Proteomics in Transition: From Discovery to Diagnostic Relevance Whitepaper

November 24, 2025/in Quanterix/by Harshita Sharma

Proteomics in Transition: From Discovery to Diagnostic Relevance Whitepaper

For years, discovery proteomics uncovered hundreds of candidate biomarkers — but most stalled before reaching the clinic. New digital platforms change that: they deliver femtogram-level sensitivity, reproducible quantification across sites, and the analytical rigor required for regulatory and clinical use.

Why this matters
Proteins reflect real-time biology. That means faster detection, better trial enrollment, and clearer measures of therapeutic effect. Whether you’re developing a diagnostic, designing an adaptive trial, or building a multi-omics model, the right proteomic data reduces guesswork and accelerates decisions.

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As the official distributor in Australia and New Zealand, Decode Science makes accessing genomics solutions straightforward. Our role is to connect your lab with advanced technologies, ensuring you get the right solution for your sequencing projects—delivered locally with support when you need it.

BCR Sequencing of 1 Million Healthy and Diseased Samples in a Single Experiment

November 24, 2025/in Parse/by Harshita Sharma

BCR Sequencing of 1 Million Healthy and Diseased Samples in a Single Experiment

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Key Takeaways

Profiled 1 million human B cells in a single experiment
Detected 900,000+ unique paired clonotypes across Type 1 Diabetes, Multiple Sclerosis, Rheumatoid Arthritis, Crohn’s, Celiac, and healthy donors
Achieved sensitive detection of CDR3 regions, clonotypes, and full-length sequences at scale

Evercode BCR uncovered over 900,000 unique paired clonotypes across 24 samples in a single experiment. Negatively selected B cells from 12 healthy donors were purchased, while pan B cells were isolated from 12 autoimmune-diseased human PBMCs and fixed using the Evercode Cell Fixation Kit v3 to preserve cell structure and protect RNA integrity. Fixed samples were stored at -80°C until all were ready for combined processing with the Evercode BCR Mega Kit. Whole transcriptome and BCR-specific libraries were sequenced on the Illumina Novaseq X, and data were analyzed using Parse Biosciences’ Analysis Pipeline v1.3.0. Clustering with Seurat 5.0 showed that the majority of cells corresponded to major B cell subtypes, as illustrated in the UMAP below (Figure 1).

The assay demonstrated high sensitivity, detecting paired heavy and light chains in up to 89% of cells (Figure 2).

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As the official distributor in Australia and New Zealand, Decode Science makes accessing genomics solutions straightforward. Our role is to connect your lab with advanced technologies, ensuring you get the right solution for your sequencing projects—delivered locally with support when you need it.

MosaiX High-Complexity Library Prep Kit – Early Access Promotion

November 24, 2025/in SeqWell/by Harshita Sharma

MosaiX High-Complexity Library Prep Kit ,
– Early Access Promotion

Be among the first to experience the newest in high-complexity library prep technology.

SeqWell has just launched the MosaiX High-Complexity Library Prep Kit, unveiled at ASHG, and we’re offering a limited-time early-access promotion for researchers in AU & NZ.

5/5

Early-Access Offer Includes:

How to Qualify?

CNV Backbone Spike-in Panels

November 19, 2025/in NGS, Partners, Twist/by Harshita Sharma

Stronger CNV Detection — Without Changing Your Exome Workflow.

Large copy number variations can be easy to miss when exome sequencing focuses on exonic regions – leading to blind spots, repeat testing, and extra time interpreting unclear data. By spiking in Twist’s CNV Backbone Panels, you give your exome run the evenly spaced genomic coverage it needs to reliably surface clinically relevant CNVs — especially the ones standard exomes struggle with.

Available in 100 kb, 50 kb, and 25 kb resolutions, the CNV Backbone Panels strengthen your detection sensitivity while keeping your workflow identical. Blend it into your existing exome panel, follow your standard Twist enrichment protocol, and immediately get more confident CNV calls backed by consistent probe tiling across intergenic regions.

But... Why These Panels?

Download Poster on utilization of these panels with exome for cytogenetic research

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Why It Matters to You

Reliable CNV detection isn’t just a technical metric—it directly impacts research outcomes, diagnostic accuracy, and patient care. Standard exome sequencing often misses CNVs due to uneven probe coverage, creating blind spots in your analysis. Twist CNV Backbone Spike-in Panels bridge those gaps, ensuring that even subtle copy number changes are identified, so you can make confident, data-driven decisions.

For labs and clinicians, this means fewer follow-up tests, reduced time spent troubleshooting ambiguous results, and a smoother workflow. You can trust that your exome sequencing captures the variations that truly matter, whether for rare disease research, clinical diagnostics, or high-throughput screening.

Moreover, the ability to choose between 25 kb, 50 kb, or 100 kb resolution gives you control over sensitivity and throughput, aligning with your project goals and patient population needs. Evidence-based validation demonstrates improved detection of CNVs—including those smaller than 50 kb—so your results aren’t just comprehensive, they’re actionable.

By integrating these panels into your existing workflow, you enhance not only the quality of your data but also the efficiency of your lab operations, freeing time and resources for deeper analysis and patient-focused outcomes.

Select the CNV Resolution You Need

Table 1. Example data of Twist CNV Backbone Spike-in Panels. A highly characterized sample set known to contain CNVs (1) and a baseline set of 12 healthy individuals were sequenced with 2×150 reads on an Illumina NovaSeq 6000. The average number of SNVs, INDELs, and CNVs called and sequencing depth at each probe density was determined for each panel when spiked into Twist Exome 2.0 plus Comprehensive Spike-in. CNV calling was performed with a commercially available software solution (2)

(1) Coriell Institute’s CNVPANEL01 – Human CNV Reference Panel.
(2) eVai Platform (secondary workflow), enGenome Software.

A CRE.AI.TIVE application of AI: Engineering a more resilient global food supply

November 17, 2025/in Twist/by Harshita Sharma

A CRE.AI.TIVE application of AI: Engineering a more resilient global food supply

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Phytoform Labs harnessed its AI-powered CRE.AI.TIVE platform to develop climate-resilient crops, with a focus on drought-tolerant tomatoes. By rapidly exploring millions of potential sequence edits, the platform identified 2,000 high-potential candidates for wet-lab validation.

To overcome the challenge of synthesizing complex, AT-rich sequences with homopolymers, the team partnered with Twist Bioscience. Twist’s high-fidelity oligos ensured accurate transfer of AI-designed sequences to the lab, enabling efficient MPRA screening in tomato protoplasts.

This AI-guided workflow validated predictions while streamlining experiments—reducing waste, saving resources, and ensuring only the most promising variants progressed to in vivo testing.

Case Study Highlights

AI-driven design of millions of sequence variants
Efficient identification of high-impact edits, conserving time and resources
Ensuring fidelity of AI-generated oligos for complex plant sequences
Insights on impact and future directions

As the official distributor in Australia and New Zealand, Decode Science makes accessing genomics solutions straightforward. Our role is to connect your lab with advanced technologies, ensuring you get the right solution for your sequencing projects—delivered locally with support when you need it.

Modified LongPlex™ Protocol (LongPlex XL)

November 17, 2025/in SeqWell/by Harshita Sharma

Modified LongPlex™ Protocol (LongPlex XL)

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This technical note outlines an alternative workflow for generating 10–15 kb HiFi reads from high-quality genomic DNA using the LongPlex Long Fragment Multiplexing Kit. In this approach, LongPlex is used to fragment and barcode samples, and PacBio’s Short Read Eliminator (SRE) is applied to size-select fragments >10 kb before SMRTbell® library preparation.

LongPlex uses plate-based transposase tagmentation for multiplexed fragmentation and barcoding, removing the need for mechanical shearing and allowing barcoded samples to be pooled before SMRTbell prep. This simplifies the workflow, increases throughput, and lowers library prep costs.

The standard LongPlex protocol generates 6–9 kb HiFi reads from high- to medium-quality DNA—ideal for microbial and other small-genome projects. However, users working with higher-quality DNA may want longer HiFi reads to maximize gigabase yield on PacBio systems.

This modified workflow is only suitable for high-quality DNA (Femto Pulse GQN30kb ≥7). Using degraded DNA will result in substantial sample loss during SRE size selection.

As the official distributor in Australia and New Zealand, Decode Science makes accessing genomics solutions straightforward. Our role is to connect your lab with advanced technologies, ensuring you get the right solution for your sequencing projects—delivered locally with support when you need it.

Site Directed Mutagenesis

November 17, 2025/in Partners, Synbio Genes, Twist/by Harshita Sharma

Mutants Synthesized. Mutagenesis Simplified.

Make Site-Directed Mutagenesis precise, simple, and affordable with Twist’s Mutagenesis by Synthesis. Traditional site-directed mutagenesis can be time consuming and imprecise. Site directed mutagenesis through synthesis eliminates these complexities, providing you with precisely engineered sequences tailored to your exact specifications. From protein engineering to functional genomics, incorporate precision synthesis of mutants into your workflow and focus on achieving your research goals

Why it matters to you:

Precision engineering –

site-directed mutagenesis, custom DNA

Flexible design –

protein engineering, functional genomics

Fast turnaround –

Express Clonal Genes, rapid synthesis

Cost-effective –

affordable variants, scalable synthesis

How to Qualify

Product: Clonal Genes
Number of mutation per genes: No Limit
Mutation type: any number of point mutation,
deletions, and/or insertions*
Pricing: Discounts on every variant
(Please kindly contact your Decode Sales Rep for enquiry)

How it Works

Terms and Conditions
* This promo is only available to customers in the Asia Pacific region (excluding China and Japan). Pricing is subjected to changes.
* To enjoy the special service of site directed mutagenesis, please quote your previous order number or approach Decode Science support.
* Each mutation is defined as any combination of bases change within a 10 codon spread over the entire gene.
* Average turnaround time for Express Clonal Genes is 4 -7 business days. This time will vary based on complexity and length of the sequence. Orders placed outside of the US will incur additional delivery turnaround time. Turnaround time for Clonal Genes is subject to change based on customizations and complexity. Additional specification: 50ng – 2 ug.

Watch Successful Implementations

WEBINAR

Stronger, tighter, and faster: designing new protein functions

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Designing Enzymes for the Infinite Recycling and Upcycling of Plastic

WEBINAR

Screening Gene Libraries to Rapidly Identify Immune-recognised Virulence Genes.

As the authorized distributor of Twist Bioscience in Australia and New Zealand, Decode Science makes integrating Twist’s DNA synthesis solutions seamless. We connect your lab with high-throughput, high-fidelity Twist technology, enabling faster, more reliable genomic workflows—with local support and guidance every step of the way.

Twist Portfolio

Comparison of Evercode™ WT v3 and Chromium™ GEM-X Single Cell 3’ Kit v4 in Mouse Brain Nuclei

November 13, 2025/in Parse/by Harshita Sharma

Comparison of Evercode™ WT v3 and Chromium™ GEM-X Single Cell 3’ Kit v4 in Mouse Brain Nuclei

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Comparison Highlights

Evercode WT v3 delivers superior gene detection in head-to-head sensitivity tests.
Cell type proportions remain consistently represented.
Analysis of differential gene expression shows Evercode WT v3 identifies 2× more genes than competing methods.

Study Overview
Two embryonic mouse brains were sagittally dissected and flash-frozen by a third-party vendor.
One half from each brain was processed by a 10x Genomics certified provider for nuclei isolation and library preparation, while the other halves were processed by Parse Biosciences using their own workflow.
Sequencing was performed by a third-party, and data analysis was completed using each manufacturer’s respective pipeline.

As the official distributor in Australia and New Zealand, Decode Science makes accessing genomics solutions straightforward. Our role is to connect your lab with advanced technologies, ensuring you get the right solution for your sequencing projects—delivered locally with support when you need it.

Optimized CRISPR/Cas9 Gene Knockout PDF

November 13, 2025/in EditCo, Partners/by Harshita Sharma

Accelerate knockout experiments with XDel’s next-generation CRISPR design.

EditCo Bio’s XDel technology eliminates the need for guide RNA pre-screening, using a coordinated multi-gRNA design that delivers consistently high on-target editing across immortalized, primary, and iPSC lines. Validated through 768 edited samples and 4,816 NGS libraries, XDel achieves robust knockout efficiency while minimizing off-target effects—saving time and improving reproducibility across diverse cell types.

Validated performance. Proven precision.
With a standardized amplicon sequencing QC workflow and high-throughput automation, XDel enables scalable, high-confidence Cas9-mediated editing for both pooled and single-cell clone analysis. Download the full guide to explore the data, workflows, and results behind EditCo Bio’s high-efficiency gene knockout strategy.

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As the official distributor in Australia and New Zealand, Decode Science makes accessing genomics solutions straightforward. Our role is to connect your lab with advanced technologies, ensuring you get the right solution for your sequencing projects—delivered locally with support when you need it.

Excel Sheet: STOmics Validated Tissue List

October 30, 2025/in STOmics/by Harshita Sharma

STOmics Validated Tissue List

Our STOmics validated tissue list provides researchers with a comprehensive reference of hundreds of tissue types successfully tested using Stereo-seq, the cutting-edge spatial transcriptomics technology. Each tissue entry includes detailed sample information, experimental parameters, and test results, allowing scientists to make informed decisions before starting their single-cell or spatial transcriptomics experiments. By consulting this list, you can ensure compatibility with your tissue samples and streamline your experimental design.

The list not only highlights tissue types that have been validated but also provides insights into the experimental conditions that yielded the most reliable results. Researchers can leverage this information to optimize sample preparation, sequencing protocols, and data quality control measures. This reduces trial-and-error, saves valuable time and resources, and ensures reproducibility across studies. It is an essential tool for anyone planning to use Stereo-seq for spatial gene expression profiling.

In addition, our STOmics validated tissue list supports better planning for large-scale studies and comparative analyses. By providing a centralized reference for tissue performance, it helps guide tissue selection, anticipate potential challenges, and maximize experimental success. Whether you are exploring new tissue types or scaling up existing workflows, this validated tissue list is your key resource for robust, high-quality spatial transcriptomics research.

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Notices:

The STOmics validated tissue list was generated using standard tissue and sample types, all of which are frozen. Each tissue sample had an area of less than 1 cm² and was sectioned at a thickness of 10 μm. Most experiments were performed using the Stereo-seq Transcriptomics Kit V1.2, with a few using V1.1. Sequencing depth ranged from 1–3 G reads per sample, and data were processed using the Stereo-seq Analysis Workflow (SAW) versions V2.1.0–V5.1.3. Testing was conducted between 2020 and 2022.

Please note that all test parameters and results are highly dependent on the tissue and sample type. This information should be used as a reference guide to help design and optimize your own experiments, rather than as definitive outcomes for all samples.

Key parameters included in the list:

MID (K): Bin200_Median_MID in thousands
GENETYPE (K): Bin200_Median_Genetype in thousands

As the official distributor in Australia and New Zealand, Decode Science makes accessing genomics solutions straightforward. Our role is to connect your lab with advanced technologies, ensuring you get the right solution for your sequencing projects—delivered locally with support when you need it.

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Dr. Ebru Boslem

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Dr. Ebru Boslem

MosaiX High-Complexity Library Prep Kit , – Early Access Promotion

5/5

Chris Wicky

Early-Access Offer Includes:

How to Qualify?

Complete a short feedback survey by 31 December to access the early-bird discounted rate.

1. Fine-Tune CNV Resolution

2. Seamless Workflow Integration

3. Evidence-Based Performance

4. Flexible Panel Sizes

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Chris Wicky

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Chris Wicky

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Chris Wicky

Daina Elliott

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Dr. Ebru Boslem

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Hamza Hassan

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Dr. Ebru Boslem

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Special October Offer

FREE UMBRELLA

MosaiX High-Complexity Library Prep Kit ,
– Early Access Promotion