Atrandi Biosciences has built something that changes the fundamental constraint of single-cell analysis. Every trade-off in conventional single-cell workflows — the inability to add or exchange reagents mid-experiment, the limits on running sequential biochemical steps on the same cell, the gap between what you want to measure and what you can actually measure — traces back to the same problem: the compartment. Standard droplets are closed systems. Atrandi’s Semi-Permeable Capsules (SPCs) are not. By creating a compartment that retains cells and nucleic acids while freely exchanging small molecules such as enzymes, buffers, and primers, SPCs enable multi-step, multi-omic workflows on the same individual cell — with bulk-like chemistry, at single-cell resolution, and without compromising either.
Atrandi offers two platform paths depending on your workflow requirements. Flux is an accessible, push-button instrument for researchers who want to run Atrandi’s validated application kits — including single-cell microbial genomics and DNA + RNA co-sequencing — without developing their own microfluidics workflow. Onyx is a versatile research platform for groups developing next-generation single-cell assays, offering full control over droplet and SPC generation, reagent addition, and active droplet manipulation. As Atrandi’s authorised Australian and New Zealand distributor, Decode Science can advise on platform selection, application fit, and getting started with either system.
Unlike standard droplets, which are closed once formed, SPCs are semi-permeable — they retain cells and high-molecular-weight nucleic acids while allowing small molecules to pass freely in and out. Multi-step workflows.
By running sequential DNA and RNA capture workflows on the same encapsulated cell, Atrandi enables direct, simultaneous profiling of both modalities from a single cell, connecting genotype and phenotype without averaging.
Individual microbial cells at throughput that makes population-scale single-cell sequencing practical, enabling characterisation of microbial dark matter, strain-level diversity, and rare variants that bulk approaches systematically miss.
Flux delivers SPC encapsulation at the press of a button — up to 100,000 cells across 8 samples simultaneously in 30 minutes, paired with Atrandi’s validated reagent kits for immediate application deployment.
Semi-Permeable Capsules have an aqueous core and sieve-like walls that keep cells and nucleic acids partitioned inside — while allowing reagents, buffers, and media to flow freely in and out.
This means you can run sequential reactions on the same cells, in a single tube, with a standard pipette. Add a reagent. Wash it away. Add the next one. Every step runs under its own optimal conditions, on the same individual cells, without ever losing them between steps.
No closed droplets. No compatibility constraints. No specialist microfluidics skills required.
SPCs are generated at the press of a button — via Flux for validated application workflows, or Onyx for flexible research and assay development.
Perform reactions on single-cells with standard lab equipment
Run every molecular biology reaction under optimal conditions
Analyze thousands-to-millions of single-cells in parallel in a single tube
Compatible with diverse cell types and biochemical reactions
Unlimited nutrient availability for optimal cell growth
Release intact nucleic acids or live cells under biocompatible conditions
The compartment that changes what’s possible in single-cell biology.
Standard droplet microfluidics has enabled remarkable single-cell work — but the closed nature of conventional droplets has always imposed a ceiling on what workflows can achieve. Cells and reagents go in together, and nothing changes after that. Every complex multi-step protocol is a workaround for this constraint.
Semi-Permeable Capsules remove the ceiling. Cells and nucleic acids are retained. Small molecules — enzymes, buffers, primers — pass freely in and out. That exchange is what enables sequential chemistry, mid-workflow additions, and multi-omic profiling on the same cell, under optimal conditions at every step.
ANZ Market Manager - Research Genomics
Single-cell sequencing has transformed biology — but the most important questions in cancer, immunology, microbiology, and developmental biology don’t stop at gene expression. They require knowing how genomic variation drives transcriptional state, how DNA methylation connects to chromatin accessibility, how microbial genotype relates to function. Most single-cell platforms answer one of those questions at a time, on different cells, and ask you to infer the connections.
Atrandi’s SPC technology is most relevant where that inference is the limiting factor:
Direct DNA + RNA co-sequencing from single tumour cells connects somatic mutations to their transcriptional consequences without averaging across the population, enabling precise characterisation of clonal evolution and drug resistance mechanisms.
Profiling immune cell genotype and transcriptional state from the same cell, at scale, supports higher-resolution understanding of immune repertoire development and functional heterogeneity.
Single-cell microbial genomics at throughput that makes statistical inference meaningful — characterising strain-level diversity, horizontal gene transfer, and rare variants that metagenomics averages away.
Onyx’s open platform gives methods developers the tools to build and validate novel multi-omic workflows with full control over every microfluidic parameter, without being constrained by a closed application ecosystem.
Generate droplets and Semi-Permeable Capsules with full control over workflow design, encapsulation conditions, and throughput. Built for researchers developing next-generation screening workflows and single-cell applications.
Perform high-throughput droplet screening with multiparametric fluorescence selection and integrated sort verification. Designed for functional assays, protein engineering, and next-generation screening workflows.
Simplify SPC generation through an intuitive push-button platform designed for advanced single-cell analysis. Built for application-specific assays and reproducible results
Whole transcriptome single-cell RNA sequencing for fresh and frozen samples; combinatorial barcoding at cohort scale
Whole transcriptome snRNA-seq from archived FFPE tissue; instrument-free and multiplexing up to 96 samples per run
Spatial transcriptomics at nanoscale resolution; pairs with single-cell multi-omics for spatial gene expression mapping
Standard droplets are closed compartments — once formed, nothing goes in or out. SPCs are semi-permeable: they retain cells and high-molecular-weight nucleic acids while allowing small molecules such as enzymes, buffers, and primers to pass freely. This enables multi-step workflows and reagent exchange on the same encapsulated cell, which is not possible with conventional droplet microfluidics.
Flux is a push-button instrument designed for running Atrandi’s validated application kits with minimal setup — it encapsulates up to 100,000 cells across 8 samples in 30 minutes and is designed for researchers deploying established workflows. Onyx is an open research platform for groups developing custom droplet and SPC workflows, with full control over microfluidic parameters, reagent addition, and active droplet manipulation.
Current validated applications include Single-Cell Microbial Genomics (via the Single-Cell Microbial WGS Kit) and Single-Cell DNA + RNA Co-sequencing. Additional applications are in development. Contact Decode Science for the latest application roadmap.
Yes. Onyx is specifically designed for assay development, with independent syringe pump control, forcefield-based droplet manipulation, and an embedded UI that requires no software installation. The SPC Innovator Kit provides the reagents to begin SPC-based assay development.
Atrandi application kits produce libraries compatible with Illumina sequencing platforms. Contact Decode Science for guidance on sequencing depth and read length requirements for your specific application.
Yes. Onyx supports both standard droplet microfluidics and SPC generation, making it compatible with a broad range of liquid handling and encapsulation workflows.
Technical documentation and application notes are available from Decode Science on request, or via the Atrandi Biosciences resources portal.
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