High-uniformity, low-error-rate oligo pools synthesised on silicon. Up to 300nt, unlimited pool size, industry-leading representation for CRISPR screening, tiling libraries, FISH probes, and more.
Up to 300nt
No pool size limit
Error rate up to 1:3000
From 2- 4 days synthesis
Shipping ~ 1 week.
Upgrade your Oligo Pools to Cloned Oligo Pools and let Twist Bioscience handle the amplification and cloning. Get your custom library ready for downstream screening while saving 50% on cloning fees.
PROMO CODE:
Offer valid through 31 December 2026
*50% off cloning fees. Valid for ANZ customers through 31 Dec 2026. Final pricing subject to project scope — request a custom quote. Cannot be combined with other discounts. Quote promo code ANZEOY2026 at order submission. Contact Decode Science to place an order today!
50%
off cloning fees*
Highly diverse single-stranded oligonucleotides synthesised on Twist’s silicon-based platform. Massively parallel production of hundreds of thousands of accurate oligos per run — bias-free, with complete sequence representation and industry-leading uniformity. Ideal for CRISPR guide libraries, tiling screens, MPRAs, and FISH probes.
DELIVERY TIME: from 2- 4 days synthesis
Twist synthesises, amplifies, and clones your pool library directly into a vector — removing the complexity of PCR optimisation, polymerase selection, and cloning workflow design. Chimera rates as low as 1.5%, high uniformity, and low error rates across pools up to 300nt including high-GC content sequences.
DELIVERY TIME: from 6 – 8 weeks synthesis & cloning
Unmatched uniformity — no representation bias
Over 90% of oligos within <2.0x of the mean. More unique targets per screen, less sequencing effort required to achieve coverage depth — fewer wasted experiments chasing underrepresented targets.
Oligos up to 300nt — encode more per design
Multiple guides, homology arms, minimal promoters, and regulatory elements in a single oligo. Extended architecture removes the need for design compromises on complex applications.
Industry-leading error rate of up to 1:3000
Critical for MPRAs and applications where a single base substitution is the biological signal. Independent researchers report twofold fewer mutations than expected versus competing providers.
Cloned pools — chimera rates as low as 1.5%
Twist’s in-house amplification eliminates hybrid molecules formed by suboptimal PCR conditions. Consistent performance across pools up to 300nt, including high-GC content sequences.
Designing a CRISPR library, tiling screen, or FISH probe panel? Our team can advise on pool design and format before you order.
Twist Oligo Pools represent collections of single-stranded oligonucleotides synthesized using state-of-the-art silicon-based DNA writing technology. The innovative synthesis platform employed by Twist Bioscience allows for the simultaneous production of large quantities of high-quality and accurate oligos in each run. This capability facilitates the creation of complex and diverse oligo pools, specifically advantageous for applications such as CRISPR screening.
The process involves users submitting their oligo sequences to Twist Bioscience, which then synthesizes user-designed oligo pool libraries. This service enables researchers to dedicate their time to experimental work and discovery, with the synthesis handled by Twist.
The quality of Twist oligos is underscored by highly uniform synthesis, as evidenced by Next-Generation Sequencing (NGS) quality control data. A representative oligo pool containing 23,000 90mer oligos demonstrates remarkable uniformity, with the corresponding table indicating the metrics for this pool. Twist oligos are synthesized with minimal bias, ensuring high uniformity and complete representation of oligos in the pool.
Furthermore, sequencing analysis of Oligo Pools generated by Twist Bioscience is compared with those from an array-based competitor. The results highlight the superiority of Twist Oligo Pools, as they consistently contain 100% of the expected sequences and exhibit a higher percentage of correct sequences when compared to the competitor’s pool. This emphasis on accuracy and completeness in sequence representation makes Twist Oligo Pools a robust and reliable choice for various molecular biology applications.
New Cloned Oligo Pools from Twist Bioscience represent a cutting-edge solution aimed at ensuring the foundational quality of oligo pools for successful experiments. Recognizing that errors during synthesis or cloning can adversely impact oligo pool quality by causing over and underrepresentation of desired sequences, Twist now provides an optimized cloning service. This service streamlines the experimental process by alleviating the need for researchers to navigate challenges such as testing PCR amplification conditions, selecting appropriate polymerases and primer pairs, and designing a cloning workflow.
How does it work?
The process involves just two steps. Users submit their oligo sequences to Twist Bioscience, and the company takes care of synthesizing, amplifying, and cloning the user-designed oligo pool libraries. This approach allows researchers to dedicate their time to experimentation and discovery while outsourcing the intricacies of oligo pool synthesis and cloning to Twist Bioscience.
Twist Bioscience emphasizes the high quality of its cloned oligo pools across all lengths, showcasing performance data for pools under 150 nucleotides and those up to 300 nucleotides in length, even with high GC content. The data highlight the uniformity achieved in both cases.
CRISPR screens, tiling library assays, MPRAs, and FISH probe panels all share the same dependency: the quality of the data coming out is capped by the quality of the oligo pool going in. Underrepresented sequences create blind spots. High error rates generate false positives. Chimeric sequences in cloned pools corrupt the intended library composition. Each of these problems is addressable at the synthesis step — but only if the synthesis method is capable of meeting the standard required.
Twist Oligo Pools and Cloned Oligo Pools are most relevant for:
Guide RNA libraries for genome-wide knockout, interference, or activation screens where uniformity directly determines hit sensitivity and false positive rate. Longer oligos allow multiple guides, homology arms, and promoter elements to be co-encoded in a single design.
Comprehensive tiling of regulatory regions, promoters, and enhancers requires dense, uniform representation across complex sequences including palindromic and repetitive elements that other synthesis methods handle poorly. Single base substitution accuracy is non-negotiable for functional assays.
Rapid development of tunable, multiplexed FISH probes for spatial visualisation of nucleic acids. Sequence control and oligo lengths up to 300nt enable highly specific probes across large numbers of genomic targets.
Screening large sequence spaces for protein function, stability, or interaction requires pools that faithfully represent the designed diversity — not a biased subset of it. Twist’s uniformity ensures the screen reflects your design intent.
Business Development Manager
| Specification | Oligo Pools | Cloned Oligo Pools |
|---|---|---|
| Oligo length | Up to 300nt | Up to 300nt |
| Pool size | No limit | No limit |
| Yield | >0.2 fmol average per oligo | 50 µg plasmid DNA (up to 250 µg + glycerol stocks available) |
| Uniformity | >90% within <2.0x of the mean | >90% within <5.0x of the mean |
| Error rate | Up to 1:3000 | Low — validated across lengths and GC content |
| Chimera rate | N/A | As low as 1.5% |
| Turnaround | 2–4 business days | 4–6 weeks (<150nt) / 6–8 weeks (<300nt) |
| ANZ pricing | Contact Decode Science | Contact Decode Science |
Turnaround times reflect facility processing before handover to carrier. Delivery times vary by location. New vector onboarding adds
Full-length gene synthesis up to 7kb — consistent per-base pricing
Bespoke target enrichment — pairs with tiling oligo pool design workflows
High-fidelity library prep validated end-to-end with Twist reagents
Oligo Pools are delivered as single-stranded oligonucleotides ready for your own amplification and cloning workflow. Cloned Oligo Pools go further — Twist synthesises, amplifies, and clones the pool for you, delivering plasmid DNA at 50 µg yield. If you want to skip PCR optimisation, polymerase selection, and cloning design, Cloned Oligo Pools remove that burden entirely.
No. Twist’s silicon-based synthesis platform places no practical limit on unique oligos per pool — whether your experiment requires tens of thousands or over a million unique sequences, pool size is not a constraint.
For Oligo Pools, over 90% of oligos are represented within less than 2.0x of the mean. For Cloned Oligo Pools, uniformity is within 5.0x of the mean, reflecting the additional amplification and cloning steps involved.
Up to 1:3000 — one of the lowest available at this scale and length. Independent researchers running MPRAs have reported twofold fewer mutations than expected using Twist pools compared to competing providers.
Chimeras are hybrid molecules formed when suboptimal PCR conditions cause recombination between different oligos during amplification. They introduce off-target sequences that compromise library composition. Twist’s in-house amplification achieves chimera rates as low as 1.5% — significantly lower than standard PCR-based approaches.
Oligo Pools ship in 2–4 business days from order confirmation. Cloned Oligo Pools take 4–6 weeks for pools up to 150nt and 6–8 weeks for pools up to 300nt. New vector onboarding adds an additional 2 weeks.
Yes. Both product formats are validated to maintain high uniformity and low error rates across high-GC content pools at both lengths up to 150nt and 300nt.
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