Entries by Harshita Sharma

Proteomics in Transition: From Discovery to Diagnostic Relevance Whitepaper

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Proteomics in Transition: From Discovery to Diagnostic Relevance Whitepaper

For years, discovery proteomics uncovered hundreds of candidate biomarkers — but most stalled before reaching the clinic. New digital platforms change that: they deliver femtogram-level sensitivity, reproducible quantification across sites, and the analytical rigor required for regulatory and clinical use.

Why this matters
Proteins reflect real-time biology. That means faster detection, better trial enrollment, and clearer measures of therapeutic effect. Whether you’re developing a diagnostic, designing an adaptive trial, or building a multi-omics model, the right proteomic data reduces guesswork and accelerates decisions.

Quanterix Proteomics in Transition

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    Dr. Ebru Boslem

    ANZ Market Manager - Research Genomics

    As the official distributor in Australia and New Zealand, Decode Science makes accessing genomics solutions straightforward. Our role is to connect your lab with advanced technologies, ensuring you get the right solution for your sequencing projects—delivered locally with support when you need it.



    Get In Touch Today

    BCR Sequencing of 1 Million Healthy and Diseased Samples in a Single Experiment

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    BCR Sequencing of 1 Million Healthy and Diseased Samples in a Single Experiment

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      Key Takeaways

      1. Profiled 1 million human B cells in a single experiment

      2. Detected 900,000+ unique paired clonotypes across Type 1 Diabetes, Multiple Sclerosis, Rheumatoid Arthritis, Crohn’s, Celiac, and healthy donors

      3. Achieved sensitive detection of CDR3 regions, clonotypes, and full-length sequences at scale

      Evercode BCR uncovered over 900,000 unique paired clonotypes across 24 samples in a single experiment. Negatively selected B cells from 12 healthy donors were purchased, while pan B cells were isolated from 12 autoimmune-diseased human PBMCs and fixed using the Evercode Cell Fixation Kit v3 to preserve cell structure and protect RNA integrity. Fixed samples were stored at -80°C until all were ready for combined processing with the Evercode BCR Mega Kit. Whole transcriptome and BCR-specific libraries were sequenced on the Illumina Novaseq X, and data were analyzed using Parse Biosciences’ Analysis Pipeline v1.3.0. Clustering with Seurat 5.0 showed that the majority of cells corresponded to major B cell subtypes, as illustrated in the UMAP below (Figure 1).

      The assay demonstrated high sensitivity, detecting paired heavy and light chains in up to 89% of cells (Figure 2).

      Downloads



      All Sample Summary Report



      WT: All Genes (CSV)



      WT: Cell Metadata (CSV)



      WT: Count Matrix (23.3 GB)



      BCR: Barcode Report (TSV)



      BCR: Clonotype Frequency (TSV)



      BCR: The Adaptive Immune Receptor Repertoire (AIRR) File (3.7 GB)

      Dr. Ebru Boslem

      ANZ Market Manager - Research Genomics

      As the official distributor in Australia and New Zealand, Decode Science makes accessing genomics solutions straightforward. Our role is to connect your lab with advanced technologies, ensuring you get the right solution for your sequencing projects—delivered locally with support when you need it.



      Get In Touch Today

      Validation Services

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      NGS Validation Package
      – Expert Support for Your Lab

      Get your lab NGS-ready with our comprehensive Validation Package, designed for labs with limited experience, staff, or analytical expertise. Our modular approach ensures tailored support for your lab’s needs.

      Chris Wicky

      Clinical Sales Manager - ANZ & Country Manager - NZ



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      Scott Coutts

      Clinical Field Application Scientist



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      Ain Roesley

      Clinical Field Application Scientist



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      But... Why Do You Need NGS Validation Support?

      1. Accreditation Will Expose Weak Assumptions

      Assessors don’t care that your assay “works in practice.” They care about documented evidence, traceability, and defensible decisions. Validation gaps get found fast and fixing them after assessment is expensive.

      2. NGS Failure Modes Are Not Obvious

      NGS doesn’t fail loudly. It fails silently coverage bias, pipeline misconfiguration, poor QC thresholds, or incorrect variant filtering. Without structured validation, you won’t know what you’re missing until it’s too late.

      3. Internal Teams Are Already Stretched

      Most labs underestimate the time, expertise, and coordination required for end-to-end validation. This package prevents validation from becoming a six-month distraction that stalls go-live timelines.

      4. You Need Evidence You Can Defend

      Validation isn’t about generating data. It’s about generating defensible conclusions. We help you prove performance, define limitations, and document decisions in a way that stands up to scrutiny.

      Choose Your Preferred Module

      Module 1 - Consultancy Support



      • Accreditation consultancy


      • Guidance on NATA requirements, bioinformatic pipelines, project management, and planning


      • Estimated Time: From 6 hours of face-to-face consultation (including documentation & evaluation)


      • Deliverables: Comprehensive Accreditation Plan



      Select Module 1

      Wet Lab Validation Support Services

      Module 2 - Wet Lab Validation Support



      • Preparation of samples (from extracted DNA), libraries, QC, sequencing


      • Full report for up to 96 samples


      • Options for automated or manual preparation


      • Training session for your team to run samples and generate additional validation data


      • Estimated Time: From 2 weeks (including documentation & evaluation)

        Breaks into phases requiring initial input from your team



      Select Module 2

      Dry Lab Validation Support Services Decode Science

      Module 3 - Dry Lab Validation Support



      • Process raw sequencing data or VCF/BAM files


      • Establish secondary analysis pipeline if needed (in collaboration with Franklin)


      • Initial high-level analysis to check concordance and troubleshoot issues


      • Team training session to run cases and generate additional validation data


      • Estimated Time: From 2 weeks (including documentation & evaluation)

        Phased approach requiring starting data from your team



      Select module 3

      Proud Partnerships

      Twist Bioscience
      MGI
      seqwell-logo-540
      Franklin by Qiagen

      How does it work?

      1

      Initiation

      You reach out to Decode Science with your preferred module selection

      2

      Assessment Phase

      Decode team assess your clinical needs

      3

      Support Plan Prep

      Decode team builds a validation support plan

      4

      We Work Together

      We work with you to implement the plan. Work with you to prepare for validation.

      we’re here to all your questions

      More than zero — less than doing it alone. Expect defined input points (samples, data, decisions), but not weeks of unfocused effort. If your team can’t commit any time, validation will fail regardless of vendor.

      We align validation activities to accreditation expectations (e.g. NATA), but we don’t “guarantee” accreditation. Anyone who does is lying. What we do guarantee is that your validation is structured, documented, and assessor-ready.

      Yes. The framework applies to new panels, new instruments, chemistry changes, pipeline updates, and assay expansions. If performance characteristics change, validation is not optional.

      They will learn. Each module includes hands-on training so your team can independently generate additional validation data after we step away. If knowledge transfer doesn’t happen, the engagement has failed.

      You retain all documentation, workflows, and training outputs. No vendor lock-in. If assessors raise questions later, you’ll have the evidence to respond — without scrambling.

      Decode Science team
      is well equipped to help you out.

      Our team is one form away.

      We only need below information to serve you better. Decode Science respects your privacy and will never spam you with unrelated content.





        Decode Science is committed to protecting and respecting your privacy, and we’ll only use your personal information to administer your account and to provide the products and services you requested from us.

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        You can unsubscribe from these communications at any time.

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        MosaiX High-Complexity Library Prep Kit – Early Access Promotion

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        MosaiX High-Complexity Library Prep Kit ,
        – Early Access Promotion

        Be among the first to experience the newest in high-complexity library prep technology.

        SeqWell has just launched the MosaiX High-Complexity Library Prep Kit, unveiled at ASHG, and we’re offering a limited-time early-access promotion for researchers in AU & NZ.



        Place Your Order Today >



        5/5

        Chris Wicky

        Clinical Sales Manager - ANZ & Country Manager - NZ



        View Full Profile

        MosaiX-technical-workflow-768x547


        Early-Access Offer Includes:



        • Introductory Discount on MosaiX library prep


        • UDI Primers Bundled at no extra cost


        • Access to MosaiX’s latest chemistry for high-complexity, uniform libraries


        • Chance to provide feedback and help shape future developments


        How to Qualify?

        Complete a short feedback survey by 31 December to access the early-bird discounted rate.

        Interested?
        Click below or contact us to get the exact AU/NZ discounted rates and place your order.



        Talk to Chris Wicky >

        CNV Backbone Spike-in Panels

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        Twist CNV Backbone Spike-in Panel with exome for cytogenetic research

        Stronger CNV Detection — Without Changing Your Exome Workflow.

        Large copy number variations can be easy to miss when exome sequencing focuses on exonic regions – leading to blind spots, repeat testing, and extra time interpreting unclear data. By spiking in Twist’s CNV Backbone Panels, you give your exome run the evenly spaced genomic coverage it needs to reliably surface clinically relevant CNVs — especially the ones standard exomes struggle with.

        Available in 100 kb, 50 kb, and 25 kb resolutions, the CNV Backbone Panels strengthen your detection sensitivity while keeping your workflow identical. Blend it into your existing exome panel, follow your standard Twist enrichment protocol, and immediately get more confident CNV calls backed by consistent probe tiling across intergenic regions.

        But... Why These Panels?

        1. Fine-Tune CNV Resolution

        Choose from 100 kb, 50 kb, or 25 kb probe spacing to match your CNV detection needs. Strategically tiled probes in intergenic regions enhance sensitivity for even small CNVs.

        2. Seamless Workflow Integration

        Easily spike into your Twist Exome 2.0 panel and follow standard enrichment protocols—no workflow disruption, no extra training required.

        3. Evidence-Based Performance

        Validated with highly characterized samples, the panels consistently improve CNV calls, including those smaller than 50 kb, boosting confidence in your results.

        4. Flexible Panel Sizes

        Available in 2-reaction (16 samples) and 12-reaction (96 samples) formats to fit both small-scale research and high-throughput lab workflows.

        Download Poster on utilization of these panels with exome for cytogenetic research

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          DOWNLOAD POSTER >

          Why It Matters to You

          Reliable CNV detection isn’t just a technical metric—it directly impacts research outcomes, diagnostic accuracy, and patient care. Standard exome sequencing often misses CNVs due to uneven probe coverage, creating blind spots in your analysis. Twist CNV Backbone Spike-in Panels bridge those gaps, ensuring that even subtle copy number changes are identified, so you can make confident, data-driven decisions.

          For labs and clinicians, this means fewer follow-up tests, reduced time spent troubleshooting ambiguous results, and a smoother workflow. You can trust that your exome sequencing captures the variations that truly matter, whether for rare disease research, clinical diagnostics, or high-throughput screening.

          Moreover, the ability to choose between 25 kb, 50 kb, or 100 kb resolution gives you control over sensitivity and throughput, aligning with your project goals and patient population needs. Evidence-based validation demonstrates improved detection of CNVs—including those smaller than 50 kb—so your results aren’t just comprehensive, they’re actionable.

          By integrating these panels into your existing workflow, you enhance not only the quality of your data but also the efficiency of your lab operations, freeing time and resources for deeper analysis and patient-focused outcomes.

          Select the CNV Resolution You Need

          Select CNV Resolution You Need

          Table 1. Example data of Twist CNV Backbone Spike-in Panels. A highly characterized sample set known to contain CNVs (1) and a baseline set of 12 healthy individuals were sequenced with 2×150 reads on an Illumina NovaSeq 6000. The average number of SNVs, INDELs, and CNVs called and sequencing depth at each probe density was determined for each panel when spiked into Twist Exome 2.0 plus Comprehensive Spike-in. CNV calling was performed with a commercially available software solution (2)
           

          (1) Coriell Institute’s CNVPANEL01 – Human CNV Reference Panel.
          (2) eVai Platform (secondary workflow), enGenome Software. 

          Related Products

          Twist Exome 2.0

          Leading exome solution covering key genetic databases with high uniformity

          Twist Standard Hybridization Reagent Kit

          Reagents for high-efficiency NGS target enrichment (TE).

          Library Preparation Enzymatic Fragmentation Kit 2.0

          Enzymatic DNA fragmentation for efficient library prep.

          Ordering

          Higher Resolution: 

          110756  –  Twist 25kb CNV Backbone Spike-in Panel, 2 Reaction kit

          110757  –  Twist 25kb CNV Backbone Spike-in Panel, 12 Reaction kit

          Intermediate Resolution:

          110758 –  Twist 50kb CNV Backbone Spike-in Panel, 2 Reaction kit

          110759 –  Twist 50kb CNV Backbone Spike-in Panel, 12 Reaction kit

          Lower Resolution: 

          110760 –  Twist 100kb CNV Backbone Spike-in Panel, 2 Reaction kit

          110761 –  Twist 100kb CNV Backbone Spike-in Panel, 12 Reaction kit

          Exome Panels

          104132 –  Twist Exome 2.0, 2 Reactions, Kit

          104134 –  Twist Exome 2.0, 12 Reactions, Kit

          104136 –  Twist Exome 2.0, 96 Reactions, Kit

          105034 –  Twist Exome 2.0 plus Comprehensive Exome Spike-in, 2 Reactions

          105035 –  Twist Exome 2.0 plus Comprehensive Exome Spike-in, 12 Reactions

          105036 –  Twist Exome 2.0 plus Comprehensive Exome Spike-in, 96 Reactions

          *For research use only 

          Chris Wicky

          Clinical Genomics Manager - ANZ
          & Country Manager - NZ

          For guidance on integrating these panels into your operations, Decode Science can provide personalised support and local assistance.



          TALK TO ME DIRECTLY

          Twist Portfolio

          Twist NGS

          NGS

          Raise confidence in variant detection with superior target enrichment solutions

          Twist Oligo Pools

          Oligo Pools

          Precision, uniformity, and flexibility for results you can trust

          Synthetic Controls

          Synthetic RNA and DNA standards for assay development

          Twist Libraries

          Libraries

          Identify more hits and streamline screening with Twist's precise Variant Libraries

          Twist Genes

          Genes

          DNA Your Way: think bigger, expand your scope, and accelerate discovery in genes

          Twist Antibody

          Antibody

          Robust solutions for Antibody Discovery and Development by Twist Bioscience

          A CRE.AI.TIVE application of AI: Engineering a more resilient global food supply

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          A CRE.AI.TIVE application of AI: Engineering a more resilient global food supply

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            Phytoform Labs harnessed its AI-powered CRE.AI.TIVE platform to develop climate-resilient crops, with a focus on drought-tolerant tomatoes. By rapidly exploring millions of potential sequence edits, the platform identified 2,000 high-potential candidates for wet-lab validation.

            To overcome the challenge of synthesizing complex, AT-rich sequences with homopolymers, the team partnered with Twist Bioscience. Twist’s high-fidelity oligos ensured accurate transfer of AI-designed sequences to the lab, enabling efficient MPRA screening in tomato protoplasts.

            This AI-guided workflow validated predictions while streamlining experiments—reducing waste, saving resources, and ensuring only the most promising variants progressed to in vivo testing.

            Case Study Highlights

            1. AI-driven design of millions of sequence variants

            2. Efficient identification of high-impact edits, conserving time and resources

            3. Ensuring fidelity of AI-generated oligos for complex plant sequences

            4. Insights on impact and future directions



            DOWNLOAD FILE

            Chris Wicky

            Country Manager - NZ

            As the official distributor in Australia and New Zealand, Decode Science makes accessing genomics solutions straightforward. Our role is to connect your lab with advanced technologies, ensuring you get the right solution for your sequencing projects—delivered locally with support when you need it.



            Get In Touch Today

            Modified LongPlex™ Protocol (LongPlex XL)

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            Modified LongPlex™ Protocol (LongPlex XL)

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              This technical note outlines an alternative workflow for generating 10–15 kb HiFi reads from high-quality genomic DNA using the LongPlex Long Fragment Multiplexing Kit. In this approach, LongPlex is used to fragment and barcode samples, and PacBio’s Short Read Eliminator (SRE) is applied to size-select fragments >10 kb before SMRTbell® library preparation.

              LongPlex uses plate-based transposase tagmentation for multiplexed fragmentation and barcoding, removing the need for mechanical shearing and allowing barcoded samples to be pooled before SMRTbell prep. This simplifies the workflow, increases throughput, and lowers library prep costs.

              The standard LongPlex protocol generates 6–9 kb HiFi reads from high- to medium-quality DNA—ideal for microbial and other small-genome projects. However, users working with higher-quality DNA may want longer HiFi reads to maximize gigabase yield on PacBio systems.

              This modified workflow is only suitable for high-quality DNA (Femto Pulse GQN30kb ≥7). Using degraded DNA will result in substantial sample loss during SRE size selection.

              LongPlex™ XL Long Fragment Multiplexing



              View tech Note

              Chris Wicky

              Country Manager - NZ

              As the official distributor in Australia and New Zealand, Decode Science makes accessing genomics solutions straightforward. Our role is to connect your lab with advanced technologies, ensuring you get the right solution for your sequencing projects—delivered locally with support when you need it.



              Get In Touch Today

              Site Directed Mutagenesis

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              Mutants Synthesized. Mutagenesis Simplified.

              Make Site-Directed Mutagenesis precise, simple, and affordable with Twist’s Mutagenesis by Synthesis. Traditional site-directed mutagenesis can be time consuming and imprecise. Site directed mutagenesis through synthesis eliminates these complexities, providing you with precisely engineered sequences tailored to your exact specifications. From protein engineering to functional genomics, incorporate precision synthesis of mutants into your workflow and focus on achieving your research goals

              Why it matters to you:

              Precision engineering – 

              site-directed mutagenesis, custom DNA

              Flexible design –

              protein engineering, functional genomics

              Fast turnaround –

              Express Clonal Genes, rapid synthesis

              Cost-effective –

              affordable variants, scalable synthesis

              How to Qualify

              Product: Clonal Genes
              Number of mutation per genes: No Limit
              Mutation type: any number of point mutation,
              deletions, and/or insertions*
              Pricing: Discounts on every variant
              (Please kindly contact your Decode Sales Rep for enquiry)

              How it Works

              Terms and Conditions
              * This promo is only available to customers in the Asia Pacific region (excluding China and Japan). Pricing is subjected to changes.
              * To enjoy the special service of site directed mutagenesis, please quote your previous order number or approach Decode Science support.
              * Each mutation is defined as any combination of bases change within a 10 codon spread over the entire gene.
              * Average turnaround time for Express Clonal Genes is 4 -7 business days. This time will vary based on complexity and length of the sequence. Orders placed outside of the US will incur additional delivery turnaround time. Turnaround time for Clonal Genes is subject to change based on customizations and complexity. Additional specification: 50ng – 2 ug.



              Contact Decode Support >

              Watch Successful Implementations

              WEBINAR

              Stronger, tighter, and faster: designing new protein functions

              WEBINAR

              Designing Enzymes for the Infinite Recycling and Upcycling of Plastic

              WEBINAR

              Screening Gene Libraries to Rapidly Identify Immune-recognised Virulence Genes.

              Daina Elliott

              Business Development Manager

              As the authorized distributor of Twist Bioscience in Australia and New Zealand, Decode Science makes integrating Twist’s DNA synthesis solutions seamless. We connect your lab with high-throughput, high-fidelity Twist technology, enabling faster, more reliable genomic workflows—with local support and guidance every step of the way.



              Get In Touch Today

              Twist Portfolio

              Twist NGS

              NGS

              Raise confidence in variant detection with superior target enrichment solutions

              Twist Oligo Pools

              Oligo Pools

              Precision, uniformity, and flexibility for results you can trust

              Synthetic Controls

              Synthetic RNA and DNA standards for assay development

              Twist Libraries

              Libraries

              Identify more hits and streamline screening with Twist's precise Variant Libraries

              Twist Genes

              Genes

              DNA Your Way: think bigger, expand your scope, and accelerate discovery in genes

              Twist Antibody

              Antibody

              Robust solutions for Antibody Discovery and Development by Twist Bioscience

              Comparison of Evercode™ WT v3 and Chromium™ GEM-X Single Cell 3’ Kit v4 in Mouse Brain Nuclei

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              Comparison of Evercode™ WT v3 and Chromium™ GEM-X Single Cell 3’ Kit v4 in Mouse Brain Nuclei

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                Comparison Highlights

                1. Evercode WT v3 delivers superior gene detection in head-to-head sensitivity tests.
                2. Cell type proportions remain consistently represented.
                3. Analysis of differential gene expression shows Evercode WT v3 identifies 2× more genes than competing methods.

                WT-vs-Gem-x-mouse-brain-tech-note-experimental-design-1536x546

                Study Overview
                Two embryonic mouse brains were sagittally dissected and flash-frozen by a third-party vendor.
                One half from each brain was processed by a 10x Genomics certified provider for nuclei isolation and library preparation, while the other halves were processed by Parse Biosciences using their own workflow.
                Sequencing was performed by a third-party, and data analysis was completed using each manufacturer’s respective pipeline.



                View tech Note

                Dr. Ebru Boslem

                ANZ Market Manager - Research Genomics

                As the official distributor in Australia and New Zealand, Decode Science makes accessing genomics solutions straightforward. Our role is to connect your lab with advanced technologies, ensuring you get the right solution for your sequencing projects—delivered locally with support when you need it.



                Get In Touch Today

                Optimized CRISPR/Cas9 Gene Knockout PDF

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                Accelerate knockout experiments with XDel’s next-generation CRISPR design.

                EditCo Bio’s XDel technology eliminates the need for guide RNA pre-screening, using a coordinated multi-gRNA design that delivers consistently high on-target editing across immortalized, primary, and iPSC lines. Validated through 768 edited samples and 4,816 NGS libraries, XDel achieves robust knockout efficiency while minimizing off-target effects—saving time and improving reproducibility across diverse cell types.

                Validated performance. Proven precision.
                With a standardized amplicon sequencing QC workflow and high-throughput automation, XDel enables scalable, high-confidence Cas9-mediated editing for both pooled and single-cell clone analysis. Download the full guide to explore the data, workflows, and results behind EditCo Bio’s high-efficiency gene knockout strategy.

                Optimized CRISPR/Cas9 Gene Knockout pdf EditCo Bio

                Unlock to Download Sheet




                  Download Now >

                  Hamza Hassan

                  Business Development Manager

                  As the official distributor in Australia and New Zealand, Decode Science makes accessing genomics solutions straightforward. Our role is to connect your lab with advanced technologies, ensuring you get the right solution for your sequencing projects—delivered locally with support when you need it.



                  Get In Touch Today