MosaiX High-Complexity Library Prep Kit – Early Access Promotion

November 24, 2025/in SeqWell/by Harshita Sharma

MosaiX High-Complexity Library Prep Kit ,
– Early Access Promotion

Be among the first to experience the newest in high-complexity library prep technology.

SeqWell has just launched the MosaiX High-Complexity Library Prep Kit, unveiled at ASHG, and we’re offering a limited-time early-access promotion for researchers in AU & NZ.

5/5

Early-Access Offer Includes:

How to Qualify?

Modified LongPlex™ Protocol (LongPlex XL)

November 17, 2025/in SeqWell/by Harshita Sharma

Modified LongPlex™ Protocol (LongPlex XL)

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This technical note outlines an alternative workflow for generating 10–15 kb HiFi reads from high-quality genomic DNA using the LongPlex Long Fragment Multiplexing Kit. In this approach, LongPlex is used to fragment and barcode samples, and PacBio’s Short Read Eliminator (SRE) is applied to size-select fragments >10 kb before SMRTbell® library preparation.

LongPlex uses plate-based transposase tagmentation for multiplexed fragmentation and barcoding, removing the need for mechanical shearing and allowing barcoded samples to be pooled before SMRTbell prep. This simplifies the workflow, increases throughput, and lowers library prep costs.

The standard LongPlex protocol generates 6–9 kb HiFi reads from high- to medium-quality DNA—ideal for microbial and other small-genome projects. However, users working with higher-quality DNA may want longer HiFi reads to maximize gigabase yield on PacBio systems.

This modified workflow is only suitable for high-quality DNA (Femto Pulse GQN30kb ≥7). Using degraded DNA will result in substantial sample loss during SRE size selection.

As the official distributor in Australia and New Zealand, Decode Science makes accessing genomics solutions straightforward. Our role is to connect your lab with advanced technologies, ensuring you get the right solution for your sequencing projects—delivered locally with support when you need it.

purePlex™ DNA Library Prep Kit

PRODUCTS

purePlex™ DNA Library Prep Kit

A benefit to purePlex is that, because of its simplicity, users can quickly and seamlessly incorporate the kit with existing methods for high-throughput pipetting.

Speed, Performance, and Auto-Normalization with Unique Dual Indexes

2.5-hour workflow for 96 samples, 45 min. hands-on time
Auto-normalization of read counts and insert size over 10-fold input range
Unique dual indices included

Key Features

Fast, flexible workflow with no requirement for full plate processing
Auto-normalization reduces QC burden, improves data consistency
Early pooling for easier sample handling
Reduced GC bias compared to other transposase-based methods
Significant cost and plastics savings

purePlex™ DNA Library Prep Kit Workflow seqWell

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ExpressPlex™ Library Prep Kit

January 17, 2024/in Partners, Plasmid and Amplicon Sequencing, SeqWell/by Harshita Sharma

PRODUCTS

ExpressPlex™ Library Prep Kit

Designed for quick turnaround of plasmid, amplicon, or synthetic construct sequencing, ExpressPlex* is the fastest high-throughput library preparation kit available (based on total time to prepare 96 – 384 samples).

90-min workflow with 30-min or less hands-on
Fragmentation, barcoding, and amplification in 1 step
No primers/barcodes to buy
Virtually cross-talk free
Up to 6,144 samples prepped and sequenced in 24 hours
NEW: 384-well ultra-high throughput version available

*Patents Pending

Benefits of using ExpressPlex

ExpressPlex allows you to spend your time on data and results, not pipetting. There are solutions for low-, medium-, and high-throughput labs. For ultra-high throughput users, we now offer 384-well versions that will enable you to multiplex up to 6,144 samples in a single run.

Go from extracted samples to libraries on the sequencer in < 1/2 a day
Sequence more samples for less
Easily automate your protocol
Train any lab tech, minimize chance for error
Choice of 96 or 384-well versions to fit your workflow
Reduce labor while increasing efficiency
Decrease chance for errors via minimal handling steps
Everything included: no complex supply chain management of barcodes and primers

ExpressPlex 96-Well Workflow

ExpressPlex 384-Well Workflow

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Tagify

January 17, 2024/in Gene Editing QC, Partners, SeqWell/by Harshita Sharma

Are You Confident in Your Gene Editing Results?

If you’re not validating every edit with high-quality sequence analysis, you’re guessing — and guessing has no place in CRISPR, TALEN, or ZFN workflows.

Accurate gene editing QC is essential for verifying edits, detecting off-target events, and protecting downstream research or therapeutic development. Robust sequencing isn’t optional — it’s your safety net.

Scalable, High-Fidelity QC for Every Gene Editing Workflow

On-Target & Off-Target Gene Editing QC

Tagify® Adapter-Loaded Transposase for High-Confidence Tagmentation

Accurate characterization of on-target and off-target editing events is non-negotiable. Insertions, deletions, inversions, translocations — every outcome needs to be detected and verified. Yet standardized QC methods for gene editing are still early-stage, especially for off-target analysis. Most teams end up navigating inconsistent protocols, variable reagent quality, and limited scalability.

Reliable, QC-Verified Reagents for Tagmentation-Based Gene Editing Assays

Tagify reagents remove the uncertainty. Each lot is fully QC-checked and delivered as ready-to-use or custom-loaded hyperactive Tn5 or TnX, seqWell’s next-generation transposase engineered for dependable performance.

With Tagify, you get:

Consistent tagmentation performance
Scalable workflows for high-throughput QC
Reagents optimized for sensitive off-target detection

Broad Compatibility with Leading Gene Editing QC Methods

Tagify reagents integrate seamlessly with widely used transposase-based assays including:

GUIDE-seq²
UDiTaS
CHANGE-seq
RGEN
TTIS-seq
and additional emerging QC workflows

*Some transposase-based methods require appropriate licensing.

Useful Webinars

If you’re looking to integrate these QC solutions into your pipeline, Decode Science can provide hands-on guidance and local expertise.

Happy Customer Feedback

"We’ve been asking for this. What’s great about Tagify is that it allows you to look at a specific place in the gene, and adapter concentration is taken care of. This system is really important because it provides us this opportunity to assess the consequences of gene editing in a semi-unbiased way. It shortens our process, makes it much more controlled, and lessens the amount of reagents we need to use.”

– Athea Vichas, Ph.D., Senior Principal Scientist of Gene Editing Analytical Development, Bristol Myers Squibb

“ExpressPlex is literally faster than Sanger. This changes everything for us. Basically, taking a two-day process to one day dramatically shortens time to data.”

– Henry Chan, Ph.D., Synthetic Biology Lead, Octant Bio

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plexWell LP 384

January 17, 2024/in Low-pass Whole Genome Sequencing, Microbial Sequencing, Partners, SeqWell/by Harshita Sharma

PRODUCTS

plexWell LP 384

The plexWell™ LP 384 kit is engineered for low-pass whole genome library preparation and sequencing, utilizing the enhanced plexWell workflow. The kit includes major reagents essential for library preparation, featuring Magwise™ paramagnetic beads (DNA polymerase not included). This kit allows efficient library preparation for more than one 96-well plate of samples and normalizes input DNA over a wide range of 5-25 ng.

plexWell™ LP 384 (Low-Pass Whole Genome) Highlights:

NGS multiplexed library generation kit designed for Illumina® platforms.
Features assay-ready 96-well fully-skirted low-profile PCR plates in sets of 4 (4 x 96).
Provides 2,304 (96 x 24) unique barcode combinations.
Includes 6 sets of 4 pool barcodes (PB Set A, B, C, D, E, or F).

Recommended Application: Ideal for low-depth whole genome/GBS (Genotyping by Sequencing) coupled with imputation and analysis software for comprehensive genomic studies.

plexWell™ Library Prep Workflow:

plexWell™ Library Prep Chemistry:

Ready To Order?

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SeqWell Portfolio

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1300 581 991

plexWell

January 17, 2024/in Microbial Sequencing, Partners, Plasmid and Amplicon Sequencing, SeqWell/by Harshita Sharma

PRODUCTS

plexWell

plexWell 96

The plexWell™ 96 (PW096) kit streamlines NGS multiplexed library generation for Illumina® platforms, housed in an assay-ready 96-well fully-skirted low-profile PCR plate. The kit includes major reagents, featuring Magwise™ paramagnetic beads (DNA polymerase not included), facilitating efficient library preparation for one 96-well plate inputs. Notably, it offers normalization of input DNA over a broad range of 3-30 ng, with a cost-saving volume-based pricing structure that can reduce total lab costs by 30-50%.

Key Features:

Comprehensive kit with Magwise™ beads.
Efficient library prep for one 96-well plate.
Normalization of input DNA (3-30 ng).
Volume-based pricing for cost savings.

Recommended Applications: Ideal for large-scale full-length viral surveillance, Synthetic Construct Sequencing (Amplicons, Plasmids, BACs, etc.), microbiome screening, microbial whole-genome sequencing, scRNA-seq (single-cell RNA sequencing), and low-depth whole-genome/GBS (genotyping by sequencing).

plexWell 384

The plexWell™ 384 (PW384) kit is a comprehensive solution for NGS multiplexed library generation on Illumina® platforms. Each kit includes major reagents, featuring Magwise™ paramagnetic beads (DNA polymerase not included), essential for efficient library preparation for more than one 96-well plate of samples. This kit offers normalization of input DNA across a wide range of 3-30 ng, with a cost-saving volume-based pricing structure, reducing total lab costs by 30-50%.

Key Features:

Comprehensive kit with Magwise™ paramagnetic beads.
Efficient library prep for more than one 96-well plate.
Normalization of input DNA (3-30 ng).
Cost-saving volume-based pricing.

Recommended Applications:

Large-scale full-length viral surveillance.
Synthetic Construct Sequencing (Amplicons, Plasmids, BACs, etc.).
Microbiome screening.
Microbial whole-genome sequencing.
scRNA-seq (single-cell RNA sequencing).
Low-depth whole-genome/GBS (genotyping by sequencing).

plexWell™ Library Prep Workflow:

plexWell™ Library Prep Chemistry:

Ready To Order?

Our team can help you in placing the order. Click below to get a quote and fast ordering.

SeqWell Portfolio

Have a question?

Get a call from your local Decode Science representative to help you find the best fit genomics products for you.

Or give us a call at:

1300 581 991

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