In genomics, consistency and scalability are everything. Whether you’re running a small validation assay or a full-scale discovery project, the last thing you need is variable probe quality or supply constraints slowing you down. That’s where Twist Bioscience’s silicon-based DNA synthesis platform changes the game.
Each silicon chip can produce over one million unique single-stranded DNA oligos in a single run, with every oligo synthesized on an individually addressable surface. This level of precision means every sequence you order is exactly what your experiment needs—no wasted pools, no uneven representation.
Because of the platform’s massive synthesis capacity, there’s virtually no limit to the number of unique oligos that can be combined into a single pool. It’s DNA manufacturing at a scale that matches your research ambitions—whether you’re designing CRISPR libraries, NGS target panels, or high-throughput screening assays.
Twist Oligo Pools are built to remove the typical bottlenecks in genomics workflows: inconsistent yields, batch variability, and long lead times. With a scalable, silicon-based process, researchers get reproducible, high-fidelity oligos that perform reliably across projects—so experiments move forward faster and cleaner.
Oligos up to 300 nt let you encode more in every design.
Exceptional pool uniformity delivers more unique targets per screen, reducing effort, time, and cost.
Precise, uniform synthesis with low error rates—ensuring complete, reliable data across any pool size.
Business Development Manager
As the official distributor for Twist Bioscience in Australia and New Zealand, Decode Science provides easy access to Twist Oligo Pools—high-quality, scalable DNA synthesis solutions for genomics research. By connecting local laboratories with Twist’s advanced silicon-based synthesis platform, Decode Science ensures researchers can design, order, and receive the oligo pools they need with confidence, precision, and reliable local support.
You share your oligo sequences. Twist handles the rest.
Using its advanced silicon-based DNA synthesis platform, Twist Bioscience produces highly uniform, bias-free oligo pool libraries so you can focus on running experiments, not troubleshooting variability.
Each oligo pool is built with precise sequence control and verified using next-generation sequencing (NGS)–based quality checks. In a typical test of 23,000 90-mer oligos at 300x read coverage, Twist consistently demonstrates exceptional uniformity and balanced representation across all sequences.
Independent sequencing analysis shows Twist Oligo Pools deliver 100% of expected sequences, outperforming traditional array-based synthesis methods that often show gaps or inconsistencies. The result is a reliable, reproducible DNA library you can trust from the first run — with data quality that holds up at any scale.
| Oligo Length | Up to 300nt |
| Oligo Pool Size | No limits on pool size |
| Yield | >0.2 fmol average of each oligo |
| Uniformity | >90% oligos represented within <2.0x of the mean |
| Error rate | Up to 1:3000 |
| Turn-Around Time | 2-4 business days* |
| Price | Industry-leading pricing |
The number of unique oligos per pool depends on your experimental needs. Twist’s silicon-based synthesis platform supports pools ranging from hundreds to over a million sequences without sacrificing uniformity.
Yes. Twist Oligo Pools can be customized for highly specific targets, including CRISPR libraries, mutagenesis studies, hybrid capture, or any sequence requiring precise coverage.
Twist uses NGS-based quality control to ensure even representation and uniform synthesis of all sequences, minimizing bias and dropout in downstream applications.
Absolutely. Pools are synthesized to be fully compatible with automated workflows and sequencing pipelines, making them scalable from small experiments to genome-wide screens.
Each pool undergoes sequencing analysis to confirm that all intended sequences are present and correctly synthesized, ensuring reliable and reproducible experimental results.
Reach out to the Decode Science team—we’re ready to provide instant guidance and support.
As the official Twist Bioscience distributor in Australia and New Zealand, Decode Science connects your lab with Twist’s advanced synthesis platform, making it easy to design, order, and receive high-quality oligo pools with reliable local support.
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