PRODUCTS
EditCo’s Gene Knockout Kit offers a simplified, highly efficient solution for generating human and mouse protein-coding gene knockouts. Unlike traditional CRISPR methods, which rely on a single guide RNA (gRNA) to create random indels, EditCo’s multi-guide approach ensures consistent and precise gene disruption. This method generates fragment deletions at the targeted loci, providing a more reliable and predictable knockout, ultimately enhancing experimental success. With fast delivery times (kits arrive in just 5 days), users can avoid the trial and error of guide screening and proceed quickly with their experiments. The process is streamlined further with an easy online ordering portal, which includes the option to purchase controls, SpCas9 nuclease, and Transfection Optimization kits to complement your knockout experiment. This solution guarantees high knockout efficiency, making gene editing faster and more reliable than ever before.
Guaranteed for human and mouse genes (excluding non-essential genes), ensuring high knockout efficiency.
Chemically modified sgRNA resists degradation and prevents immune responses for reliable gene editing.
Optional add-ons like the Transfection Optimization Kit, SpCas9 nuclease, and controls for a comprehensive experiment.
Includes multiguide sgRNA, SpCas9 EZ Scaffold, buffers, water, and quality control documents for PCR and sequencing.
EditCo’s Gene Knockout Kit uses a multi-guide gRNA approach to ensure reliable and efficient gene disruption. This method targets a single exon with three spatially coordinated gRNAs, creating multiple double-strand breaks and resulting in large fragment deletions. Compared to single-guide CRISPR, our multi-guide strategy significantly increases knockout efficiency, with over 75% large deletions and a 29.2% improvement in knockout success.
Image: Better knockout efficiency was found across 32 genetic targets assessed.
Image: EditCo’s Gene Knockout Kit was designed to increase the efficacy of CRISPR-mediated knockouts.
Image: Multi-guide gRNA for each target resulted in >75% large deletion outcomes.
Image: Editing of three genes (CDK9, CINP, COASY) in HEK293 cells (transfected via nucleofection) using the multi-guide approach resulted in high knockout efficiencies, as indicated by Knockout (KO) Scores
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