Library Preparation

January 23, 2024/in NGS, Partners, Twist/by Harshita Sharma

PRODUCTS

Library Preparation

Twist Library Preparation Kits offer a streamlined process for constructing high-quality DNA libraries in next-generation sequencing (NGS) applications. Tailored for whole genome sequencing and targeted enrichment, these kits simplify library preparation by combining multiple steps into a single reaction, enhancing efficiency and ensuring consistent results.

Two configurations are available to cater to different needs: Mechanical Fragmentation, designed for mechanically sheared gDNA, and Enzymatic Fragmentation, ideal for automated, high-throughput library preparation. Both configurations minimize artifacts, accommodate various DNA input types, and optimize sequencing of low-quality samples, with the Enzymatic Fragmentation offering tunable, reproducible fragment sizes while minimizing sequence bias and maximizing coverage depth.

Library Preparation Workflow

The Twist Library Preparation Kits provide a comprehensive solution for the entire library preparation workflow, encompassing crucial steps such as end repair, dA-tailing, adapter ligation, and library amplification. Both kits offer versatility in adapter choices, allowing the use of either full-length or universal adapters based on specific application requirements.

For the Enzymatic Fragmentation Kit, additional functionality includes the incorporation of enzymes for the fragmentation of genomic DNA (gDNA) samples. This feature enhances the kit’s capability for high-throughput library preparation.

The accompanying chart illustrates a robust library construction process by utilizing Twist Universal Adapters for insert ligation and UDI primer PCR amplification. This approach ensures a streamlined and efficient workflow, producing libraries suitable for a diverse range of applications.

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NGS Portfolio

Custom Panels

Viral Panels

Methylation Detection

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purePlex™ DNA Library Prep Kit

January 17, 2024/in Low-pass Whole Genome Sequencing, Microbial Sequencing, Partners, SeqWell/by Harshita Sharma

PRODUCTS

purePlex™ DNA Library Prep Kit

A benefit to purePlex is that, because of its simplicity, users can quickly and seamlessly incorporate the kit with existing methods for high-throughput pipetting.

Speed, Performance, and Auto-Normalization with Unique Dual Indexes

2.5-hour workflow for 96 samples, 45 min. hands-on time
Auto-normalization of read counts and insert size over 10-fold input range
Unique dual indices included

Key Features

Fast, flexible workflow with no requirement for full plate processing
Auto-normalization reduces QC burden, improves data consistency
Early pooling for easier sample handling
Reduced GC bias compared to other transposase-based methods
Significant cost and plastics savings

purePlex™ DNA Library Prep Kit Workflow seqWell

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SeqWell Portfolio

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ExpressPlex™ Library Prep Kit

January 17, 2024/in Partners, Plasmid and Amplicon Sequencing, SeqWell/by Harshita Sharma

PRODUCTS

ExpressPlex™ Library Prep Kit

Designed for quick turnaround of plasmid, amplicon, or synthetic construct sequencing, ExpressPlex* is the fastest high-throughput library preparation kit available (based on total time to prepare 96 – 384 samples).

90-min workflow with 30-min or less hands-on
Fragmentation, barcoding, and amplification in 1 step
No primers/barcodes to buy
Virtually cross-talk free
Up to 6,144 samples prepped and sequenced in 24 hours
NEW: 384-well ultra-high throughput version available

*Patents Pending

Benefits of using ExpressPlex

ExpressPlex allows you to spend your time on data and results, not pipetting. There are solutions for low-, medium-, and high-throughput labs. For ultra-high throughput users, we now offer 384-well versions that will enable you to multiplex up to 6,144 samples in a single run.

Go from extracted samples to libraries on the sequencer in < 1/2 a day
Sequence more samples for less
Easily automate your protocol
Train any lab tech, minimize chance for error
Choice of 96 or 384-well versions to fit your workflow
Reduce labor while increasing efficiency
Decrease chance for errors via minimal handling steps
Everything included: no complex supply chain management of barcodes and primers

ExpressPlex 96-Well Workflow

ExpressPlex 384-Well Workflow

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SeqWell Portfolio

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Tagify

January 17, 2024/in Gene Editing QC, Partners, SeqWell/by Harshita Sharma

Are You Confident in Your Gene Editing Results?

If you’re not validating every edit with high-quality sequence analysis, you’re guessing — and guessing has no place in CRISPR, TALEN, or ZFN workflows.

Accurate gene editing QC is essential for verifying edits, detecting off-target events, and protecting downstream research or therapeutic development. Robust sequencing isn’t optional — it’s your safety net.

Scalable, High-Fidelity QC for Every Gene Editing Workflow

On-Target & Off-Target Gene Editing QC

Tagify® Adapter-Loaded Transposase for High-Confidence Tagmentation

Accurate characterization of on-target and off-target editing events is non-negotiable. Insertions, deletions, inversions, translocations — every outcome needs to be detected and verified. Yet standardized QC methods for gene editing are still early-stage, especially for off-target analysis. Most teams end up navigating inconsistent protocols, variable reagent quality, and limited scalability.

Reliable, QC-Verified Reagents for Tagmentation-Based Gene Editing Assays

Tagify reagents remove the uncertainty. Each lot is fully QC-checked and delivered as ready-to-use or custom-loaded hyperactive Tn5 or TnX, seqWell’s next-generation transposase engineered for dependable performance.

With Tagify, you get:

Consistent tagmentation performance
Scalable workflows for high-throughput QC
Reagents optimized for sensitive off-target detection

Broad Compatibility with Leading Gene Editing QC Methods

Tagify reagents integrate seamlessly with widely used transposase-based assays including:

GUIDE-seq²
UDiTaS
CHANGE-seq
RGEN
TTIS-seq
and additional emerging QC workflows

*Some transposase-based methods require appropriate licensing.

Useful Webinars

If you’re looking to integrate these QC solutions into your pipeline, Decode Science can provide hands-on guidance and local expertise.

Happy Customer Feedback

"We’ve been asking for this. What’s great about Tagify is that it allows you to look at a specific place in the gene, and adapter concentration is taken care of. This system is really important because it provides us this opportunity to assess the consequences of gene editing in a semi-unbiased way. It shortens our process, makes it much more controlled, and lessens the amount of reagents we need to use.”

– Athea Vichas, Ph.D., Senior Principal Scientist of Gene Editing Analytical Development, Bristol Myers Squibb

“ExpressPlex is literally faster than Sanger. This changes everything for us. Basically, taking a two-day process to one day dramatically shortens time to data.”

– Henry Chan, Ph.D., Synthetic Biology Lead, Octant Bio

SeqWell Portfolio

plexWell LP 384

January 17, 2024/in Low-pass Whole Genome Sequencing, Microbial Sequencing, Partners, SeqWell/by Harshita Sharma

PRODUCTS

plexWell LP 384

The plexWell™ LP 384 kit is engineered for low-pass whole genome library preparation and sequencing, utilizing the enhanced plexWell workflow. The kit includes major reagents essential for library preparation, featuring Magwise™ paramagnetic beads (DNA polymerase not included). This kit allows efficient library preparation for more than one 96-well plate of samples and normalizes input DNA over a wide range of 5-25 ng.

plexWell™ LP 384 (Low-Pass Whole Genome) Highlights:

NGS multiplexed library generation kit designed for Illumina® platforms.
Features assay-ready 96-well fully-skirted low-profile PCR plates in sets of 4 (4 x 96).
Provides 2,304 (96 x 24) unique barcode combinations.
Includes 6 sets of 4 pool barcodes (PB Set A, B, C, D, E, or F).

Recommended Application: Ideal for low-depth whole genome/GBS (Genotyping by Sequencing) coupled with imputation and analysis software for comprehensive genomic studies.

plexWell™ Library Prep Workflow:

plexWell™ Library Prep Chemistry:

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SeqWell Portfolio

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plexWell

January 17, 2024/in Microbial Sequencing, Partners, Plasmid and Amplicon Sequencing, SeqWell/by Harshita Sharma

PRODUCTS

plexWell

plexWell 96

The plexWell™ 96 (PW096) kit streamlines NGS multiplexed library generation for Illumina® platforms, housed in an assay-ready 96-well fully-skirted low-profile PCR plate. The kit includes major reagents, featuring Magwise™ paramagnetic beads (DNA polymerase not included), facilitating efficient library preparation for one 96-well plate inputs. Notably, it offers normalization of input DNA over a broad range of 3-30 ng, with a cost-saving volume-based pricing structure that can reduce total lab costs by 30-50%.

Key Features:

Comprehensive kit with Magwise™ beads.
Efficient library prep for one 96-well plate.
Normalization of input DNA (3-30 ng).
Volume-based pricing for cost savings.

Recommended Applications: Ideal for large-scale full-length viral surveillance, Synthetic Construct Sequencing (Amplicons, Plasmids, BACs, etc.), microbiome screening, microbial whole-genome sequencing, scRNA-seq (single-cell RNA sequencing), and low-depth whole-genome/GBS (genotyping by sequencing).

plexWell 384

The plexWell™ 384 (PW384) kit is a comprehensive solution for NGS multiplexed library generation on Illumina® platforms. Each kit includes major reagents, featuring Magwise™ paramagnetic beads (DNA polymerase not included), essential for efficient library preparation for more than one 96-well plate of samples. This kit offers normalization of input DNA across a wide range of 3-30 ng, with a cost-saving volume-based pricing structure, reducing total lab costs by 30-50%.

Key Features:

Comprehensive kit with Magwise™ paramagnetic beads.
Efficient library prep for more than one 96-well plate.
Normalization of input DNA (3-30 ng).
Cost-saving volume-based pricing.

Recommended Applications:

Large-scale full-length viral surveillance.
Synthetic Construct Sequencing (Amplicons, Plasmids, BACs, etc.).
Microbiome screening.
Microbial whole-genome sequencing.
scRNA-seq (single-cell RNA sequencing).
Low-depth whole-genome/GBS (genotyping by sequencing).

plexWell™ Library Prep Workflow:

plexWell™ Library Prep Chemistry:

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SeqWell Portfolio

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Clonal Genes

January 16, 2024/in Partners, Synbio Genes, Twist/by Harshita Sharma

PRODUCTS

Clonal Genes

Overview

Twist Bioscience’s advanced platform is designed to meet diverse DNA synthesis needs, with the capability to synthesize hundreds of thousands of genes each month. Utilizing a silicon-based platform for DNA synthesis, Twist delivers highly precise, sequence-perfect clonal genes of various lengths and complexities, all verified through Next-Generation Sequencing (NGS). Now, with the introduction of the Express Genes service, Twist offers even faster turnaround times, synthesising NGS-verified Clonal Genes in as few as 5 days.

Twist Bioscience’s Express Genes service offers researchers a cost-effective, customizable, and scalable solution for obtaining high-quality clonal genes with efficient turnaround times. This service empowers researchers to accelerate their projects and advance their scientific endeavors with confidence.

Low Cost – High Quality:

Priced from 18¢ (AUD) per base pair.

No hidden sub-cloning or DNA complexity fees.

Delivery in as fast as 12 business days.

DNA Your Way:

Customize 0.3 – 5 kb genes cloned into a plasmid of your choice.

Choose from Twist Catalog Vectors or provide your own.

Four preparation scales (50ng – 2µg | 2µg – 10µg | 10µg – 100µg | 100µg – 1mg).

Options for normalization and endotoxin-free available.

Scalable Synthesis:

No order limits, providing flexibility.

Same turnaround time regardless of order size.

Express Genes Service

Introducing Twist Bioscience’s Express Genes service*— synthesising NGS-verified, sequence-perfect Clonal Genes with an unprecedented order-to-ship turnaround time of 5-7 business days. Explore our full Clonal Genes offering below and experience the speed and efficiency of Express Genes for your research needs.

*Terms and Conditions: Eligible Express Genes ship in 5-7 business days. This time will vary based on complexity and length of the sequence. Orders placed outside of the US will incur additional delivery turnaround time. Turnaround time for Clonal Genes is subject to change based on customizations and complexity. Average turnaround time for Clonal Genes is 10-15 business days. New vector onboarding for both Express Genes and Clonal Genes will add additional time.

DATA

The data presented illustrates the high quality and precision of Twist Bioscience’s Clonal Genes, showcasing a graphical representation of the standard Next-Generation Sequencing (NGS) verification performed on each clonal gene. The featured clonal gene in the figure serves as an example of an error-free clone. The read depth is indicated for the entire plasmid, and no Single Nucleotide Polymorphisms (SNPs) or insertions/deletions (indels) were detected, emphasizing the accuracy and reliability of Twist’s clonal gene synthesis.

This data emphasizes Twist’s commitment to providing researchers with sequence-perfect clonal genes, ensuring high-quality and error-free results for a wide range of applications in the field of genetic research and synthetic biology.

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Synbio Genes Portfolio

Gene Fragments

Clonal Genes

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Gene Fragments

January 16, 2024/in Partners, Synbio Genes, Twist/by Harshita Sharma

PRODUCTS

Gene Fragments

Overview

Twist Bioscience offers Synthetic Gene Fragments as a fast, economical, and efficient solution for building genes in your research. These gene fragments enhance the cloning process by minimizing the need for colony screening, saving valuable time and reducing overall costs associated with cloning and sequencing.

By leveraging Twist’s Synthetic Gene Fragments, researchers can think bigger, design on a grander scale, and accelerate their discoveries. With the advantage of an industry-leading error rate, customizable lengths and yields, and the convenience of no order limits, Twist’s Gene Fragments offer a cost-effective and time-efficient solution for diverse research needs. To learn more or place an order, researchers can explore the product sheet or directly proceed with ordering from Twist Bioscience.

Fast and Economical:

Gene Fragments for assembly and cloning.
Compatible with all downstream cloning methods.
Priced from 13¢ (AUD) per base pair.
Synthesised in as little as 2 business days.

Screen Less, Discover More:

Industry-leading error rate of 1:7500.
Length: 300 bp – 1800 bp.
Yield: 100 ng – 1 µg.
No order limit.

Twist Bioscience’s DNA synthesis technology stands out by outperforming competitors with exceptionally low error rates. In a direct comparison of Gene Fragment products, Twist consistently demonstrated the lowest error rate, as illustrated in the graph comparing Twist and Integrated DNA Technologies, Inc. (IDT). The results reveal that Twist Gene Fragments exhibit greater sequence accuracy compared to eBlocks and gBlocks, boasting an average of 2-fold greater accuracy in sequence fidelity over gBlocks.

Moreover, Twist Gene Fragments consistently yield the highest percentage of perfect clones, offering a significant advantage in terms of time and cost savings for researchers. The graph showcasing a direct comparison of the percentage of sequence perfect clones across various gene lengths and sequences for three different gene products highlights Twist’s superiority. The data, derived from a set of 63 sequences with diverse gene lengths, reflects the broad applicability of Twist’s technology in addressing the varied requirements of real-world synthetic biology applications.

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SynBio Genes Portfolio

Gene Fragments

Clonal Genes

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High Throughput Antibody Production

January 16, 2024/in Partners, Synbio Antibody, Twist/by Harshita Sharma

PRODUCTS

High Throughput Antibody Production

Twist Bioscience introduces High-Throughput Antibody Production, a revolutionary gene-to-protein workflow designed to address bottlenecks in antibody production. This innovative process leverages Twist’s silicon-based DNA synthesis platform, capable of precisely writing thousands of genes in a single run. The result is rapid and high-throughput production of tens to thousands of diverse antibodies, facilitating efficient screening for biophysical and pharmacokinetic properties.

As a bonus, Twist offers downstream characterization and screening services for all high-throughput antibody production orders. This comprehensive service includes affinity ranking, epitope binning, and more, providing researchers with a streamlined solution for antibody discovery.

The key benefits of Twist’s High-Throughput Antibody Production include the creation of sequence-perfect antibodies, all generated in-house using NGS-verified clonal genes. The workflow offers unlimited capacity, enabling simultaneous production at a scale ranging from tens to thousands of antibodies. Additionally, it is fully customizable, allowing users to select custom antibody sequences, vectors, and isotypes according to their specific research needs.

Researchers can seamlessly expand their antibody production pipeline by uploading the desired antibody sequences, allowing Twist to handle the rest of the process. To learn more about ordering antibodies and taking advantage of this cutting-edge technology, visit the ordering tab for detailed information. Twist Bioscience’s High-Throughput Antibody Production offers a game-changing solution for researchers seeking rapid and customizable antibody discovery and screening.

HOW IT WORKS

The High-Throughput Antibody Production process at Twist Bioscience involves several key steps:

Synthesis and Cloning: Twist synthesizes and clones heavy, light, or single-chain variable sequences into Fc-containing vectors.
Transfection: Paired heavy and light chain or single-chain genes are transfected into HEK293 cells for a period of 4 days.
Harvesting: Supernatants containing the secreted antibodies are harvested.
Purification: Antibodies are purified using Protein A/G.
Inspection: Purified antibodies undergo inspection for concentration, size, and purity using A280 and digital CE-SDS.

Regarding the timeline, from sequence submission to delivery, the process takes approximately 20-25 business days for 1 mL orders and 22-35 business days for 8 mL orders. This efficient and streamlined workflow allows researchers to receive their customized antibodies in a relatively short timeframe, making it a valuable solution for high-throughput antibody production needs.

DATA

Twist Bioscience offers a robust and accelerated antibody screening process, as illustrated in the provided figure. Following antibody production, a total of 518 antibodies were synthesized and purified. After rigorous quality control tests, an impressive 482 antibodies passed the size and purity assessments, with 409 antibodies yielding more than 100 μg per antibody.

The robust yield is determined using LabChip for every purified sample. This involves denaturing the sample to obtain both size and purity data. Capillary electrophoresis sodium dodecyl sulfate (CE-SDS) is then run to collect sizing data benchmarked to a ladder. This provides approximate molecular weight (kDa) and a percent purity for both heavy and light chains. The peak at 7 kD represents the dye front in the analysis.

For those interested in additional data on aspects such as affinity and titer levels, Twist Bioscience offers further insights in their downloadable flyer. This comprehensive screening process demonstrates Twist’s commitment to delivering high-quality antibodies with robust yields, making it an efficient and reliable solution for accelerated antibody production and screening needs.

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Synbio Libraries Portfolio

Site Saturation Variant Library

Combinatorial Variant Library

Spread Out Low Diversity Library

CAR
Library

TCR
Library

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TCR Library

January 16, 2024/in Partners, Synbio Libraries, Twist/by Harshita Sharma

TCR Libraries

Adoptive cell therapy (ACT) has transformed treatment options for patients with advanced or refractory cancers by redirecting the immune system to recognize and eliminate malignant cells.

Engineered TCR therapies represent a major branch of ACT, using synthetic or optimized T-cell receptors to target tumor-specific antigens. The workflow typically begins with sequencing the tumor biopsy to identify actionable mutations and the peripheral blood to map the patient’s TCR repertoire.

TCR repertoire profiling can be performed using bulk or single-cell sequencing, each offering distinct advantages:

Bulk sequencing provides broad coverage of the repertoire and captures large numbers of clonotypes, but does not preserve α–β chain pairing.
Single-cell sequencing preserves α–β receptor pairing and full receptor architecture, but at a much lower throughput than bulk methods.

But.... Why Twist for TCR Library Construction?

Twist’s combinatorial TCR library platform gives you the power to systematically reshape and expand your TCR landscape. Instead of being limited to naturally occurring α–β pairs, you can mix and match chains to uncover novel receptor combinations with therapeutic potential.

This approach pairs especially well with bulk repertoire sequencing, where α–β pairing is not preserved. Combinatorial assembly fills that gap by generating all possible pairings across defined alpha and beta chains.

Example:
A library built from 4 α-chains and 4 β-chains yields 16 unique α–β combinations — complete coverage, not a partial subset.

And The Key Benefits Are ...

Download Product Sheet, Flyer on T-Cell Receptor Discovery & Advanced DNA Tools

Unlock with quick sign up!

Performance Data You Can Rely On

TCR discovery isn’t limited by ideas — it’s limited by how fast you can screen and rebuild candidates. The real bottleneck comes from needing to evaluate large numbers of alpha–beta pairings to find the rare binders that actually matter.

Combinatorial TCR Libraries break that bottleneck.

By shuffling alpha and beta chains across all possible pairings, these libraries recreate a dramatically broader search space than what exists in the native repertoire. Instead of being restricted to the pairings captured in your sequencing data, you can now generate thousands of rationally designed combinations in a single build.

This enables teams to:

Scale screening throughput by producing many more testable TCR variants
Expand functional diversity beyond naturally occurring pairings
Capture rare or non-intuitive binders that would never appear in arrayed or bulk sequencing formats
Shorten discovery timelines by moving directly from variant design → library synthesis → high-throughput screening

For support implementing combinatorial TCR libraries into your discovery workflow, Twist Bioscience and Decode Science can provide hands-on guidance and localized expertise.

T Cell Receptor (TCR) Discovery workflow

Partner with Twist Bioscience to accelerate the identification and development of advanced cell therapies using large-scale, highly diverse T-cell receptor (TCR) libraries.

Twist provides highly uniform, NGS-verified libraries composed of precise, user-defined combinations of alpha and beta gene fragments. These libraries enable:

Comprehensive Screening: Efficiently test all desired TCR combinations to uncover high-affinity, functional receptors.
Customizable Design: Define specific alpha-beta pairings to target particular antigens or therapeutic applications.
High Uniformity: Libraries are optimized for reproducibility and coverage, ensuring consistent performance across screens.

This approach streamlines TCR discovery, empowering researchers to explore broader sequence diversity and accelerate the development of next-generation engineered TCR therapies.