The Twist TrueAmp Polymerase Mix is an engineered amplification reagent purpose-built for NGS library workflows — not adapted from qPCR or cloning applications, but designed from the ground up for the demands of complex library amplification. At its core is a proofreading, high-processivity DNA polymerase formulated to deliver uniform coverage across the full GC spectrum, from AT-rich to GC-rich targets, while minimising the amplification-induced errors and homopolymer slippage that compromise variant calling accuracy. Whether you’re amplifying pre-capture or post-capture libraries, TrueAmp Polymerase Mix keeps bias low and your data trustworthy.
Delivered as a single-tube 2× mastermix with aptamer-enabled hot start, TrueAmp Polymerase Mix is stable at room temperature and ready for automated liquid handling — reducing setup complexity, minimising handling errors, and supporting same-day or overnight hybridisation workflows alongside Twist target enrichment kits. It is validated end-to-end with Twist library preparation and enrichment reagents, making it a natural fit for laboratories already running Twist workflows who want consistent amplification performance without introducing an unvalidated variable. As your authorised Australian distributor, Decode Science can advise on integration into your current library prep and enrichment protocols.
TrueAmp Polymerase Mix sustains normalised coverage across the full GC content range — including AT-rich organisms such as C. difficile and GC-rich organisms such as B. pertussis — where other polymerases show progressive coverage dropout. This performance is maintained as PCR cycle number increases, making it reliable for both low-cycle pre-capture amplification and higher-cycle post-capture workflows.
Figure 1. GC-normalized coverage; AT-rich C. difficile and GC-rich B. pertussis.
Improved amplification efficiency means TrueAmp reaches yield targets in fewer PCR cycles than standard NGS polymerases. Fewer cycles directly reduces the accumulation of duplicate reads and cycle-induced artefacts — preserving library complexity and sequencing efficiency, particularly from low-yield post-capture libraries where every cycle counts.
Figure 2. Serial dilutions from 1 ng to 100 fg, Qubit and TapeStation QC.
Engineered proofreading activity measurably reduces C→T misincorporations introduced by cytosine deamination — one of the most common sources of false positive variant calls in NGS data, and particularly prevalent in FFPE-derived libraries. Improved homopolymer fidelity further reduces a known source of indel artefacts in repeat-containing regions, supporting cleaner variant calls across the full spectrum of library types.
Figure 3. Substitution rates after >7M base incorporations; homopolymer readout using Twist clonal plasmids.
Magnetic beads used for library purification and size selection can carry over into PCR reactions and inhibit amplification, reducing yields and introducing variability. TrueAmp Polymerase Mix is formulated to maintain robust performance in the presence of magnetic bead carryover — removing a common source of batch-to-batch inconsistency in post-purification amplification steps.
Figure 4. Tolerance to Paramagnetic Beads During PCR. PCR reactions were spiked with 6.25 μl, 12.5 μl, 25 μl, and 50 μl of MyOne T1 Beads, M270 Beads (Invitrogen), and Twist DNA Purification Beads. Reactions were purified post bead-removal and quantified on Qubit dsDNA Broad Range Assay.
When sequencing results are inconsistent or coverage is uneven, the first instinct is usually to look at sample quality, capture chemistry, or bioinformatics. Amplification polymerase is rarely the first variable examined — but for libraries with challenging GC content or low input, it is often where the problem originates.
TrueAmp Polymerase Mix is most relevant in these contexts:
GC-extreme regions are routinely underrepresented in enriched libraries. TrueAmp’s uniform coverage across 5–95% GC content ensures those targets are captured and sequenced with the same confidence as GC-neutral regions.
After hybridisation capture, the library is often at its most precious and most limited in quantity. An amplification reagent that achieves yield targets in fewer cycles with less bias preserves the complexity and integrity of that material.
Reduced C→T misincorporation and homopolymer slippage directly improves the signal-to-noise ratio for low-frequency variant detection — critical for tumour heterogeneity studies, liquid biopsy, and minimal residual disease monitoring.
Deamination damage in FFPE DNA generates artefactual C→T changes that a standard polymerase will faithfully amplify. TrueAmp’s engineered proofreading reduces this signal, preserving the accuracy of variant calls from archival material.
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TrueAmp Polymerase Mix is an engineered proofreading, high-processivity DNA polymerase formulated specifically for NGS library amplification. Unlike standard PCR enzymes optimised for single-amplicon applications, TrueAmp is designed to amplify complex NGS libraries with uniform GC coverage, high fidelity, and consistent yields — minimising the bias and artefacts that standard enzymes introduce when applied to library amplification.
TrueAmp Polymerase Mix generates consistent, high-yield libraries down to 100 pg input or below. Contact Decode Science for guidance on cycle number optimisation at very low inputs.
TrueAmp Polymerase Mix is validated end-to-end with Twist library preparation and enrichment reagents. It is compatible with pre-capture and post-capture amplification steps and with same-day and overnight hybridisation workflows alongside Twist target enrichment kits. Contact Decode Science to confirm compatibility with your specific protocol configuration.
TrueAmp Polymerase Mix has been validated with Twist library prep and enrichment reagents. For use with other library prep systems, contact Decode Science to discuss compatibility and any protocol adjustments that may be required.
No. The aptamer-enabled hot start provides room temperature stability for setup without compromising PCR performance. It eliminates the need for ice or cold block handling during reaction assembly, simplifying manual workflows and supporting automated liquid handling integration.
Yes. TrueAmp’s engineered proofreading activity reduces C→T misincorporations caused by cytosine deamination — a common artefact in FFPE-derived DNA — making it particularly well-suited to post-capture amplification from FFPE libraries where deamination damage is elevated.
TrueAmp Polymerase Mix produces libraries compatible with Illumina sequencing platforms. Contact Decode Science for platform-specific guidance.
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