Exome Panels
Twist Alliance Panels are a curated collection of high-quality, ready-to-use sequencing panels developed in partnership with leading scientific institutions. By combining Twist’s world-class oligonucleotide synthesis platform with our partners’ deep expertise, these panels provide comprehensive, validated coverage of clinically and scientifically relevant regions. Ready-made and scalable, they streamline workflows, save time and resources, and support research, clinical, and diagnostic applications—so your team can focus on discovery instead of design.
High-performance diagnostic exome panel for heritable diseases
When routine testing does not yield a diagnosis, DNA sequencing panels are often the next step—especially for suspected heritable conditions. Developed in collaboration between Victorian Clinical Genetics Services (VCGS) and Twist, the Twist Alliance VCGS Exome v2 provides comprehensive coverage of clinically relevant regions while maintaining high uniformity across the exome.
Key Features
Whole exome coverage (54.82 Mb) with enhanced capture of clinically significant genes (Mendeliome)
Dedicated coverage of pathogenic non-coding loci, ensuring clinically relevant variants outside traditional exons are captured
CNV detection backbone: probes across intergenic regions (~25 kb intervals) improve copy number variation calling
Highly uniform panel optimized for carrier screening, pre-natal, and post-natal testing
Probe Content
| Component | Number of Probes |
|---|---|
| Twist Exome 2.0 | 427K |
| VCGS-curated content | 101K |
| Total | 528K |
Ordering Options
111124 Twist Alliance VCGS Exome v2 – 54.82 Mb, 2 Reactions Kit
111126 Twist Alliance VCGS Exome v2 – 54.82 Mb, 12 Reactions Kit
111150 Twist Alliance VCGS Exome v2 – 54.82 Mb, 96 Reactions Kit
Flexible exome panel for research and clinical discovery
Designed in collaboration with the Broad Institute Genomics Platform, the Twist Alliance Clinical Research Exome uses validated clinical patient data to provide comprehensive exome coverage. This panel enables efficient investigation of cancer, rare, and inherited disease-associated regions while maintaining high uniformity and throughput.
Key Features
Comprehensive exome coverage (34.9 Mb) with flexibility for content customization using Twist’s NGS platform
Supplemental enrichment of clinically relevant coding and non-coding regions, including cancer-associated loci and rare disease genes
High uniformity probes allow cost-efficient per-sample processing and high throughput—successfully applied to >250,000 samples at the Broad Institute
Validated design for research and clinical discovery
Panel Content Highlights
| Component | Description |
|---|---|
| Twist Core Exome | Standard exome coverage |
| Mitochondrial Genome | Full coverage of mitochondrial DNA |
| ACMG73 Genes | Clinically actionable targets |
| OMIM & COSMIC Regions | Supplemental coverage of disease- and cancer-associated loci |
| Broad-defined Targets | Additional validated coding and non-coding regions |
Ordering Options
104032 Twist Alliance Clinical Research Exome – 34.9 MB, 2 Reactions Kit
104033 Twist Alliance Clinical Research Exome – 34.9 MB, 12 Reactions Kit
104034 Twist Alliance Clinical Research Exome – 34.9 MB, 96 Reactions Kit
Twist Exome 2.0 is designed to detect rare and inherited diseases, as well as germline cancers. This panel’s high uniformity and low off-target rate deliver best-in-class sequencing efficiency, enabling quality data to be collected with less sequencing. With superior coverage of major genetic databases (RefSeq, CCDS, GenCode, Clinvar, ACMG73 and more) and the addition of clinically relevant non-coding pathogenic and likely pathogenic variants, Twist Exome 2.0 provides the value of multiple clinical panels all wrapped into one, easily customizable package.
Chris Wicky
Clinical Genomics Manager - ANZ & Country Manager - NZ
Get a complete overview of Twist Alliance Panels, including panel coverage, workflow efficiency, and application scope. For guidance on selecting or integrating these panels into your research or diagnostic operations, Decode Science provides personalized support and local assistance.
The mouse is an extremely important model system for studying genetic variation, tumor mutations, and phenotypic outcomes as well as the therapeutic effect of pharmaceutical agents. As genetic variant databases are continuously updated, the Twist Mouse Exome panel is thoughtfully designed and built from the most current databases. When combined with Twist’s expanding portfolio of library preparation and enrichment reagents, the complete toolset allows researchers to achieve industry-leading coverage across target regions while optimizing sequencing cost and sample throughput.
Understanding the genetic variations among dog breeds is crucial for unraveling the genetic regulation of traits and understanding the basis and progression of diseases in dogs. Comprehensive gene panels, such as exomes, play a pivotal role in enhancing veterinary diagnostics and associated clinical medicine. These Next-Generation Sequencing (NGS) panels, like the Twist Alliance Canine Exome developed in collaboration with the Broad Institute’s Karlsson lab, are essential tools for advancing canine genomic research. They contribute to improved understanding of canine cancers and potential therapeutics.
The Twist Alliance Canine Exome panel is designed to achieve the following objectives:
- Covers coding exons of canine genes.
- Facilitates comparative genomic studies between canine and human genomes.
- Includes regions of known importance in human cancers.
- Allows for cost-effective deep sequencing.
Furthermore, canine genomic research has demonstrated benefits for human medical research, revealing genetic similarities between human and canine tumors, such as Copy Number Variations (CNVs), differential gene expressions, and structural chromosome abnormalities. This interdisciplinary approach underscores the potential for insights gained in canine genetics to contribute to advancements in human medicine.
Twist Library Prep Enzymatic Fragmentation 2.0 Kit
Enzymatic DNA fragmentation for efficient library prep. Learn More >
Twist Fast Hybridization and Wash Kit
Speeds up hybridization and wash steps in NGS target enrichment (TE). Learn More >
Twist Universal Adapter System
Flexible adapters for seamless 'T-A' overhang ligation library preparation. Learn More >
