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Don’t waste your sequencing reads on abundant and uninformative sequences, which limits the detection of lower abundance signals.
Jumpcode Genomics’ Deplete X technology removes fragments prior to sequencing by harnessing CRISPR-Cas9 to degrade uninformative sequences in next generation sequencing(NGS) libraries.
This can boost detection of low-level gene expression, somatic mutation detection and better pathogen detection.
Abundant ribosomal, mitochondrial, insulin and housekeeping genes
Ribosomal, mitochondrial, and non-variable genes
Repetitive DNA, contaminating human DNA in microbial samples
DepleteX operates through a process of mediated depletion, seamlessly integrated into Next-Generation Sequencing (NGS) library preparation protocols at strategic points tailored to specific applications. The method involves the formation of CRISPR-Cas9 complexes, utilizing a pool of meticulously designed guide RNAs.
These CRISPR-Cas9 complexes are directed to target sequences of interest within the genomic DNA. Upon binding, Cas9 initiates precise cuts at the targeted sequences, rendering them inaccessible for PCR amplification. Consequently, these targeted sequences are effectively excluded from the resulting sequencing library.
By leveraging DepleteX, researchers can precisely remove undesired sequences from their NGS libraries, allowing for focused analysis of specific genomic regions or elements of interest while minimizing background noise and maximizing sequencing efficiency.
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