QuantumSeq delivers direct, drop-in replacements for BigDye® and POP-7™ — validated for identical performance, with no protocol changes required.
QuantumSeq is a complete suite of Sanger sequencing reagents engineered as direct substitutes for the industry-standard BigDye® Terminator chemistry. The QuantumDye™ Cycle Sequencing Kits are supplied as 2.5x concentrated ready-reaction premixes, available in v1.1 and v3.1 formulations to match your existing instrument settings and DyeSet calibrations exactly — no revalidation, no workflow disruption. Whether your lab runs de novo sequencing, resequencing, heterozygote detection, or routine mutation screening, QuantumSeq kits slot directly into your current SOP using the same dilution ratios, thermal cycling conditions, and capillary electrophoresis parameters.
For labs dealing with GT-rich or G-rich templates that cause early signal dropout, the QuantumDye™ Terminator dGTP v3.1 Kit substitutes dITP with dGTP to extend reliably through difficult secondary structure regions. Pair any QuantumDye kit with QuantumSAP™ PCR Product Cleanup Reagent — a dual-enzyme ExoSAP-IT™ replacement — to eliminate residual primers and unincorporated dNTPs before sequencing, and with QuantumPOP™ 7 polymer for CE separation on Applied Biosystems 3730/3730xl DNA Analyzers. Every component in the QuantumSeq range is manufactured in the USA and available through Decode Science with local support.
The v1.1 formulation is a direct drop-in for BigDye® Terminator v1.1, using DyeSet E with no instrument recalibration required. It delivers uniform peak heights and optimised signal balance specifically tuned for applications that demand strong basecalling adjacent to the primer — including short PCR product templates run on rapid electrophoresis modules and heterozygote or mixed-base detection workflows. Available in 24-, 100-, and 1,000-reaction formats (QSD1-024, QSD1-100, QSD1-1000).
The v3.1 kit is the standard workhorse replacement for BigDye® Terminator v3.1, using DyeSet Z. It produces long, high-confidence reads with extremely low background across de novo sequencing and resequencing applications — results that are indistinguishable from the BigDye® reference in head-to-head comparisons. Suitable for the broadest range of template types and the most commonly used Sanger sequencing workflows. Available in 24-, 100-, and 1,000-reaction formats (QSD3-024, QSD3-100, QSD3-1000).
Where standard dITP-based terminator chemistry produces early signal dropout in GT-rich or G-rich sequence contexts, the dGTP v3.1 kit extends confidently through those regions by substituting dGTP for dITP — without requiring any change to your DyeSet Z calibration or run module. It is a direct replacement for dGTP BigDye® Terminator v3.0 and is particularly valuable for repetitive elements, GC-rich amplicons, and secondary-structure-prone templates. Available as QSDG3-100 (100 reactions).
QuantumPOP™ 7 is a direct substitute for POP-7™ polymer for capillary electrophoresis on Applied Biosystems 3730 and 3730xl DNA Analyzers. No changes to instrument settings, workflow, or calibration are required — simply allow the refrigerated polymer to equilibrate to ambient temperature before loading and proceed as normal. Available in four pack sizes to suit both low-throughput labs and high-volume core facilities: QSP7-28 (28 mL), QSP7-140 (5 × 28 mL), QSP7-280 (10 × 28 mL), and QSP7-840 (30 × 28 mL).
QuantumSAP™ is a cost-effective drop-in replacement for ExoSAP-IT™, combining Exonuclease I and recombinant Shrimp Alkaline Phosphatase (rSAP) to remove residual single-stranded primers and unincorporated dNTPs directly in your completed PCR reaction — no buffer exchange required. A simple two-step protocol (37 °C for 10 min, then 80 °C for 5 min) prepares your PCR product for sequencing or genotyping. Available in a 50-reaction evaluation size (QSSP-S50), 500-reaction (QSSP-500), and 2,000-reaction (QSSP-2000) formats.
BigDye® Terminator reagents have long been the de facto standard for Sanger sequencing, but procurement costs have remained a persistent pressure point for core facilities and research labs running high reaction volumes. QuantumSeq was designed specifically to resolve this without requiring labs to compromise on data quality or invest in workflow revalidation.
For a core sequencing facility running hundreds of reactions per week, reagent cost is one of the most controllable operating variables — but only if the substitute performs identically and integrates without friction. QuantumSeq kits meet both criteria: they are validated for direct substitution at the same dilution ratios (1:8 to 1:16), use the same DyeSet calibrations (DyeSet E for v1.1 chemistry, DyeSet Z for v3.1 chemistry), and produce data that is indistinguishable from the BigDye® reference in head-to-head comparisons. For labs that regularly encounter difficult GT-rich or G-rich templates, the dGTP v3.1 variant adds specialist capability without adding complexity. And because every kit ships with pGEM® Control DNA and M13(-21) Control Primer, sequencing run QC remains consistent with your existing controls strategy.
HOW YOU ACHIEVE THE RESULTS
Getting Started: Your Existing SOP is Already Compatible
Switching to QuantumSeq requires no revalidation of your instrument, calibration files, or run modules. The transition follows three straightforward steps:
Select the corresponding QuantumDye™ kit: v1.1 (QSD1 series, DyeSet E) for near-primer and mutation detection applications; v3.1 (QSD3 series, DyeSet Z) for standard long-read workflows; or dGTP v3.1 (QSDG3 series, DyeSet Z) for GT-rich and G-rich templates. If you currently use POP-7™ polymer and/or ExoSAP-IT™, select QuantumPOP™ 7 and QuantumSAP™ as direct replacements.
Use 0.5–1.0 µl of QuantumDye Cycle Sequencing Mix per reaction (1:16 to 1:8 dilution), exactly as specified in your current SOP. No changes to cycle number, extension temperature, or purification protocol are required. For PCR cleanup, add 2.0 µl of QuantumSAP™ reagent directly to your completed PCR reaction, incubate at 37 °C for 10 minutes, then inactivate at 80 °C for 5 minutes — the same two-step protocol as ExoSAP-IT™.
All QuantumSeq product inserts are available for download via the link below. To request pricing or confirm SKU availability for your reaction volume, submit an enquiry through the form below and a Decode Science specialist will respond within one business day.
No. QuantumDye v1.1 kits use DyeSet E and QuantumDye v3.1 (including the dGTP variant) use DyeSet Z — the same DyeSet assignments as BigDye® Terminator v1.1 and v3.1 respectively. No instrument recalibration is required.
The recommended working range is 0.5–1.0 µl of the 2.5x Cycle Sequencing Mix per reaction, corresponding to a 1:16 to 1:8 dilution. This is identical to the dilution range used with BigDye® Terminator and can be used without modification to your existing protocol.
v1.1 chemistry is optimised for applications requiring strong signal close to the primer, short PCR product templates with rapid electrophoresis run modules, and more accurate base assignments in heterozygous or mixed-base positions. v3.1 is the general-purpose formulation for standard long-read Sanger sequencing. If you are currently using BigDye® v1.1, use QuantumDye v1.1; if you are using BigDye® v3.1, use QuantumDye v3.1.
The QuantumDye™ Terminator dGTP v3.1 Kit (QSDG3-100) was specifically developed for GT-rich, G-rich, and other difficult-to-sequence templates. It replaces dITP with dGTP to extend reliably through regions that cause premature signal loss with standard dITP-based chemistry.
Yes. Exonuclease I and rSAP in QuantumSAP™ are active in commonly used PCR buffers, so no buffer exchange is needed. Simply add 2.0 µl of QuantumSAP™ reagent directly to your completed PCR reaction.
Yes. QuantumPOP™ 7 is a direct substitute for POP-7™ polymer on ABI 3730 and 3730xl DNA Analyzers with no changes to user protocol, workflow, or instrument settings. Allow the refrigerated polymer to equilibrate to ambient temperature before loading.
QuantumSAP™ is available in a 50-reaction sample size (QSSP-S50) for evaluation. QuantumDye kits are available in 24-, 100-, and 1,000-reaction formats. QuantumPOP™ 7 is available in 28 mL, 5 × 28 mL, 10 × 28 mL, and 30 × 28 mL formats. Contact Decode Science to discuss evaluation options for your lab.
Yes. All QuantumSeq products are for research use only and are not intended for use in diagnostic procedures.
We only need these information to serve you better. Decode Science respects your privacy and will never spam you with unrelated content.
Simply add your details below and get quick access to the brochure.
Simply add your details below and get quick access to the brochure.
Simply add your details below and get quick access to the brochure.
