Site Directed Mutagenesis

November 17, 2025/in Partners, Synbio Genes, Twist/by Harshita Sharma

Mutants Synthesized. Mutagenesis Simplified.

Make Site-Directed Mutagenesis precise, simple, and affordable with Twist’s Mutagenesis by Synthesis. Traditional site-directed mutagenesis can be time consuming and imprecise. Site directed mutagenesis through synthesis eliminates these complexities, providing you with precisely engineered sequences tailored to your exact specifications. From protein engineering to functional genomics, incorporate precision synthesis of mutants into your workflow and focus on achieving your research goals

Why it matters to you:

Precision engineering –

site-directed mutagenesis, custom DNA

Flexible design –

protein engineering, functional genomics

Fast turnaround –

Express Clonal Genes, rapid synthesis

Cost-effective –

affordable variants, scalable synthesis

How to Qualify

Product: Clonal Genes
Number of mutation per genes: No Limit
Mutation type: any number of point mutation,
deletions, and/or insertions*
Pricing: Discounts on every variant
(Please kindly contact your Decode Sales Rep for enquiry)

How it Works

Terms and Conditions
* This promo is only available to customers in the Asia Pacific region (excluding China and Japan). Pricing is subjected to changes.
* To enjoy the special service of site directed mutagenesis, please quote your previous order number or approach Decode Science support.
* Each mutation is defined as any combination of bases change within a 10 codon spread over the entire gene.
* Average turnaround time for Express Clonal Genes is 4 -7 business days. This time will vary based on complexity and length of the sequence. Orders placed outside of the US will incur additional delivery turnaround time. Turnaround time for Clonal Genes is subject to change based on customizations and complexity. Additional specification: 50ng – 2 ug.

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As the authorized distributor of Twist Bioscience in Australia and New Zealand, Decode Science makes integrating Twist’s DNA synthesis solutions seamless. We connect your lab with high-throughput, high-fidelity Twist technology, enabling faster, more reliable genomic workflows—with local support and guidance every step of the way.

Twist Portfolio

Clonal Genes

January 16, 2024/in Partners, Synbio Genes, Twist/by Harshita Sharma

PRODUCTS

Clonal Genes

Overview

Twist Bioscience’s advanced platform is designed to meet diverse DNA synthesis needs, with the capability to synthesize hundreds of thousands of genes each month. Utilizing a silicon-based platform for DNA synthesis, Twist delivers highly precise, sequence-perfect clonal genes of various lengths and complexities, all verified through Next-Generation Sequencing (NGS). Now, with the introduction of the Express Genes service, Twist offers even faster turnaround times, synthesising NGS-verified Clonal Genes in as few as 5 days.

Twist Bioscience’s Express Genes service offers researchers a cost-effective, customizable, and scalable solution for obtaining high-quality clonal genes with efficient turnaround times. This service empowers researchers to accelerate their projects and advance their scientific endeavors with confidence.

Low Cost – High Quality:

Priced from 18¢ (AUD) per base pair.

No hidden sub-cloning or DNA complexity fees.

Delivery in as fast as 12 business days.

DNA Your Way:

Customize 0.3 – 5 kb genes cloned into a plasmid of your choice.

Choose from Twist Catalog Vectors or provide your own.

Four preparation scales (50ng – 2µg | 2µg – 10µg | 10µg – 100µg | 100µg – 1mg).

Options for normalization and endotoxin-free available.

Scalable Synthesis:

No order limits, providing flexibility.

Same turnaround time regardless of order size.

Express Genes Service

Introducing Twist Bioscience’s Express Genes service*— synthesising NGS-verified, sequence-perfect Clonal Genes with an unprecedented order-to-ship turnaround time of 5-7 business days. Explore our full Clonal Genes offering below and experience the speed and efficiency of Express Genes for your research needs.

*Terms and Conditions: Eligible Express Genes ship in 5-7 business days. This time will vary based on complexity and length of the sequence. Orders placed outside of the US will incur additional delivery turnaround time. Turnaround time for Clonal Genes is subject to change based on customizations and complexity. Average turnaround time for Clonal Genes is 10-15 business days. New vector onboarding for both Express Genes and Clonal Genes will add additional time.

DATA

The data presented illustrates the high quality and precision of Twist Bioscience’s Clonal Genes, showcasing a graphical representation of the standard Next-Generation Sequencing (NGS) verification performed on each clonal gene. The featured clonal gene in the figure serves as an example of an error-free clone. The read depth is indicated for the entire plasmid, and no Single Nucleotide Polymorphisms (SNPs) or insertions/deletions (indels) were detected, emphasizing the accuracy and reliability of Twist’s clonal gene synthesis.

This data emphasizes Twist’s commitment to providing researchers with sequence-perfect clonal genes, ensuring high-quality and error-free results for a wide range of applications in the field of genetic research and synthetic biology.

As the official distributor for Twist Bioscience in Australia and New Zealand, Decode Science provides easy access to Twist Clonal Genes —high-quality, scalable DNA synthesis solutions for genomics research.

Instant Download the Product Sheet for Clonal Genes

Twist Bioscience is revolutionizing gene synthesis, a cornerstone of synthetic and molecular biology. Our silicon-based DNA writing platform boosts throughput and scalability while cutting turnaround times and cost per base.

Inside this Product Sheet:

Clonal Gene Synthesis Specifications
Key Considerations for Clonal Gene Synthesis
Ordering Process for Clonal Gene Synthesis

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Finally, an easy way to order DNA

FAQs

What determines the maximum number of oligos in a pool?

The number of unique oligos per pool depends on your experimental needs. Twist’s silicon-based synthesis platform supports pools ranging from hundreds to over a million sequences without sacrificing uniformity.

Can oligo sequences be designed for complex genomic targets?

Yes. Twist Oligo Pools can be customized for highly specific targets, including CRISPR libraries, mutagenesis studies, hybrid capture, or any sequence requiring precise coverage.

How consistent is oligo representation across a pool?

Twist uses NGS-based quality control to ensure even representation and uniform synthesis of all sequences, minimizing bias and dropout in downstream applications.

Are oligo pools compatible with high-throughput workflows?

Absolutely. Pools are synthesized to be fully compatible with automated workflows and sequencing pipelines, making them scalable from small experiments to genome-wide screens.

How is synthesis accuracy verified?

Each pool undergoes sequencing analysis to confirm that all intended sequences are present and correctly synthesized, ensuring reliable and reproducible experimental results.

Have another question before ordering?

Reach out to the Decode Science team—we’re ready to provide instant guidance and support.

As the official Twist Bioscience distributor in Australia and New Zealand, Decode Science connects your lab with Twist’s advanced synthesis platform, making it easy to design, order, and receive high-quality oligo pools with reliable local support.

NGS

SynBio

Ready To Order?

Our team can help you in placing the order. Click below to get a quote and fast ordering.

Synbio Genes Portfolio

Gene Fragments

Clonal Genes

Have a question?

Get a call from your local Decode Science representative to help you find the best fit genomics products for you.

Or give us a call at:

1300 581 991

Gene Fragments

January 16, 2024/in Partners, Synbio Genes, Twist/by Harshita Sharma

PRODUCTS

Gene Fragments

Overview

Twist Bioscience offers Synthetic Gene Fragments as a fast, economical, and efficient solution for building genes in your research. These gene fragments enhance the cloning process by minimizing the need for colony screening, saving valuable time and reducing overall costs associated with cloning and sequencing.

By leveraging Twist’s Synthetic Gene Fragments, researchers can think bigger, design on a grander scale, and accelerate their discoveries. With the advantage of an industry-leading error rate, customizable lengths and yields, and the convenience of no order limits, Twist’s Gene Fragments offer a cost-effective and time-efficient solution for diverse research needs. To learn more or place an order, researchers can explore the product sheet or directly proceed with ordering from Twist Bioscience.

Fast and Economical:

Gene Fragments for assembly and cloning.
Compatible with all downstream cloning methods.
Priced from 13¢ (AUD) per base pair.
Synthesised in as little as 2 business days.

Screen Less, Discover More:

Industry-leading error rate of 1:7500.
Length: 300 bp – 1800 bp.
Yield: 100 ng – 1 µg.
No order limit.

Twist Bioscience’s DNA synthesis technology stands out by outperforming competitors with exceptionally low error rates. In a direct comparison of Gene Fragment products, Twist consistently demonstrated the lowest error rate, as illustrated in the graph comparing Twist and Integrated DNA Technologies, Inc. (IDT). The results reveal that Twist Gene Fragments exhibit greater sequence accuracy compared to eBlocks and gBlocks, boasting an average of 2-fold greater accuracy in sequence fidelity over gBlocks.

Moreover, Twist Gene Fragments consistently yield the highest percentage of perfect clones, offering a significant advantage in terms of time and cost savings for researchers. The graph showcasing a direct comparison of the percentage of sequence perfect clones across various gene lengths and sequences for three different gene products highlights Twist’s superiority. The data, derived from a set of 63 sequences with diverse gene lengths, reflects the broad applicability of Twist’s technology in addressing the varied requirements of real-world synthetic biology applications.

Ready To Order?

Our team can help you in placing the order. Click below to get a quote and fast ordering.

SynBio Genes Portfolio

Gene Fragments

Clonal Genes

Have a question?

Get a call from your local Decode Science representative to help you find the best fit genomics products for you.

Or give us a call at:

1300 581 991

Archive for category: Synbio Genes

Site Directed Mutagenesis

Daina Elliott

NGS

Oligo Pools

Synthetic Controls

Libraries

Genes

Antibody

Clonal Genes