Exome 2.0

                Leading exome solution covering key genetic databases with high uniformity

                Twist Standard Hybridization Reagent Kit

                Reagents for high-efficiency NGS target enrichment (TE).

                Library Preparation Enzymatic Fragmentation Kit 2.0

                Enzymatic DNA fragmentation for efficient library prep.

                Ordering
                Higher Resolution: 

                110756  –  Twist 25kb CNV Backbone Spike-in Panel, 2 Reaction kit

                110757  –  Twist 25kb CNV Backbone Spike-in Panel, 12 Reaction kit

                Intermediate Resolution:

                110758 –  Twist 50kb CNV Backbone Spike-in Panel, 2 Reaction kit

                110759 –  Twist 50kb CNV Backbone Spike-in Panel, 12 Reaction kit

                Lower Resolution: 

                110760 –  Twist 100kb CNV Backbone Spike-in Panel, 2 Reaction kit

                110761 –  Twist 100kb CNV Backbone Spike-in Panel, 12 Reaction kit

                Exome Panels

                104132 –  Twist Exome 2.0, 2 Reactions, Kit

                104134 –  Twist Exome 2.0, 12 Reactions, Kit

                104136 –  Twist Exome 2.0, 96 Reactions, Kit

                105034 –  Twist Exome 2.0 plus Comprehensive Exome Spike-in, 2 Reactions

                105035 –  Twist Exome 2.0 plus Comprehensive Exome Spike-in, 12 Reactions

                105036 –  Twist Exome 2.0 plus Comprehensive Exome Spike-in, 96 Reactions

                *For research use only 

                Chris Wicky

                Clinical Genomics Manager - ANZ
                & Country Manager - NZ

                For guidance on integrating these panels into your operations, Decode Science can provide personalised support and local assistance.
                TALK TO ME DIRECTLY
                Twist Portfolio
                Twist NGS

                NGS

                Raise confidence in variant detection with superior target enrichment solutions

                Twist Oligo Pools

                Oligo Pools

                Precision, uniformity, and flexibility for results you can trust

                Synthetic Controls

                Synthetic RNA and DNA standards for assay development

                Twist Libraries

                Libraries

                Identify more hits and streamline screening with Twist's precise Variant Libraries

                Twist Genes

                Genes

                DNA Your Way: think bigger, expand your scope, and accelerate discovery in genes

                Twist Antibody

                Antibody

                Robust solutions for Antibody Discovery and Development by Twist Bioscience

                A CRE.AI.TIVE application of AI: Engineering a more resilient global food supply

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                A CRE.AI.TIVE application of AI: Engineering a more resilient global food supply

                Unlock with quick sign up!

                  Phytoform Labs harnessed its AI-powered CRE.AI.TIVE platform to develop climate-resilient crops, with a focus on drought-tolerant tomatoes. By rapidly exploring millions of potential sequence edits, the platform identified 2,000 high-potential candidates for wet-lab validation.

                  To overcome the challenge of synthesizing complex, AT-rich sequences with homopolymers, the team partnered with Twist Bioscience. Twist’s high-fidelity oligos ensured accurate transfer of AI-designed sequences to the lab, enabling efficient MPRA screening in tomato protoplasts.

                  This AI-guided workflow validated predictions while streamlining experiments—reducing waste, saving resources, and ensuring only the most promising variants progressed to in vivo testing.

                  Case Study Highlights

                  1. AI-driven design of millions of sequence variants

                  2. Efficient identification of high-impact edits, conserving time and resources

                  3. Ensuring fidelity of AI-generated oligos for complex plant sequences

                  4. Insights on impact and future directions

                  DOWNLOAD FILE

                  Chris Wicky

                  Country Manager - NZ

                  As the official distributor in Australia and New Zealand, Decode Science makes accessing genomics solutions straightforward. Our role is to connect your lab with advanced technologies, ensuring you get the right solution for your sequencing projects—delivered locally with support when you need it.
                  Get In Touch Today

                  Site Directed Mutagenesis

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                  Mutants Synthesized. Mutagenesis Simplified.

                  Make Site-Directed Mutagenesis precise, simple, and affordable with Twist’s Mutagenesis by Synthesis. Traditional site-directed mutagenesis can be time consuming and imprecise. Site directed mutagenesis through synthesis eliminates these complexities, providing you with precisely engineered sequences tailored to your exact specifications. From protein engineering to functional genomics, incorporate precision synthesis of mutants into your workflow and focus on achieving your research goals

                  Why it matters to you:

                  Precision engineering – 

                  site-directed mutagenesis, custom DNA

                  Flexible design –

                  protein engineering, functional genomics

                  Fast turnaround –

                  Express Clonal Genes, rapid synthesis

                  Cost-effective –

                  affordable variants, scalable synthesis

                  How to Qualify

                  Product: Clonal Genes
                  Number of mutation per genes: No Limit
                  Mutation type: any number of point mutation,
                  deletions, and/or insertions*
                  Pricing: Discounts on every variant
                  (Please kindly contact your Decode Sales Rep for enquiry)

                  How it Works

                  Terms and Conditions
                  * This promo is only available to customers in the Asia Pacific region (excluding China and Japan). Pricing is subjected to changes.
                  * To enjoy the special service of site directed mutagenesis, please quote your previous order number or approach Decode Science support.
                  * Each mutation is defined as any combination of bases change within a 10 codon spread over the entire gene.
                  * Average turnaround time for Express Clonal Genes is 4 -7 business days. This time will vary based on complexity and length of the sequence. Orders placed outside of the US will incur additional delivery turnaround time. Turnaround time for Clonal Genes is subject to change based on customizations and complexity. Additional specification: 50ng – 2 ug.

                  Contact Decode Support >
                  Watch Successful Implementations

                  WEBINAR

                  Stronger, tighter, and faster: designing new protein functions

                  WEBINAR

                  Designing Enzymes for the Infinite Recycling and Upcycling of Plastic

                  WEBINAR

                  Screening Gene Libraries to Rapidly Identify Immune-recognised Virulence Genes.

                  Daina Elliott

                  Business Development Manager

                  As the authorized distributor of Twist Bioscience in Australia and New Zealand, Decode Science makes integrating Twist’s DNA synthesis solutions seamless. We connect your lab with high-throughput, high-fidelity Twist technology, enabling faster, more reliable genomic workflows—with local support and guidance every step of the way.
                  Get In Touch Today
                  Twist Portfolio
                  Twist NGS

                  NGS

                  Raise confidence in variant detection with superior target enrichment solutions

                  Twist Oligo Pools

                  Oligo Pools

                  Precision, uniformity, and flexibility for results you can trust

                  Synthetic Controls

                  Synthetic RNA and DNA standards for assay development

                  Twist Libraries

                  Libraries

                  Identify more hits and streamline screening with Twist's precise Variant Libraries

                  Twist Genes

                  Genes

                  DNA Your Way: think bigger, expand your scope, and accelerate discovery in genes

                  Twist Antibody

                  Antibody

                  Robust solutions for Antibody Discovery and Development by Twist Bioscience

                  High Throughput Antibody Production

                  /in , , /by

                  PRODUCTS

                  High Throughput Antibody Production

                  Get a Quote
                  Introducing CHO and HEK293 Antibody Production

                  Twist Bioscience helps researchers speed up antibody discovery with Express Antibody Production using CHO and HEK293 expression systems. Our high-throughput antibody production platform can generate anywhere from dozens to thousands of antibodies, enabling efficient antibody screening for binding specificity and biophysical properties.

                  By leveraging Twist’s end-to-end gene-to-protein workflow, you can eliminate common antibody production bottlenecks—saving valuable time and research costs. Starting with Twist Express Genes ensures faster production times and antibodies built entirely from your custom gene sequences.

                  Key Features

                  CHO Express Antibodies

                  1. From 13 business days*
                  2. 1 mL and 8 mL scales available
                  3. Average Yields = 640 ug
                  Start Order

                  HEK293 Express Antibodies

                  1. From 10 business days*
                  2. 1 mL and 8 mL scales available
                  3. Average = Yields 760 ug
                  Start Order
                  Why Twist for Antibody Production?

                  Customization

                  Design antibodies with optimal binding affinity, specificity, and reduced immunogenicity.

                  Speed & Efficiency

                  Rapid, sequence-perfect DNA synthesis accelerates your antibody development timeline.

                  Scalable Production

                  From small-scale studies to large-scale drug discovery, scale antibody production with ease.

                  Cutting-Edge Technology

                  Access the latest gene synthesis innovations without high costs.

                  Expert Support

                  Get 24/7 guidance from antibody design to submission.

                  You design it. We build it.
                  IgG Workflow - Antibody Production
                  What Scientists Have to Say

                  Wyatt McDonnell

                  CEO & Founding Scientist • Infinnimune

                  Using Twist’s solutions saves us hundreds of thousands of dollars annually in labor costs. The cost efficiency of Twist Express Antibodies allows us to test four times as many antibodies each year as we otherwise could.

                  Dr. Ahuva Nissim

                  Professor in Antibody and Therapeutic Engineering • Queen Mary University of London

                  Working with Twist…was a great experience…[Twist’s] support was amazing. We have performed the first validation selections which looks promising…

                  Pierre Martineau

                  Deputy Director, Cancer Research Institute of Montpellier • Founder and Scientific Advisor, iMAb

                  We have performed the first selections using the Fab and scFv libraries. No problem in both cases, we got clones and some look really promising.
                  Ready To Order?
                  Our team can help you in placing the order. Click below to get a quote and fast ordering.
                  Get a Quote

                  Have a question?

                  Get a call from your local Decode Science representative to help you find the best fit genomics products for you.

                    Or give us a call at:

                    1300 581 991

                    RNA Sequencing

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                    PRODUCTS

                    RNA Sequencing

                    Get a Quote
                    RNA Sequencing

                    Twist’s RNA sequencing workflows offer a complete NGS solution that produces uniform libraries for RNA sequencing.

                    Twist offers targeted and whole transcriptome workflows that reduce time at the bench and integrate with our current set of NGS reagents, including our target enrichment kits. Each workflow delivers high quality libraries ready for sequencing from a wide range of inputs and sample types, including translation research samples.

                    Get a Quote
                    RNA Library Prep

                    Twist offers two comprehensive RNA sequencing workflows: Targeted RNA Sequencing and Whole Transcriptome Sequencing. The Targeted RNA Sequencing workflow involves creating custom panels to focus on specific RNA transcripts, utilizing streamlined library preparation kits, unique dual indices, molecular identifiers, optimized target enrichment, and a proprietary exon-aware panel design algorithm. This allows for sensitive and efficient sequencing, even with low-quality RNA from FFPE samples.

                     

                    On the other hand, the Whole Transcriptome Sequencing workflow measures expression levels of various RNA species, including mRNA and lncRNA. It involves preparing libraries from total RNA extracted from both fresh and FFPE samples using Twist RNA Library Prep and Twist rRNA & Globin Depletion kit. This workflow enables RNA Library Preparation in less than 5 hours, providing a comprehensive view of the entire transcriptome. Two charts accompany the descriptions, detailing the targeted and whole transcriptome sequencing workflows.

                    RNA Sequencing
                    Twist Targeted RNA Sequencing Workflow
                    Twist Whole Transcriptome Sequencing Workflow
                    More Targets, Fewer Reads
                    RNA Exome

                    The collaboration of Twist RNA Exome, Twist RNA Library Prep, and Twist Target Enrichment forms a reliable toolkit for transcriptome sequencing, accommodating RNA from diverse sources, including FFPE samples. Notably, the RNA Exome enhances signal strength while requiring fewer sequencing reads, enabling the detection of low-expressing targets crucial for accurate transcriptional profiling. Its exon-aware design approach allows the identification of isoforms and junctions often overlooked in conventional methods, delivering precise and uniform sequencing reads tailored for analyzing protein-coding sequences within the human transcriptome.

                     

                    Designed exclusively for the human transcriptome, the RNA Exome targets 35.8 Mb bases, 19,708 genes, and 63,215 isoforms using sequences from Gencode and RefSeq. With over a 1.8-fold enrichment compared to whole transcriptome sequencing, it features a target enrichment approach built for RNA, incorporating an exon-aware probe design for protein coding regions, fusions, and isoforms. This design not only ensures precision but also reduces reads per sample, facilitating efficient analysis of multiple samples. The RNA Exome is versatile, compatible with FFPE samples and low RNA input. In practical tests, it demonstrates the capability to detect more targets with fewer reads across a range of RNA inputs, including FFPE and universal human reference RNA, using libraries prepared with the Twist RNA Library Prep.

                    RNA Fusion

                    The Twist Alliance CeGaT RNA Fusion Panel Kit emerges as a crucial tool in the precise detection of gene fusions, particularly impactful oncogenic driver mutations in various cancer types. Rapid and accurate detection holds significant clinical implications, guiding treatment decisions effectively. This RNA-based enrichment panel stands out by offering an enhanced targeted and sensitive approach, surpassing traditional methods.

                     

                    Curated in collaboration with CeGaT, a renowned genetic diagnostic and sequencing company in Europe, this panel encompasses 160 fusion genes associated with approximately 30 cancer types. Uniquely, it is designed not only for known gene fusions but also to unveil novel ones. The panel’s efficiency is amplified when used in conjunction with the Twist RNA Library Prep Kit, ensuring an end-to-end workflow with exceptional performance.

                     

                    With an optimized design featuring 7394 probes and a breakpoint design for 66 genes, the panel excels in covering a diverse array of cancer types. The incorporation of Twist core enrichment technology further maximizes capture efficiency, instilling confidence in RNA gene fusion detection. This panel is particularly well-suited for screening oncology samples, providing critical insights for treatment decisions or uncovering novel fusions for tumor classifications.

                     

                    *Note: The Twist Alliance CeGaT RNA Fusion Panel – 3 MB holds ISO-13485 certification, reinforcing its reliability in clinical applications.

                    Ready To Order?
                    Our team can help you in placing the order. Click below to get a quote and fast ordering.
                    Get a Quote
                    NGS Portfolio

                    Custom Panels

                    Viral Panels

                    Methylation Detection

                    RNA Sequencing

                    MRD Sequencing

                    Long Read Sequencing

                    Library Preparation

                    Other Panels

                    Have a question?

                    Get a call from your local Decode Science representative to help you find the best fit genomics products for you.

                      Or give us a call at:

                      1300 581 991

                      MRD Sequencing

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                      PRODUCTS

                      MRD Sequencing

                      Get a Quote
                      MRD Sequencing

                      Minimal Residual Disease (MRD), characterized by a small number of lingering malignant cells post-treatment, poses a recurrence risk often undetectable by standard surveillance methods. Twist Bioscience’s MRD Rapid 500 Panel addresses this challenge by utilizing circulating tumor DNA for early detection. Leveraging Twist’s silicon-based DNA synthesis platform, this scalable solution offers rapid turnaround time (as few as 6 business days) with personalized variant profiles from whole-genome or whole-exome sequencing. The MRD panels, featuring 50 to 500 probes, provide comprehensive genomic insights, compatible with Twist’s NGS library preparation and hybrid capture workflow. This facilitates early intervention and enhances personalized medicine approaches in cancer recurrence monitoring.

                      Get a Quote
                      Benefits

                      Unmatched Scalability and Speed:
                      Benefit from unparalleled scalability and efficiency as you capture variants of interest with panels ranging from 50 to 500 probes. Streamline your workflow by ordering up to 150 panels at a time, and experience the rapid shipment of panels within an impressive 6 business days.

                      Customized Panel Designs:
                      Tailor your panel designs to meet your specific needs using a variant target coordinate BED file. Improve capture performance by strategically filtering probe sequences over repetitive regions, leading to enhanced efficiency and significant cost savings on overall sequencing expenses.

                      Panel Information:
                      Leverage the advantages of a single plex for 12 tests, ensuring the detection of SNVs and small indels with high precision. Implement quality control through qPCR, and rest assured with ISO-13485 certification, signifying a commitment to reliability. Conveniently receive panels in Matrix Tubes, and benefit from designs supported against hg19 and hg38 reference genomes.

                      Lab Workflow:
                      Integrate seamlessly into your lab workflow with compatibility with Twist library preparation and hybridization workflows. Follow the recommended hybridization protocol, MRD Standard Hyb 2.0, ensuring not only efficiency but also a streamlined and harmonious laboratory process.

                      MRD Rapid 500 Panel Design
                      Ready To Order?
                      Our team can help you in placing the order. Click below to get a quote and fast ordering.
                      Get a Quote
                      NGS Portfolio

                      Custom Panels

                      Viral Panels

                      Methylation Detection

                      RNA Sequencing

                      MRD Sequencing

                      Long Read Sequencing

                      Library Preparation

                      Other Panels

                      Have a question?

                      Get a call from your local Decode Science representative to help you find the best fit genomics products for you.

                        Or give us a call at:

                        1300 581 991

                        Long Read Sequencing

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                        Long Read Sequencing Panels
                        Twist Bioscience

                        Twist Alliance Long Read Sequencing Panels combine industry-leading target enrichment chemistry with the power of long read platforms. Capture the genomic regions that matter — including medically complex, difficult-to-map genes — with superior uniformity, cost efficiency, and scalable throughput.

                        DOWNLOAD BED FILES INSTANTLY

                        🏅 Compatible with Oxford Nanopore Technologies (ONT) | PacBio

                        Target What Short Reads Can't Reach

                        Short read sequencing leaves critical genomic regions in the dark — incomplete gene assemblies, unresolved haplotypes, and missed structural variants. Twist Alliance Long Read Sequencing Panels solve this by pairing optimised probe-based target enrichment with long read sequencing platforms, enabling researchers to study complex genes with high uniformity and no coverage gaps.

                        Two pre-designed panels are available to address the highest-impact clinical and research applications:

                        1. Dark Genes Panel — targets 389 clinically significant genes (20 Mb) inaccessible to short read sequencing
                        2. Long-Read PGx Panel — targets 49 pharmacogenomics genes (2 Mb) critical to drug metabolism and patient therapeutic response

                        Both panels are compatible with Oxford Nanopore Technologies (ONT) and PacBio platforms, and are backed by protocols optimised specifically for long fragment enrichment.

                        Why Researchers Choose Twist Alliance Panels

                        Probe Performance Built for Long Reads

                        Probes are specifically optimised for long fragment capture — not retrofitted from short read designs. Achieve high on-target uniformity and consistent sequencing efficiency, even across repetitive or GC-extreme regions.

                        Coverage Where It Counts

                        Purpose-built probe designs ensure balanced coverage across difficult-to-sequence and difficult-to-map regions. Full-gene phasing is achieved for genes like GBA, with no coverage gaps — even in regions historically inaccessible by short read approaches.

                        Scale Efficiently Across Cohorts

                        Protocols are optimised for long fragment enrichment and high-throughput sequencing. Pack more samples into a single run and extend your study across large patient cohorts — without sacrificing data quality or coverage depth.

                        Accurate, Clinically Meaningful Variant Calling

                        Achieve high accuracy variant calling for SNPs, SVs, and indels. Unambiguous haplotype resolution and long-range phasing enable confident interpretation — including accurate star allele calling at CYP2D6 and complete GBA gene analysis.

                        Two Panels. Two Distinct Applications
                        Twist Alliance Dark Genes Panel

                        Many of the most clinically significant genes in the human genome are also among the hardest to sequence. Segmental duplications, pseudogenes, and high homology regions make short read sequencing incomplete or unreliable. The Dark Genes Panel is the purpose-built solution.

                        Key specifications:

                        Targets 389 genes across 20 Mb

                        Key targets include GBA, SMN1/2, CYP2D6, and other medically important dark genes

                        Full-gene phasing and no coverage gap in the GBA gene

                        Enables sequencing of genes that are difficult or impossible to fully sequence with short read technology

                        Twist Alliance Dark Genes Panel

                        Unlock Dark Genes BED File

                          Twist Alliance Dark Genes Panel BED File

                          Chris Wicky

                          Clinical Genomics Manager - ANZ & Country Manager - NZ

                          As the official distributor of Twist Bioscience in Australia and New Zealand, Decode Science is providing local access to these panel solutions with region-based technical and application support. Simply talk to me and we can discuss your research needs.

                          Get In Touch Today
                          Twist Alliance Long-Read PGx Panel

                          Drug metabolism varies significantly between individuals — often due to complex gene variants and star alleles in pharmacogenomics (PGx) genes. The Long-Read PGx Panel delivers the resolution needed for confident, clinically actionable PGx interpretation.

                          Key specifications:

                          Targets 49 pharmaco-genomics genes across 2 Mb

                          Key targets include CYP2D6, HLA-A, HLA-B — highly polymorphic and critical to therapeutic decision-making

                          Accurate star allele calling at CYP2D6

                          Supports precision medicine, oncology, and population-level PGx studies

                          Twist Alliance Long-Read PGx Panel

                          Unlock PGx BED File

                            Twist Alliance Long Read PGx Panel BED File
                            Research Areas & Use Cases

                            Twist Alliance Long Read Sequencing Panels are designed for researchers working at the intersection of genomics, clinical science, and translational medicine. Relevant applications include:

                            1. Rare and Mendelian disease research — resolve dark genes and complete variant calling in genes missed by WES or short-read panels
                            2. Pharmacogenomics — achieve actionable PGx profiling at high confidence, including CYP2D6 star allele calling for drug dosing decisions
                            3. Structural variant discovery — detect SVs and indels with long-range phasing that short reads cannot resolve
                            4. Carrier screening — comprehensive gene coverage across clinically important genes including SMN1/2 and GBA
                            5. Oncology & somatic variant analysis — high-accuracy somatic calling in difficult genomic regions
                            6. Population-scale cohort studies — cost-effective per-sample pricing and high-throughput protocols support large cohort work
                            7. HLA typing — unambiguous allele resolution for transplant and immunology applications
                            Specifications

                            To place an order or request pricing for ANZ:

                            Contact Decode Science | support@decodescience.com.au | 1300 581 991

                            Get In Touch Today
                            Technical Specification of Dark Genes vs Long Read PGx Panels

                            All Twist Alliance Long Read Sequencing Panels are available in 2, 12, and 96 reaction kit formats to support pilot studies through to large-scale cohort sequencing.

                            Order Information for long Read PGx Panels
                            Resources

                            Long-read capture with Twist target enrichment system poster

                            Long Read Library Preparation and Standard Hyb v2 Enrichment

                            On-demand webinar: Long read target enrichment — best practices and case studies

                            NGS Portfolio

                            Custom Panels

                            Viral Panels

                            Methylation Detection

                            RNA Sequencing

                            MRD Sequencing

                            Long Read Sequencing

                            Library Preparation

                            Other Panels

                            Contact Decode Science Today

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