Not every sequencing question belongs in a core facility. Whether you’re working at a remote field site, a resource-limited clinic, a teaching lab, or simply need a dedicated benchtop instrument without competing for shared equipment time, the DNBSEQ-E25 puts high-quality sequencing where you need it. Weighing just 15 kg and requiring nothing more than a power source, the E25 integrates microfluidic flow cell technology, CMOS-based detection, and self-luminous dye chemistry into a single compact unit that takes you from loaded sample to FASTQ output — no ancillary hardware, no bioinformatics workstation, no internet connection.
What sets the E25 apart from other portable or benchtop sequencers is the degree to which the entire workflow is self-contained. The built-in computing module handles base calling and report generation on-board, and the enhanced E25A model extends this to full data analysis pipelines for applications including whole-genome sequencing. There’s no washing required between runs, setup takes roughly 10 minutes, and the system supports both MGI and App-C libraries without primer substitutio
Plug it in,
load your sample,
and sequence.
No optical alignment, no external fluidics, no vibration-sensitive components to worry about.
CMOS detection module built directly beneath each spot on the microfluidic sequencing chip.
Eliminates the environmental sensitivity and maintenance overhead.
Handles sequencing and FASTQ output independently.
No server rack, no cloud upload, no network dependency — which also means no data breach risk for sensitive or clinical samples.
All our sequencing platforms can work as standalone systems from sample to result without any network connection, eliminating the risk of data breaches and ensuring complete data security.
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| Supports MGI and App-C libraries and requires no primer substitutions | ||||
|---|---|---|---|---|
| Reads* | Read Length** | Data Output | Run Time*** | Q30**** |
| 25 M | SE100 | 2.5 Gb | 5 hrs | >90% |
| 25 M | PE150 | 7.5 Gb | 20 hrs | >80% |
| * The maximum number of valid reads is obtained by running a specific standard library. Actual performance may vary depending on sample type and library construction method. ** SE100 consumables can be used for SE50 sequencing; PE150 consumables can be used for PE100 sequencing. Read lengths can be customized in the sequencer user interface. *** Run time includes DNB loading and FASTQ generation. Barcode sequencing time is excluded. **** Q30 and run time are averaged across the entire run using a standard library. Actual performance may vary depending on sample type, library quality, insert size, etc. | ||||
E25, E25A*
* For indoor use only.
Pathogen identification and outbreak tracking shouldn't wait for samples to ship back to a central facility. The E25 enables on-site whole-genome sequencing of bacterial and viral isolates — giving public health teams actionable genomic data in hours rather than days, directly at the point of need.
Ecological genomics, conservation biology, environmental monitoring, agricultural pathogen surveillance — all of these benefit from sequencing data generated close to the source. The E25's compact footprint, tolerance to variable environmental conditions, and independence from network infrastructure make it a practical tool for work outside traditional lab settings.
For groups developing or validating sequencing-based assays, the E25 offers a self-contained platform that simplifies compliance considerations. On-board data processing with no network dependency means sensitive patient-derived data never leaves the instrument, reducing IT and security overhead during assay development and pilot studies.
Giving students hands-on sequencing experience usually requires access to expensive, shared equipment with strict booking schedules. The E25 is affordable enough, compact enough, and simple enough to operate in a teaching lab context — making it possible to integrate real sequencing runs into graduate coursework and workshops.
Speak with a Decode Science specialist to determine whether the E25 fits your application, throughput needs, and operating environment. We'll help you understand the available configurations (E25 vs. E25A) and consumable options.
Use your existing MGI-compatible or App-C library preparation workflow. No primer substitutions or protocol modifications are required. The E25 supports SE100 and PE150 read configurations, with customisable read lengths set directly in the instrument interface.
Set up takes approximately 10 minutes. Load your DNB library onto the microfluidic flow cell, insert it into the instrument, and start the run. SE100 runs complete in around 5 hours; PE150 runs in approximately 20 hours.
FASTQ files are generated on-board at the end of each run. With the E25A model, downstream analysis — including alignment and variant calling for WGS applications — is handled directly on the instrument. Transfer data via USB or external drive.
ANZ Market Manager - Research Genomics
Dr Lyman Ngiam (PhD)
Postdoctoral Research Fellow at Centre for Environmental and Agricultural Solutions to Antimicrobial Resistance
"Both E25 and D4 have demonstrated the capability to generate sequencing results within a single day. The prebuilt library preparation protocol and streamlined sequencing workflow make the system highly user-friendly, enabling anyone with piptetting skill to perform sequencing runs with ease.
The simplified operation has significantly reduced preparation time. Notably, the system’s ability to work with low starting DNA concentrations makes it particularly versatile, allowing sequencing of a wide range of sample matrices without the need to obtain high DNA yields beforehand.
Overall, it is an efficient and practical platform, essentially a "load-and-go" system."
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