EditCo’s Gene Knockout Kit offers a simplified, highly efficient solution for generating human and mouse protein-coding gene knockouts.
Unlike traditional CRISPR methods, which rely on a single guide RNA (gRNA) to create random indels, EditCo’s multi-guide approach ensures consistent and precise gene disruption. This method generates fragment deletions at the targeted loci, providing a more reliable and predictable knockout, ultimately enhancing experimental success.
With fast delivery times (kits arrive in just 5 days), users can avoid the trial and error of guide screening and proceed quickly with their experiments. The process is streamlined further with an easy online ordering portal, which includes the option to purchase controls, SpCas9 nuclease, and Transfection Optimization kits to complement your knockout experiment.
This solution guarantees high knockout efficiency, making gene editing faster and more reliable than ever before.
XDel’s multi-guide CRISPR design consistently delivers higher on-target editing across multiple loci and cell types. By coordinating 2–3 sgRNAs per exon, researchers can achieve complete functional knockouts with minimal variability.
Off-target edits are a major concern in gene editing. XDel’s cooperative guide approach allows for lower gRNA concentrations while maintaining efficiency, reducing unintended edits and improving experimental reliability.
Edited cell pools maintain stable protein loss for weeks, enabling direct use in downstream functional assays. This reduces validation cycles and accelerates your research timelines.
Despite its power, CRISPR-Cas9 isn’t foolproof. Most conventional knockout strategies rely on a single guide RNA to induce a small insertion or deletion (indel) at the target site. In theory, this should disrupt the reading frame and deactivate the gene — but in practice, it rarely works that cleanly.
Even minor indels can restore partial functionality or trigger alternative splicing, leaving residual protein activity that undermines downstream results. What should be a “complete knockout” often ends up a mosaic of mixed edits, forcing researchers to spend additional time validating, recloning, or repeating experiments.
And it doesn’t stop there — when working with complex or sensitive cell models like iPSCs, low editing efficiency and variable expression recovery make reproducibility a constant battle. The result? Weeks lost, inconsistent phenotypes, and data that’s difficult to interpret or publish.
Researchers need more than chance-based gene disruption.
They need an approach that targets with precision, edits consistently across cell types, and produces true functional knockouts the first time.
That’s exactly where XDel Technology steps in.
XDel Technology – Coordinated, Multi-Guide Editing
Traditional CRISPR depends on a single cut — a gamble on where repair enzymes will introduce small, random indels. XDel takes a fundamentally smarter approach.
Instead of one guide, XDel uses up to three sgRNAs targeting sites within the same exon. These guides work together to generate coordinated fragment deletions, ensuring the targeted region is fully removed rather than partially disrupted. The result is a true functional knockout — not a mixture of partial edits.
This multi-guide design provides several key advantages:
Higher knockout efficiency: Coordinated cuts increase the likelihood of complete loss of gene function across all alleles.
Cleaner edit outcomes: Large, predictable deletions are easier to confirm with standard PCR or sequencing, reducing downstream validation time.
Compatibility across cell types: Proven performance in immortalized, primary, and iPSC models — even those typically resistant to editing.
By shifting from random indels to engineered deletions, XDel dramatically improves experimental reproducibility and shortens the path from edit design to verified knockout.
Business Development Manager- Research and Applied
XDel isn’t theory — it’s backed by real, quantitative data across multiple loci and cell types.
In comparative studies of seven endogenous genes across four different cell lines, XDel’s multi-guide approach consistently outperformed conventional single-guide designs in both knockout efficiency and edit consistency.
Traditional single-guide methods introduce random indels that often yield partial or incomplete knockouts.
XDel solves this by deploying up to three predesigned sgRNAs targeting a single exon, working together to generate large fragment deletions.
Across seven endogenous loci in four cell lines, this approach achieved significantly higher and more consistent editing efficiencies — reducing the rework often required in CRISPR experiments.
Effective gene disruption means sustained loss of protein function, not just transient edits.
In K562 cells, XDel-mediated knockouts of four membrane proteins maintained stable protein depletion for up to 21 days post-editing — with >90% cell viability throughout.
These pools are immediately assay-ready, eliminating the need for additional validation or re-editing.
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A key concern with multi-guide systems is off-target activity — but XDel’s design delivers the opposite.
Rigorous NGS analysis across 63 off-target loci confirmed that XDel edits are more precise than single-guide approaches, showing markedly lower off-target frequencies across both immortalized and iPSC lines.
Western blot analyses for five independent target genes confirmed complete and consistent protein depletion at days 7, 14, and 21 post-transfection.
This demonstrates that XDel doesn’t just produce edits — it reliably eliminates functional protein expression across diverse gene targets.
The cooperative behavior of XDel’s multi-guide system enables lower total gRNA usage while maintaining exceptional on-target editing rates.
Even when RNP concentrations were reduced to 0.25×, editing remained high with minimal off-target impact — translating to more cost-efficient and cleaner edits.
Decode Science is the official distributor of EditCo in Australia and New Zealand, helping researchers access XDel Knockout Technology with local expertise and technical support. Our team works alongside you to identify the right solutions for your workflow — from targeted knockouts to large-scale CRISPR screens — backed by EditCo’s proven data and Decode’s commitment to reproducibility.
For more information or project-specific guidance, reach out to Hamza Hassan, our business development manager, to discuss how XDel can accelerate your next experiment.
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