Entries by Harshita Sharma

Viral Panels

PRODUCTS

NGS Viral Panels

Comprehensive Viral Research Panel

The Twist Comprehensive Viral Research Panel is a highly versatile tool designed for comprehensive novel virus detection. It consists of 1,052,421 unique probes derived from reference sequences in databases like RefSeq, FluDB, and VIPR, covering a wide range of viral families affecting humans and animals. This panel can detect novel and evolved viral strains, including those related to specific outbreaks.

The panel demonstrates success in detecting highly divergent viral sequences, such as the spike region of a newly discovered coronavirus and a segment from an H1N1 influenza outbreak. With a mismatch sensitivity defined through various tests, it can capture highly evolved viral sequences with over 99.8% coverage.

Furthermore, the Twist Comprehensive Viral Research Panel allows for the enrichment of novel viruses, showcasing its ability to capture sequences with up to 10% variation. It also supports multiplex detection of diverse viruses, making it suitable for metagenomic applications in various sample types. In a co-infection assay, the panel successfully captured four different virus types spiked into human RNA with high efficiency, demonstrating its multiplexing capabilities.

NGS-Fixed-Panel-Comp-Virus-Research-Tree-2

NGS-Fixed-Panel-Comp-Virus-Research-Enrichment-Novel-Viruses

Respiratory Virus Research Panel

The Twist Respiratory Panel is a powerful tool designed for the detection of common human respiratory pathogens through a single test. It comprises 41,047 probes compiled from the GenBank database, allowing researchers to distinguish COVID-19 symptoms from those of other respiratory illnesses, whether influenza- or non-influenza-related. This panel facilitates high-resolution Next-Generation Sequencing (NGS) by enriching viral sequences, enabling the high-sensitivity detection of viral material, even in challenging samples, with more than 5000x enrichment. Post-enrichment, it achieves coverage of over 99.9% of the genome at 1X or greater.

The panel demonstrates its effectiveness in detecting viral standards from different viral families. Synthetic Viral Controls, when spiked into human carrier RNA, show significant enrichment, with over 70% of reads coming from viral genomes, representing at least a 2500-fold enrichment over the spiked-in content.

Furthermore, the Twist Respiratory Panel supports multiplexing for higher throughput and lower cost. Comparisons using multiplex and single-plex hybridization reactions at different viral titers show that 8-plex capture provides comparable efficient enrichment as a single-plex capture. This is demonstrated using the Twist Synthetic Viral Controls and the Twist Respiratory Virus Research Panel, showcasing the versatility and cost-effectiveness of multiplexing in this respiratory virus detection system.

NGS-Respiratory-Viral-Controls-Multiplexing

Ready To Order?

Our team can help you in placing the order. Click below to get a quote and fast ordering.

NGS Portfolio

Have a question?

Get a call from your local Decode Science representative to help you find the best fit genomics products for you.


    Or give us a call at:

    1300 581 991

    Exome Panels

    PRODUCTS

    Exome Panels

    Exome 2.0

    Twist Exome 2.0 is designed to detect rare and inherited diseases, as well as germline cancers. This panel’s high uniformity and low off-target rate deliver best-in-class sequencing efficiency, enabling quality data to be collected with less sequencing. With superior coverage of major genetic databases (RefSeq, CCDS, GenCode, Clinvar, ACMG73 and more) and the addition of clinically relevant non-coding pathogenic and likely pathogenic variants, Twist Exome 2.0 provides the value of multiple clinical panels all wrapped into one, easily customizable package.

    Twist Alliance VCGS Exome - 40.1 MB

    Victorian Clinical Genetics Services (VCGS) and Twist have collaborated to address the challenges physicians face in diagnosing heritable diseases when routine testing falls short. The result is the Twist Alliance VCGS Exome – 40.1 MB, a diagnostic panel drawing on VCGS’s extensive clinical genomics experience. This panel covers the entire exome, providing dedicated boosting of clinically relevant genes (Mendeliome), and is designed with special attention to additional coverage of clinically relevant loci beyond traditional gene coding regions.

    The Twist Alliance VCGS Exome – 40.1 MB is a highly uniform panel, considered best-in-class, and aims to facilitate the identification of alleles linked to heritable diseases. Whether used for carrier screening or pre- and post-natal testing, this panel is intended to streamline the diagnostic process.

    It’s important to note that the content of the Twist Alliance VCGS Exome – 40.1 MB has not been fully validated through the complete Twist workflow, and for guidance on assay set-up, contacting Twist Customer Support is recommended. Additionally, this panel is not ISO-13485 certified.

    Twist Alliance VCGS Exome - 40.1 MB​

    Twist Alliance Clinical Research Exome - 34.9 MB

    The Twist Alliance Clinical Research Exome, developed in collaboration with the Broad Institute Genomics Platform, is a versatile tool designed for comprehensive exome sequencing.

    Utilizing validated data from clinical patient samples, this panel is constructed on Twist’s flexible Next-Generation Sequencing (NGS) platform, allowing easy and rapid customization of content. It provides a thorough examination of the exome, with additional enrichment focusing on clinically relevant areas associated with cancer, rare diseases, and inherited conditions.

    Leveraging the exceptional uniformity of Twist NGS probes, the assay achieves cost and throughput efficiency. The Broad Institute Genomics Platform has already processed over 250,000 samples, showcasing the panel’s effectiveness and keeping the institute at the forefront of exome sequencing.

    The panel’s design encompasses the Twist Core exome, the mitochondrial genome, and validated coding and non-exonic regions of interest, including ACMG73 genes, supplemental coverage of OMIM and COSMIC regions, and specific targets defined by Broad. It offers a comprehensive solution for a range of research applications.

    Important to note, the content of the Twist Alliance Clinical Research Exome – 34.9 MB has not been fully validated through the complete Twist workflow. For guidance on assay set-up, users are advised to contact Twist Customer Support. Additionally, this panel is not ISO-13485 certified.

    Mouse Exome Panel

    The mouse is an extremely important model system for studying genetic variation, tumor mutations, and phenotypic outcomes as well as the therapeutic effect of pharmaceutical agents. As genetic variant databases are continuously updated, the Twist Mouse Exome panel is thoughtfully designed and built from the most current databases. When combined with Twist’s expanding portfolio of library preparation and enrichment reagents, the complete toolset allows researchers to achieve industry-leading coverage across target regions while optimizing sequencing cost and sample throughput.

    Mouse Strain

    Twist Alliance Canine Exome - 40.5 MB

    Understanding the genetic variations among dog breeds is crucial for unraveling the genetic regulation of traits and understanding the basis and progression of diseases in dogs. Comprehensive gene panels, such as exomes, play a pivotal role in enhancing veterinary diagnostics and associated clinical medicine. These Next-Generation Sequencing (NGS) panels, like the Twist Alliance Canine Exome developed in collaboration with the Broad Institute’s Karlsson lab, are essential tools for advancing canine genomic research. They contribute to improved understanding of canine cancers and potential therapeutics.

    The Twist Alliance Canine Exome panel is designed to achieve the following objectives:

    1. Covers coding exons of canine genes.
    2. Facilitates comparative genomic studies between canine and human genomes.
    3. Includes regions of known importance in human cancers.
    4. Allows for cost-effective deep sequencing.

    Furthermore, canine genomic research has demonstrated benefits for human medical research, revealing genetic similarities between human and canine tumors, such as Copy Number Variations (CNVs), differential gene expressions, and structural chromosome abnormalities. This interdisciplinary approach underscores the potential for insights gained in canine genetics to contribute to advancements in human medicine.

    NGS Portfolio

    Have a question?

    Get a call from your local Decode Science representative to help you find the best fit genomics products for you.


      Or give us a call at:

      1300 581 991

      STOmics Offline Software

      PRODUCTS

      STOmics Offline Software

      STOmics Offline Software

      Spatial Omics Analysis Suite Overview: ImageStudio, SAW, and StereoMap

      ImageStudio:

      1. ImageStudio is a SpatioTemporal omics offline image processing software.
      2. It assesses image quality, focusing on sharpness and track lines, determining suitability for downstream data analysis.
      3. The manual adjustment module addresses scenarios beyond the software’s capabilities.

      SAW (Stereo-seq Analysis Workflow):

      1. SAW integrates Stereo-seq spatial group gene expression analysis tools.
      2. It reduces and visualizes spatial expression information from sequencing data on microarrays.
      3. Spatial expression matrices generated by SAW are suitable for downstream analysis.

      StereoMap:

      1. StereoMap is a high-definition visual desktop software designed for Stereo-seq data analysis results.
      2. It visualizes STOmics’ SpatioTemporal omics technologies, allowing further exploration through various tools.
      3. GEF matrix, image RPI and IPR data, and clustering results from SAW can be displayed in StereoMap.

      STOmics Offline Software

      Ready To Order?

      Our team can help you in placing the order. Click below to get a quote and fast ordering.

      STOmics Software Portfolio

      STOmics Offline Software

      STOmics Cloud

      Have a question?

      Get a call from your local Decode Science representative to help you find the best fit genomics products for you.


        Or give us a call at:

        1300 581 991

        STOmics Cloud

        PRODUCTS

        STOmics Cloud

        STOmics Cloud

        STOmics Cloud is a spatial-temporal data analysis platform for managing and analyzing multi-omics data. The platform is centered around projects, enabling users to seamlessly integrate data and tools into their projects, ensuring traceable analysis processes, reproducible results, knowledge sharing, and project collaboration. Additionally, STOmics Cloud offers a user-friendly portal interface, providing code-free data analysis, high-resolution visualization, and personalized analysis services.

        STOmics Cloud

        What You Can Achieve



        Data Analysis



        Data Visualization



        Data Storage



        Data Sharing

        STOmics Cloud Workflow

        STOmics Cloud Workflow

        Ready To Order?

        Our team can help you in placing the order. Click below to get a quote and fast ordering.

        STOmics Software Portfolio

        STOmics Offline Software

        STOmics Cloud

        Have a question?

        Get a call from your local Decode Science representative to help you find the best fit genomics products for you.


          Or give us a call at:

          1300 581 991

          Stereo-seq Transcriptomics for Large Chip Designs

          PRODUCTS

          Stereo-seq Transcriptomics for Large Chip Designs

          Stereo-seq Transcriptomics for Large Chip Designs

          STOmics Stereo-seq Transcriptomics for Large Chip Designs (LCD) pioneers the whole transcriptome study for entire tissue sections. Stereo-seq for Large Chip Designs is firstly offered to the market – 1cm x 2cm, 2cm x 2cm, 2cm x 3cm. Compatible for all species (FF samples), it enables a “tissue-to-data” solution through in situ capture of the whole transcriptome, at nanoscale resolution and centimeter-sized field of view.

          Stereo-seq Transcriptomics for Large Chip Designs

          Stereo-seq Transcriptomics for Large Chip Designs Workflow

          Stereo-seq Transcriptomics for Large Chip Designs workflow

          Ready To Order?

          Our team can help you in placing the order. Click below to get a quote and fast ordering.

          STOmics Kits Portfolio

          Stereo-seq Transcriptomics solution

          Stereo-seq Transcriptomics mIF solution

          Stereo-seq Transcriptomics H&E solution

          Stereo-seq Transcriptomics for Large Chip Designs

          Have a question?

          Get a call from your local Decode Science representative to help you find the best fit genomics products for you.


            Or give us a call at:

            1300 581 991

            Stereo-seq Transcriptomics H&E solution

            PRODUCTS

            Stereo-seq Transcriptomics H&E solution

            Stereo-seq Transcriptomics H&E solution

            Unlock the potential of integrating tissue phenotyping and spatial heterogeneity profiling of molecular information. By incorporating H&E-stained tissue morphological information, Stereo-seq Transcriptomics H&E solution will better assist with tissue type identification, profiling of specific tissue regions of interests (ROIs), and conducting downstream differential analysis between selected ROIs.

            Stereo-seq Transcriptomics H&E solution

            Stereo-seq Transcriptomics H&E solution workflow

            Stereo-seq Transcriptomics H&E solution workflow

            Ready To Order?

            Our team can help you in placing the order. Click below to get a quote and fast ordering.

            STOmics Kits Portfolio

            Stereo-seq Transcriptomics solution

            Stereo-seq Transcriptomics mIF solution

            Stereo-seq Transcriptomics H&E solution

            Stereo-seq Transcriptomics for Large Chip Designs

            Have a question?

            Get a call from your local Decode Science representative to help you find the best fit genomics products for you.


              Or give us a call at:

              1300 581 991

              Stereo-seq Transcriptomics mIF solution

              PRODUCTS

              Stereo-seq Transcriptomics mIF solution

              Stereo-seq Transcriptomics mIF solution

              STOmics Stereo-seq Transcriptomics Set for Chip-on-a-slide enables a “tissue-to-data” solution through in situ capture of the whole transcriptome, at nanoscale resolution and centimeter-sized field of view. Stereo-seq Transcriptomics Solution utilizes DNB patterned array chips loaded with spatially-barcoded probes that capture and prime poly-adenylated mRNA from tissue sections in situ. Each cDNA synthesized from mRNA captured on a particular spot is linked to its spatially-barcoded probe, allowing subsequent gene expression mapping of a tissue section following sequencing and visualization analysis using the StereoMap visualization platform.

              Stereo-seq Transcriptomics mIF solution

              Stereo-seq Transcriptomics mIF solution workflow

              Stereo-seq Transcriptomics mIF solution workflow

              Ready To Order?

              Our team can help you in placing the order. Click below to get a quote and fast ordering.

              STOmics Kits Portfolio

              Stereo-seq Transcriptomics solution

              Stereo-seq Transcriptomics mIF solution

              Stereo-seq Transcriptomics H&E solution

              Stereo-seq Transcriptomics for Large Chip Designs

              Have a question?

              Get a call from your local Decode Science representative to help you find the best fit genomics products for you.


                Or give us a call at:

                1300 581 991

                Stereo-seq Transcriptomics solution

                PRODUCTS

                Stereo-seq Transcriptomics solution

                Stereo-seq Transcriptomics solution

                STOmics Stereo-seq Transcriptomics Set for Chip-on-a-slide enables a “tissue-to-data” solution through in situ capture of the whole transcriptome, at nanoscale resolution and centimeter-sized field of view. Stereo-seq Transcriptomics Solution utilizes DNB patterned array chips loaded with spatially-barcoded probes that capture and prime poly-adenylated mRNA from tissue sections in situ. Each cDNA synthesized from mRNA captured on a particular spot is linked to its spatially-barcoded probe, allowing subsequent gene expression mapping of a tissue section following sequencing and visualization analysis using the StereoMap visualization platform.

                Stereo-seq Transcriptomics solution

                Stereo-seq Transcriptomics solution Workflow

                Stereo-seq Transcriptomics solution Workflow

                Ready To Order?

                Our team can help you in placing the order. Click below to get a quote and fast ordering.

                STOmics Kits Portfolio

                Stereo-seq Transcriptomics solution

                Stereo-seq Transcriptomics mIF solution

                Stereo-seq Transcriptomics H&E solution

                Stereo-seq Transcriptomics for Large Chip Designs

                Have a question?

                Get a call from your local Decode Science representative to help you find the best fit genomics products for you.


                  Or give us a call at:

                  1300 581 991