RNA-Seq Built for the Samples That Are Hardest to Sequence: Whole Transcriptome or Target Enrichment, Both Ready for FFPE
Twist’s RNA sequencing solutions are designed around demanding samples: low input, degraded RNA and FFPE tissue. The aim is high-quality, high-yield libraries from a short workflow with few steps. Two approaches are available. Whole Transcriptome gives unbiased profiling of all expressed RNA. Target Enrichment focuses your reads on all genes or a chosen subset, including damaged FFPE-derived fragments and full-length transcripts of expressed genes. Both start from the same RNA library prep chemistry and both include an FFPE repair module.
Input ranges from 1 to 1,000 ng, and library prep takes around 4.5 to 5 hours. Whole Transcriptome has an optional rRNA and globin depletion module, which extends the workflow to about 6 to 6.5 hours. For enrichment, you can choose an overnight hybridisation or a 5-hour Fast Hybridisation and Wash. The workflows are automation-friendly, so they scale to high-throughput sequencing. Custom RNA panels can also be designed for other organisms, including microbes. As your authorised Australian and New Zealand distributor for Twist Bioscience, Decode Science can help you choose the right route and support your protocol.
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50% on your chosen workflow
Capture all genes or a subset of genes of interest from your RNA libraries.
✓ Enrich damaged RNA fragments from FFPE
✓ Enrich full-length transcripts of expressed genes
✓ Create your own custom RNA panels
Profile everything expressed, without a predefined target list.
✓ FFPE compatible
✓ rRNA and globin gene depletion available
✓ Fast workflow
| If your goal is... | Choose | Why |
|---|---|---|
| Discovery across all expressed RNA | Whole Transcriptome | Unbiased, no predefined targets |
| Blood or globin-rich samples | Whole Transcriptome with Depletion | Removes rRNA and globin before sequencing |
| Gene expression, isoforms and coding variants from FFPE | Target Enrichment with RNA Exome | Focuses reads on protein-coding transcripts |
| Known and novel gene fusions | Target Enrichment with RNA Fusion Panel | Dedicated oncogenic fusion content |
| A specific gene list, or a non-human organism | Target Enrichment with Custom Panel | Panel built to your target list |
| Maximum samples per run at lower read depth | Target Enrichment | Fewer reads needed per sample for the same gene detection |
DNA tells you what a cell could do. RNA shows what it is doing, which is why expression, fusion and variant data from RNA are so valuable in oncology and translational research. The challenge is that clinical and archival samples are usually degraded, low in quantity or fixed in formalin. Twist’s RNA workflows are built to work with these samples, not around them.
The Twist RNA solutions are most relevant for:
Expression, fusion detection, variant calling and tumour signatures can be explored from a single RNA library.
The FFPE repair module and enrichment of damaged fragments make archival tissue usable.
Depletion of rRNA and globin removes the transcripts that would otherwise dominate your reads.
Custom panels can be designed for species beyond human.
Clinical Genomics Manager - ANZ & Country Manager - NZ
The transcriptome provides closer access to the real biological status of the tumour cell, in contrast to the more ‘static’ nature of DNA. The parallel analysis of gene expression, fusion detection, variant calling, tumour signatures, and much more, possible with one library prep and starting from three to five FFPE tissue slices makes the RNA exome kit a promising assay for future-proof implementation into our clinical routine.
Disclaimer: Results are specific to the institution where they were obtained and may not reflect the results achievable at other institutions.
Twist Target Enrichment technology is a robust workflow allowing us to accurately analyze patient samples with more than 80 fusion variants in a high-throughput lab workflow.
Disclaimer: Results are specific to the institution where they were obtained and may not reflect the results achievable at other institutions.
High-fidelity amplification-based library prep for target enrichment and challenging low-input or FFPE samples
Design and order target enrichment panels tailored to your gene list or genomic region of interest
High-fidelity, low-bias amplification for NGS libraries
The Twist RNA Exome is a target enrichment that generates transcriptome sequencing data from RNA extracted from many sources, including FFPE. It increases signal and needs fewer reads to detect the same number of genes. Its exon-aware design keeps isoforms and junctions detectable.
It’s an optional step within library prep that helps decrosslink formalin-fixed nucleic acid, making those sequences more available for library generation.
It’s designed for blood-containing samples and others that benefit from removing contaminating rRNA and globin. Contact Decode Science to confirm suitability for your species and sample type.
Yes. Twist’s bioinformatics team can design panels across many organisms, including microbes, and for a variety of applications.
Both workflows accept 1 to 1,000 ng.
Library prep takes 4.5 to 5 hours, or 6 to 6.5 hours with the depletion module. Target Enrichment adds an overnight (16 hour) hybridisation with Standard Hybridisation v2, or about 5 hours with Fast Hybridisation and Wash.
Choose Whole Transcriptome for unbiased discovery across all expressed RNA. Choose Target Enrichment when you want focused coverage of coding genes, fusions or a custom list, particularly from FFPE or when running many samples.
Yes. The workflows are designed to scale for high-throughput sequencing and suit automation.
Yes. These products are for research use only.
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