DNBSEQ-G99
/in MGI, Partners, Sequencing Platforms/by Harshita Sharma
Speed matters in genomics — and not just for urgent clinical samples. Faster turnaround means tighter experimental iteration, shorter time-to-answer for surveillance workflows, and more efficient use of lab time across every application. The DNBSEQ-G99 is engineered around this principle. Delivering PE150 sequencing with Q40 base quality in approximately 12 hours, it is one of the fastest benchtop sequencers in its throughput class. That speed comes from coordinated advances in biochemistry, optics, fluidics, and temperature control — not from cutting corners on data quality. Q40 accuracy (fewer than 1 in 10,000 miscalled bases) is maintained across key read lengths using MGI’s StandardMPS 2.0 reagents, giving you high-confidence data on the first pass.
What gives the G99 its breadth is the three-tier flow cell system. The FCS (40M reads) handles small targeted panels and rapid-turnaround jobs. The FCL (80M reads) covers the mid-range — oncology panels, microbial genomes, RNA-seq, methylation, and more — with read lengths from SE50 through PE300 and even SE400 for forensics and long-amplicon work. And the FCU (200M reads) extends into whole-exome sequencing territory, delivering up to 120 Gb per flow cell at PE150 and supporting batches of 8 or more exomes per run. With dual flow cell slots running independently, you can pair any two of these formats in a single run — matching throughput to demand without wasting capacity or waiting for a full batch.
PE150 at Q40 in 12 Hours
For labs running oncology panels, infectious disease assays, or any workflow where next-day results matter, this means samples loaded in the morning can have FASTQ files ready by the end of the day.
Three Flow Cell Formats, One Instrument
FCS (40M reads), FCL (80M reads), and FCU (200M reads) — covering applications from small targeted panels through to whole-exome sequencing.
Read Lengths from SE50 to SE400 — Including PE300
SE50 for NIPT and low-pass WGS. SE100 and PE150 for the bulk of standard applications. PE300 for 16S metagenomics and long-amplicon work, delivering up to 240 Gb per run on dual FCU flow cells. And SE400 for forensic DNA applications and extended-read requirements.

Chris Wicky
Clinical Genomics Manager - ANZ & Country Manager - NZ
Need help matching flow cell format and read length to your application? Our sequencing specialists can model your sample batching, per-sample costs, and turnaround times — reach out and we’ll respond at the earliest.
Your Data Security is Our Top Priority
All our sequencing platforms can work as standalone systems from sample to result without any network connection, eliminating the risk of data breaches and ensuring complete data security.
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Ebru Boslem
ANZ Market Manager - Research Genomics
For Clinical & Diagnostic Genomics Labs
Turnaround time is a clinical deliverable, not a convenience metric. PE150 in 12 hours with Q40 accuracy means panels and exomes can be reported within clinically relevant timeframes. The G99's App-D compatibility with Illumina libraries simplifies validation and transition for labs moving from existing platforms, and the optional bioinformatics module (G99A) enables on-board analysis without external compute infrastructure — reducing IT overhead and data security complexity.
For Oncology Panel & Precision Medicine Groups
Somatic variant calling at low allele frequencies demands both accuracy and depth. Q40 data quality reduces false positive calls that waste follow-up resources, while the G99's flexible batching — from a handful of samples on the FCS to larger cohorts on the FCU — means you're not over-sequencing small batches or under-powering large ones. Methylation-based cancer detection panels are equally well served, with PE150 support at the depths these assays require.
For Infectious Disease & Public Health Surveillance
Pathogen identification, resistance profiling, metagenomic screening, and 16S community analysis all run on the G99. SE50 and SE100 modes handle rapid pathogen detection with same-day turnaround. PE300 on the FCL or FCU supports full 16S sequencing for microbiome and environmental monitoring. ATOPlex respiratory and COVID-19 panel workflows are directly supported, and the dual flow cell system lets you run surveillance samples alongside research projects without scheduling conflicts.
For Research Groups Running Diverse Applications
If your lab runs RNA-seq one week, targeted panels the next, and occasionally needs an exome or a small genome, the G99 eliminates the need to juggle multiple instruments or batch dissimilar projects together. Three flow cell options, read lengths from SE50 to SE400, and dual independent flow cell slots mean virtually any standard NGS application fits on this single benchtop platform.
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