Entries by Harshita Sharma

MGISP-NE32

PRODUCTS

MGISP-NE32

MGI NE32

MGISP-NE32 : Precision Extraction, Rapid Results

The MGISP-NE32 stands as a cutting-edge extractor, prioritizing both safety and efficiency. Distinguished by features such as an adjustable mixing module and UV pollution control, it establishes itself as a dependable and advanced option for nucleic acid extraction.

Efficient

Processing magnetic particles and extracting nucleic acid from 16/32 samples in 9 mins

Stable

Specialized heating block for 96-well plate ensures consistent heating

Safe

UV lamp and 96-well plate with disposable tip prevent sample cross-contamination

Flexible

Adjustable mixing module with varied modes; fits multiple magnetic particles and reagents

Automated Nucleic Acid Extraction Excellence

The MGISP-NE32 stands as a pioneering force in nucleic acid extraction, establishing unprecedented standards. Harnessing advanced magnetic rod technology, it efficiently handles a broad spectrum of samples. The platform’s distinctive adjustable mixing module, combined with the UV lamp and the 96-well pre-packaged plate, ensures unparalleled purity in extractions. Its intuitive interface streamlines program management, and adaptability with various particles and reagents enhances its versatility. The inclusion of a specially crafted heating block guarantees uniform results, solidifying the MGISP-NE32 as the ideal choice for professionals seeking speed, precision, safety, efficiency, versatility, intuitiveness, and consistency in nucleic acid extraction.

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    MGIFLP-L50

    PRODUCTS

    MGIFLP-L50

    MGIFLP-L50

    Developed independently by MGI, the MGIFLP-L50( stands as a modular sequencing workstation. This innovative platform is dedicated to establishing a novel sequencing model by seamlessly integrating the entire sequencing workflow into a single machine. The MGIFLP-L50 offers a fully automated solution, simplifying the process from sample to report with just one click.

    3 Labs →1 Lab

    Intelligent Experiment

    Disinfection System

    Pre-defined Workflow

    Features

    Consolidated Efficiency: MGIFLP-L50 optimizes laboratory space by minimizing the footprint of the instrument, consolidating the functionality of three traditional laboratory spaces into a modular sequencing workstation that occupies less than 2 m².

    Intelligent Experimentation:

    • Sample Barcode Scanning
    • Life-cycle Sample Tracing
    • Life-cycle Result Tracing
    • Intelligent Device Monitoring
    • Experimental Process Monitoring

    Streamlined Workflow: MGIFLP-L50 features a pre-defined workflow, setting a new standard for operational simplicity and making sequencing experiments more accessible.

    Advanced Disinfection System:

    • HEPA System: Achieving 99.995% efficiency at 0.3 μm
    • UV System: Providing irradiation higher than 100,000 uW.s/cm²

    MGI FLP50

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      MGISP-960

      PRODUCTS

      MGISP-960

      MGISP-960

      The MGISP-960 High-throughput Automated Sample Preparation System is a versatile, fully automated workstation featuring a 96-channel pipette. This system has validated numerous library kits, including WES/WGS/RNA, and boasts a fully automatic operation design. It enables unmanned processes and can be customized based on customer requirements. MGISP-960 stands as an efficient and widely-used automated sample preparation system.

      Economic & Efficient

      Autonomous Bulk Processing
      Minimal Manual Intervention

      Open Platform

      Tailored development crafted to meet specific and unique requirements.

      Comprehensive Protection

      UV Lamp & Flow Hood Comprehensive Contamination Prevention

      Comprehensive Functions

      Integrated PCR, Magnetic Rack & Temperature Modules

      Features

      Introducing the High-throughput Automated Sample Preparation Platform, MGISP-960, designed for swift plate-to-plate transfers in high-speed microplate processing applications such as library prep, ELISA assays, and screening. With its 96-channel pipetting tool, flexible deck configurations, and a standard microplate gripper, the platform accommodates a diverse range of applications. Its versatility extends to supporting various tip sizes, robotic microplate and cover movement, and integration with peripheral devices like a magnetic plate positioner and heater/shaker, enabling the efficient completion of full NGS methods. Reach out to us to explore how your methods align with our value-driven automation platforms.

      MGISP-960 boasts validated methodologies and library kits, covering:

      1. WGS
      2. Exome/Target Sequencing
      3. RNA/Transcriptome Sequencing
      4. Tumor mutation gene detection
      5. NIPT
      6. PGS/PGD
      7. PMseq
      8. Amplicon Sequencing

      MGI SP_960

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        MGISP-NEX

        PRODUCTS

        MGISP-NEX

        MGISP-NEX

        MGISP-NEX is a versatile automated nucleic acid extractor independently developed by MGI. It equips with an independent 8-channel pipetting module and magnetic rod extraction module. The independent 8-channel pipetting can perform liquid transferring, which reduces manual intervention and achieves a higher degree of automation.The magnetic rod extraction module has two types of magnetic rods: one with 24 magnetic rod heads and another with 96 magnetic rod heads, which can be flexibly interchanged.

        Versatile Liquid Handling:

        Our independent 8-channel pipetting system excels at liquid transfer, diminishing the need for manual intervention and ushering in a heightened level of automation.

        Advanced Disinfection System:

        Our triple anti-pollution design guarantees sample safety by significantly minimizing the risk of
        cross-contamination.

        Adaptable Extraction:

        Our innovative interchangeable magnetic rod module is designed for flexibility, catering to the diverse requirements of various scenarios.

        Features

        1. Barcode Scanner & Temp. Control Module:

          • Rapid scanning for tube barcode.
        2. Temp. Control Module
          • Cryogenic storage of reaction reagent.
        3. 8-channel Pipettor:

          • Independent control of pipetting distance, volume, and height.
        4. Rotatable Gripper:

          1. Real-time sensing of the grasping status.
        5. Anti-drip Baffle:

          • Prevention of cross-contamination within the experiment.
        6. MagneticRod Module:

          • Interchangeable 24 & 96 magnetic rod heads.
        7. UV System:

          • High-dose irradiation exceeding 100,000 µW.s/cm².
        8. HEPA System:

          • Efficient filtration to prevent contamination.

        MGI NEX

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          MGISP-NE384

          PRODUCTS

          MGISP-NE384

          MGISP-NE384

          MGISP-NE384: Precision Meets Speedy Automation

          The MGISP-NE384 is a state-of-the-art, high-throughput automated nucleic acid extractor designed for optimal purity and safety in extraction processes. Utilizing magnetic rod technology, it can efficiently extract and purify nucleic acid from 96/192/288/384 samples. The extractor features Z axis independent control and a robust HEPA filtration system, along with a UV lamp, ensuring a secure working environment for both nucleic acid extraction and lab technicians.

          Efficient

          Processing and mobilizing magnetic particles to extract and purify nucleic acid from 384 samples

          Stable

          Efficient 96-well plate reagent hardware design with disposable tip, reducing operational errors

          Safe

          HEPA filtration and UV lamp integration, eliminating cross-contamination and securing a safe workspace

          Flexible

          Independent Z-axis control in mixing & magnetic modules, with 1-4 module run options for 96/192/288/384 samples

          Features

          The MGISP-NE384 stands as a pinnacle in nucleic acid extraction, excelling in stability, intuitiveness, precision, flexibility, throughput, safety, and efficiency. This high-throughput automated platform utilizes sophisticated magnetic rod technology, allowing meticulous processing of 96/192/288/384 samples. Its unique mixing and magnetic module, featuring Z axis independent control, accommodates 1 to 4 modules for diverse sample sizes. The platform ensures a contamination-free environment with a HEPA filtration system and UV lamp, safeguarding the extraction process and lab technicians.

          The 23-inch built-in touch screen interface, powered by Windows software, provides an intuitive portal for easy program management. With a reliable hardware design, 96-well plate reagent, and disposable tips, the platform minimizes operational errors, delivering consistent and accurate results. For professionals seeking a blend of speed, flexibility, and safety in nucleic acid extraction, the MGISP-NE384 emerges as the top-tier choice.

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            MGISP-Smart 8

            PRODUCTS

            MGISP-Smart 8

            MGISP-Smart 8: Automated Pipetting Powerhouse

            The MGISP-Smart 8 features a self-contained 8-channel pipette, meticulously crafted to address a wide spectrum of pipetting requirements spanning from 1 μL to 1000 μL. This versatile system is compatible with microplates, reservoirs, and tubes, reducing the likelihood of human errors while enhancing efficiency with its integrated PCR machine, shaker, and temperature control capabilities. With its resilient adaptability, the MGISP-Smart 8 stands as an exemplary solution for precision and convenience in laboratory environments.

            Intelligent

            Intelligent Sensing & Barcode Scanning

            Configurable

            Slide Track Deck Design High Compatibility

            Viral Panels

            Integrated Pipetting Robot Arm Flexible and Efficient

            Features

            Introducing the MGISP-Smart 8 Precision Automated Pipetting System—a cutting-edge robotic solution designed for unparalleled efficiency and accuracy in your laboratory. Boasting an independent 8-channel setup, this automated pipetting robot seamlessly accommodates various lab instruments, including tubes, microplates, and reservoirs, guaranteeing a flawless pipetting experience.

            Equipped with a broad pipetting range from 1 μL to 1000 μL, the MGISP-Smart 8 goes beyond conventional limits. Its integration of advanced modules, such as the PCR machine and magnetic rack, presents a comprehensive solution to address diverse pipetting challenges in your lab.

            To further elevate your lab operations, the MGISP-Smart 8 comes equipped with sensing and barcode scanning functionalities, ensuring a streamlined and error-free process from start to finish. Experience precision and convenience at its finest with the MGISP-Smart 8 Precision Automated Pipetting System.

            MGI Smart 8

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              RNA Sequencing

              PRODUCTS

              RNA Sequencing

              RNA Sequencing

              Twist’s RNA sequencing workflows offer a complete NGS solution that produces uniform libraries for RNA sequencing.

              Twist offers targeted and whole transcriptome workflows that reduce time at the bench and integrate with our current set of NGS reagents, including our target enrichment kits. Each workflow delivers high quality libraries ready for sequencing from a wide range of inputs and sample types, including translation research samples.

              RNA Library Prep

              Twist offers two comprehensive RNA sequencing workflows: Targeted RNA Sequencing and Whole Transcriptome Sequencing. The Targeted RNA Sequencing workflow involves creating custom panels to focus on specific RNA transcripts, utilizing streamlined library preparation kits, unique dual indices, molecular identifiers, optimized target enrichment, and a proprietary exon-aware panel design algorithm. This allows for sensitive and efficient sequencing, even with low-quality RNA from FFPE samples.

               

              On the other hand, the Whole Transcriptome Sequencing workflow measures expression levels of various RNA species, including mRNA and lncRNA. It involves preparing libraries from total RNA extracted from both fresh and FFPE samples using Twist RNA Library Prep and Twist rRNA & Globin Depletion kit. This workflow enables RNA Library Preparation in less than 5 hours, providing a comprehensive view of the entire transcriptome. Two charts accompany the descriptions, detailing the targeted and whole transcriptome sequencing workflows.

              RNA Sequencing

              Twist Targeted RNA Sequencing Workflow

              Twist Whole Transcriptome Sequencing Workflow

              More Targets, Fewer Reads

              RNA Exome

              The collaboration of Twist RNA Exome, Twist RNA Library Prep, and Twist Target Enrichment forms a reliable toolkit for transcriptome sequencing, accommodating RNA from diverse sources, including FFPE samples. Notably, the RNA Exome enhances signal strength while requiring fewer sequencing reads, enabling the detection of low-expressing targets crucial for accurate transcriptional profiling. Its exon-aware design approach allows the identification of isoforms and junctions often overlooked in conventional methods, delivering precise and uniform sequencing reads tailored for analyzing protein-coding sequences within the human transcriptome.

               

              Designed exclusively for the human transcriptome, the RNA Exome targets 35.8 Mb bases, 19,708 genes, and 63,215 isoforms using sequences from Gencode and RefSeq. With over a 1.8-fold enrichment compared to whole transcriptome sequencing, it features a target enrichment approach built for RNA, incorporating an exon-aware probe design for protein coding regions, fusions, and isoforms. This design not only ensures precision but also reduces reads per sample, facilitating efficient analysis of multiple samples. The RNA Exome is versatile, compatible with FFPE samples and low RNA input. In practical tests, it demonstrates the capability to detect more targets with fewer reads across a range of RNA inputs, including FFPE and universal human reference RNA, using libraries prepared with the Twist RNA Library Prep.

              RNA Fusion

              The Twist Alliance CeGaT RNA Fusion Panel Kit emerges as a crucial tool in the precise detection of gene fusions, particularly impactful oncogenic driver mutations in various cancer types. Rapid and accurate detection holds significant clinical implications, guiding treatment decisions effectively. This RNA-based enrichment panel stands out by offering an enhanced targeted and sensitive approach, surpassing traditional methods.

               

              Curated in collaboration with CeGaT, a renowned genetic diagnostic and sequencing company in Europe, this panel encompasses 160 fusion genes associated with approximately 30 cancer types. Uniquely, it is designed not only for known gene fusions but also to unveil novel ones. The panel’s efficiency is amplified when used in conjunction with the Twist RNA Library Prep Kit, ensuring an end-to-end workflow with exceptional performance.

               

              With an optimized design featuring 7394 probes and a breakpoint design for 66 genes, the panel excels in covering a diverse array of cancer types. The incorporation of Twist core enrichment technology further maximizes capture efficiency, instilling confidence in RNA gene fusion detection. This panel is particularly well-suited for screening oncology samples, providing critical insights for treatment decisions or uncovering novel fusions for tumor classifications.

               

              *Note: The Twist Alliance CeGaT RNA Fusion Panel – 3 MB holds ISO-13485 certification, reinforcing its reliability in clinical applications.

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              NGS Portfolio

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                MRD Sequencing

                PRODUCTS

                MRD Sequencing

                MRD Sequencing

                Minimal Residual Disease (MRD), characterized by a small number of lingering malignant cells post-treatment, poses a recurrence risk often undetectable by standard surveillance methods. Twist Bioscience’s MRD Rapid 500 Panel addresses this challenge by utilizing circulating tumor DNA for early detection. Leveraging Twist’s silicon-based DNA synthesis platform, this scalable solution offers rapid turnaround time (as few as 6 business days) with personalized variant profiles from whole-genome or whole-exome sequencing. The MRD panels, featuring 50 to 500 probes, provide comprehensive genomic insights, compatible with Twist’s NGS library preparation and hybrid capture workflow. This facilitates early intervention and enhances personalized medicine approaches in cancer recurrence monitoring.

                Benefits

                Unmatched Scalability and Speed:
                Benefit from unparalleled scalability and efficiency as you capture variants of interest with panels ranging from 50 to 500 probes. Streamline your workflow by ordering up to 150 panels at a time, and experience the rapid shipment of panels within an impressive 6 business days.

                Customized Panel Designs:
                Tailor your panel designs to meet your specific needs using a variant target coordinate BED file. Improve capture performance by strategically filtering probe sequences over repetitive regions, leading to enhanced efficiency and significant cost savings on overall sequencing expenses.

                Panel Information:
                Leverage the advantages of a single plex for 12 tests, ensuring the detection of SNVs and small indels with high precision. Implement quality control through qPCR, and rest assured with ISO-13485 certification, signifying a commitment to reliability. Conveniently receive panels in Matrix Tubes, and benefit from designs supported against hg19 and hg38 reference genomes.

                Lab Workflow:
                Integrate seamlessly into your lab workflow with compatibility with Twist library preparation and hybridization workflows. Follow the recommended hybridization protocol, MRD Standard Hyb 2.0, ensuring not only efficiency but also a streamlined and harmonious laboratory process.

                MRD Rapid 500 Panel Design

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                  Long Read Sequencing

                  PRODUCTS

                  Long Read Sequencing

                  Long Read Sequencing

                  Combine the precision of Twist target enrichment with the power of long read sequencing to efficiently explore crucial genomic regions at scale. Twist Alliance panels, both pre-designed and customizable, empower researchers to capture target regions in a cost-effective and high-throughput manner, ensuring exceptional performance.

                  Exceptional Performance: Elevate your sequencing endeavors with panels specifically crafted for long read sequencing. Probes are optimized for high uniformity and sequencing efficiency, ensuring balanced coverage even across challenging-to-sequence or difficult-to-map genomic regions.

                  Long Read Sequencing at Scale: Efficiently scale up your sequencing projects with a protocol optimized for long fragment enrichment. This approach allows you to pack more samples into a single sequencing run, making it possible to study targeted regions across large cohorts.

                  Accurate Variant Calling with Targeted HiFi Sequencing from PacBio: Leverage the accuracy of targeted HiFi Sequencing from PacBio for precise variant calling of SNPs, SVs, and indels. Benefit from unambiguous haplotype resolution and long-range phasing, ensuring reliable results. This approach is compatible with Sequel IIe and Revio Systems, providing versatility in sequencing platforms.

                  Twist Alliance Dark Genes Panel
                  Enables sequencing of genes that are difficult or impossible to fully sequence with short read technology.

                  1. Targets 389 genes (20 Mb)
                  2. Key targets include GBA, SMN1/2

                  Twist Alliance Long-Read PGx Panel
                  Focus on important genes in pharmacogenomics that are critical to drug metabolism and patient therapeutic response.

                  1. Targets 49 genes (2 Mb)
                  2. Key targets include CYP2D6, HLA-A, HLA-B

                  Twist Alliance Dark Genes Panel

                  Twist Alliance Long-Read PGx Panel

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                    Library Preparation

                    PRODUCTS

                    Library Preparation

                    Library Preparation

                    Twist Library Preparation Kits offer a streamlined process for constructing high-quality DNA libraries in next-generation sequencing (NGS) applications. Tailored for whole genome sequencing and targeted enrichment, these kits simplify library preparation by combining multiple steps into a single reaction, enhancing efficiency and ensuring consistent results.

                    Two configurations are available to cater to different needs: Mechanical Fragmentation, designed for mechanically sheared gDNA, and Enzymatic Fragmentation, ideal for automated, high-throughput library preparation. Both configurations minimize artifacts, accommodate various DNA input types, and optimize sequencing of low-quality samples, with the Enzymatic Fragmentation offering tunable, reproducible fragment sizes while minimizing sequence bias and maximizing coverage depth.

                    Library Preparation Workflow

                    The Twist Library Preparation Kits provide a comprehensive solution for the entire library preparation workflow, encompassing crucial steps such as end repair, dA-tailing, adapter ligation, and library amplification. Both kits offer versatility in adapter choices, allowing the use of either full-length or universal adapters based on specific application requirements.

                    For the Enzymatic Fragmentation Kit, additional functionality includes the incorporation of enzymes for the fragmentation of genomic DNA (gDNA) samples. This feature enhances the kit’s capability for high-throughput library preparation.

                    The accompanying chart illustrates a robust library construction process by utilizing Twist Universal Adapters for insert ligation and UDI primer PCR amplification. This approach ensures a streamlined and efficient workflow, producing libraries suitable for a diverse range of applications.

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